Collagen IV Antibody - BSA Free

Novus Biologicals | Catalog # NB120-6586

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Feline

Cited:

Human, Mouse, Rat, Feline

Applications

Validated:

Knockout Validated, Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Super-Resolution Microscopy

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, IF/IHC, Super-Resolution Microscopy

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Collagen IV Primary Antibodies »

Product Specifications

Immunogen

Collagen IV from human and bovine placenta (Uniprot: P02462)

Reactivity Notes

This antibody reacts with most mammalian Collagen IV and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Rat reactivity reported in scientific literature (PMID: 16774905)

Feline reactivity reported in scientific literature (PMID: 33091431).

Localization

Secreted and Cell surface (Potential).

Specificity

Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen IV and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Collagen IV Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Collagen IV Antibody [NB120-6586]

Immunocytochemistry/ Immunofluorescence: Collagen IV Antibody [NB120-6586]

Collagen-IV-Antibody-Immunocytochemistry-Immunofluorescence-NB120-6586-img0017.jpg
Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - Analysis of Collagen IV in various paraffin embedded human tissues. Image courtesy of product review submitted by Daniel Pirici.
Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - Collagen IV antibody at 1:400, 45 min RT showed strong staining in FFPE sections of human kidney (left) with strong red staining observed in glomeruli and liver (right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 100C for 20 minutes for antigen retrieval.
Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - Analysis of Collagen IV in paraffin embedded mouse cervix tissue using anti-Collagen IV antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - Analysis using the Biotin conjugate of NB120-6586. Staining of Collagen IV (red) and Insulin (green) in mouse pancreas. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Frozen: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Frozen: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Frozen: Collagen IV Antibody [NB120-6586] - Mouse liver tissue. Collagen IV antibody at 1:200 dilution. Overnight incubation, no permeabilization. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Frozen: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Frozen: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Frozen: Collagen IV Antibody [NB120-6586] - Mouse brain tissue. Antibody at 1:500. IHC-Fr image submitted by a verified customer review.
Knockout Validated: Collagen IV Antibody [NB120-6586]

Immunocytochemistry/ Immunofluorescence: Collagen IV Antibody [NB120-6586]

Immunocytochemistry/ Immunofluorescence: Collagen IV Antibody [NB120-6586] - Collagen IV was detected in immersion fixed THP-1 (positive) but not THP-1 KO (negative) cells using 10 ug/mL Rabbit Anti-Human/Bovine Polyclonal Antibody (Catalog # NB120-6586) for 3 hours at room temperature. Cells were stained with the NorthernLights(TM) 557-conjugated Donkey anti-Rabbit IgG Secondary Antibody [NL557] (red; Catalog # NL004) and counterstained with DAPI (blue).

Applications for Collagen IV Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:50000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1:100

Simple Western

1:50

Western Blot

1:1000-1:10000
Application Notes
This product has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.
Use in Immunohistochemistry Free-Floating reported in scientific literature (PMID: 32245949).
See Simple Western Antibody Database for Simple Western validation: Tested in Lungs, separated by Size, antibody dilution of 1:50

Reviewed Applications

Read 7 reviews rated 4.9 using NB120-6586 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.

Background: Collagen IV

Collagens comprise a large family of insoluble extracellular glycoproteins that are essential components of connective tissues such as tendons, ligaments, cartilage, bone and skin. The mature polypeptides are secreted as coiled, left-handed helices that subsequently assemble into rope-like collagen fibers. Collagen I is a fibril-forming collagen that requires N- and C- terminal processing. Collagen IV is a network forming collagen whose C-terminus forms dimers and N-terminus forms tetramers.

Alternate Names

BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR

Gene Symbol

COL4A1

UniProt

P02462 (Human)

Additional Collagen IV Products

Product Documents for Collagen IV Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Collagen IV Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Collagen IV Antibody - BSA Free

Customer Reviews for Collagen IV Antibody - BSA Free (7)

4.9 out of 5
7 Customer Ratings
5 Stars
86%
4 Stars
14%
3 Stars
0%
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1 Stars
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Customer Images

Showing  1 - 5 of 7 reviews Showing All
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  • Collagen IV alpha 1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: blade
    Species: Human
    Verified Customer | Posted 10/09/2019
    Collagen IV Antibody - BSA Free NB120-6586
  • Collagen IV alpha 1 Antibody
    Name: Abhijit Nirwane
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 09/10/2019
    1:500
    Collagen IV Antibody - BSA Free NB120-6586
  • Collagen IV alpha 1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Liver
    Species: Mouse
    Verified Customer | Posted 08/09/2019
    Dilution 1:200 Incubation: Overnight 4C No permeabilization
    Collagen IV Antibody - BSA Free NB120-6586
  • Collagen IV alpha 1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Connective tissue
    Species: Feline
    Verified Customer | Posted 02/18/2018
    All images were captured using Leica SP8 conforcal microscope with X20 objective. Those primary antibodies were used at 1:200 dilution.
    Collagen IV Antibody - BSA Free NB120-6586
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse cervix
    Species: Mouse
    Verified Customer | Posted 04/27/2016
    Mouse cervix
    Collagen IV Antibody - BSA Free NB120-6586
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Verified Customer | Posted 09/15/2012
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human tissue
    Species: Human
    Verified Customer | Posted 09/15/2012
    Collagen IV Antibody - BSA Free NB120-6586

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