Collagen IV Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-26549

Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human, Mouse, Rat, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

Azide and BSA Free
View all Collagen IV Primary Antibodies »

Product Specifications

Immunogen

Goats were hyperimmunized with human type IV collagen.

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 27417518), Mouse reactivity reported in scientific literature (PMID: 32479919), Rat reactivity reported in scientific literature (PMID: 30362896).

Specificity

Reacts with conformational determinants on human type IV collagen as demonstrated by ELISA. May react with type IV collagen from other species. Exhibits <10% cross reactivity with collagen type I, II, III, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Collagen IV Antibody - Azide and BSA Free

Immunohistochemistry-Frozen: Collagen IV Antibody [NBP1-26549]

Immunohistochemistry-Frozen: Collagen IV Antibody [NBP1-26549]

Immunohistochemistry-Frozen: Collagen IV Antibody [NBP1-26549] - Left panel: NBP1-26549 signal, identifying Collagen IV in extracellular basement membrane of brain endothelial cells. Right panel: counterstained image with DAPI. Mouse brain tissue (12um thickness) was incubated overnight with NBP1-26549 (1:400) in PBS 1x at 4C. The day after, the slide was washed 3 x PBS 1x and then incubated with secondary anti-goat antibody (Alex Fluor 568-conjugated), 1:1000 for 2 hours at r.t. After counterstaining with DAPI, image was acquired with confocal microscopy. Left panel: NBP1-26549 signal, identifying Collagen IV in extracellular basement membrane of brain endothelial cells. Right panel: counterstained image with DAPI. Image from verified customer review.
Immunohistochemistry-Paraffin: Collagen IV Antibody [NBP1-26549]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NBP1-26549]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NBP1-26549] - Human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (left) and Goat Anti-Type IV Collagen-UNLB; (right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP DAB, and hematoxylin.
Immunohistochemistry: Collagen IV Antibody [NBP1-26549]

Immunohistochemistry: Collagen IV Antibody [NBP1-26549]

Collagen-IV-Antibody-Immunohistochemistry-NBP1-26549-img0002.jpg
Immunohistochemistry: Collagen IV Antibody [NBP1-26549]

Immunohistochemistry: Collagen IV Antibody [NBP1-26549]

Collagen-IV-Antibody-Immunohistochemistry-NBP1-26549-img0003.jpg
Collagen IV Antibody

Immunohistochemistry: Collagen IV Antibody [NBP1-26549] -

Immunohistochemistry: Collagen IV Antibody [NBP1-26549] - Photographs showing H&E staining & immunostaining for Col-I, Col-III, Col-IV & Col-V in the rhesus macaque & baboon intramural tube. Images of open fallopian tube (a, c, e, g, i, k, m, o, q & s) were obtained from control animals. Occluded tube (b, d, f, h, j, l, n, p, r & t) were from animals treated with 5% PF. Arrows indicate fibrotic response. All images were captured at original magnification ×200. Insets (frame r & s) show negative control with an irrelevant antibody. Treatment with PF increased collagen immunoreactivity in the lamina propria of the intramural tube. Image collected & cropped by CiteAb from the following publication (https://linkinghub.elsevier.com/retrieve/pii/S0010782416301445), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Collagen IV Antibody

Immunohistochemistry: Collagen IV Antibody [NBP1-26549] -

Immunohistochemistry: Collagen IV Antibody [NBP1-26549] - Photographs showing H&E staining & immunostaining for Col-I, Col-III, Col-IV & Col-V in the rhesus macaque & baboon intramural tube. Images of open fallopian tube (a, c, e, g, i, k, m, o, q & s) were obtained from control animals. Occluded tube (b, d, f, h, j, l, n, p, r & t) were from animals treated with 5% PF. Arrows indicate fibrotic response. All images were captured at original magnification ×200. Insets (frame r & s) show negative control with an irrelevant antibody. Treatment with PF increased collagen immunoreactivity in the lamina propria of the intramural tube. Image collected & cropped by CiteAb from the following publication (https://linkinghub.elsevier.com/retrieve/pii/S0010782416301445), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Collagen IV Antibody - Azide and BSA Free

Western Blot: Collagen IV Antibody - Azide and BSA Free [NBP1-26549] -

MiR-29a regulation of target gene expression in cochlea from miR-29a+/+ and miR-29a–/– mice. (A–G) With beta -actin as a control, COL4A1, COL4A2, COL4A3, COL4A4, COL4A5, LAMB2, and LAMC1 protein levels in cochlea were analyzed by Western blot. The independent t-test was used to compare data between two groups [COL4A1, t(4) = 9.920, P < 0.001; COL4A2, t(4) = 3.847, P = 0.018; COL4A3, t(4) = 3.389, P = 0.028; COL4A4, t(4) = 2.842, P = 0.047; COL4A5, t(4) = 3.782, P = 0.019; LAMB2, t(4) = 3.915, P = 0.017; LAMC1, t(4) = 3.088, P = 0.037]. (H) The mRNA expression levels of target genes as measured by qRT-PCR [independent t-test, Col4a1, t(4) = 6.050, P = 0.004; Col4a2, t(4) = 13.145, P < 0.001; Col4a3, t(4) = 3.735, P = 0.02; Col4a4, t(4) = 7.238, P = 0.002; Col4a5, t(4) = 13.141, P < 0.001; Lamb2, t(4) = 8.529, P = 0.001; Lamc1, t(4) = 3.126, P = 0.035]. All data are shown as the mean +/- SD, n = 3 biological replicates, *P < 0.05, **P < 0.01, and ***P < 0.001 between two groups. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37275774), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Collagen IV Antibody - Azide and BSA Free

Western Blot: Collagen IV Antibody - Azide and BSA Free [NBP1-26549] -

Nimbidiol treatment normalized the expression of collagen IV, fibronectin and elastin in the diabetic kidney. Representative images from (A) Semi-quantitative RT-PCR analyses showing gene expression and (B) western blot analyses showing protein expression of Col IV, fibronectin and elastin in the kidney. The bar diagrams represent the fold change from WT + Saline. Data are mean +/- SD (n = 6/group). *p < 0.05 versus WT + Saline, WT + Nimbidiol and Akita + Nimbidiol, †p < 0.05 versus Akita + Saline. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36522378), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Collagen IV Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Collagen IV Antibody - Azide and BSA Free [NBP1-26549] -

Decreased angiogenesis and increased integrin/basement membrane signaling in TNF lungs.(A) Loss of angiogenesis signaling in TNF-Tg mice. MultiNicheNet was used to identify ligand-receptor pairs that were specific to either WT or TNF conditions. WT lungs demonstrated strong Tek, Flt1, Kdr, and Ednrb interactions with Angt1, Vegfa, Gpc3, and Edn3, which were absent TNF-Tg conditions. (B) Increased TGF-beta /integrin/basement membrane signaling in TNF-Tg lungs. TNF-Tg lungs demonstrated the presence of interactions between Itga2 (primarily in gCAP2 cells) and multiple collagen genes, Hspg2, and Tgfb1. (C) Decreased expression of Flt1 and Tek and increased expression of Col4a2 and Itga2 in TNF-Tg gCAP cells. Violin plots demonstrate expression across cell types. (D) Immunostaining confirms increased expression of Col4a1, Itga2, and Igfbp7 in TNF-Tg lungs. Original magnification × 200. (E) Circos plots indicating most differentially regulated ligand-receptor pairs ascertained by MultiNicheNet in WT lungs (left) and TNF-Tg (right) lungs; sender cells are listed on the bottom, while arrows point to receiver cells on the top. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40569693), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Collagen IV Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Collagen IV Antibody - Azide and BSA Free [NBP1-26549] -

Nimbidiol inhibited upregulation of collagen IV and fibronectin, and downregulation of elastin expression in the diabetic kidney. Representative immunofluorescence images of the renal cortex showing expression of (A) Col IV, (B) fibronectin and (C) elastin. The nuclear counterstaining was performed using DAPI (blue). The bar diagrams represent the fold change in the fluorescence intensity from WT + Saline for (D) Col IV (E) fibronectin and (F) elastin. Data are mean +/- SD (n = 6/group). *p < 0.05 versus WT + Saline, WT + Nimbidiol and Akita + Nimbidiol, †p < 0.05 versus Akita + Saline. Scale bar: 20 um; magnification × 60. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36522378), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Collagen IV Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:1000-1:4000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
IHC, IHC-FR reported in (PMID: 32029859), ICC/IF reported in (PMID: 32479919), IHC-P reported in (PMID: 27417518).

Reviewed Applications

Read 1 review rated 5 using NBP1-26549 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1M BBS (pH 8.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.4 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Collagen IV

Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas. This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Alternate Names

BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR

Gene Symbol

COL4A1

Additional Collagen IV Products

Product Documents for Collagen IV Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Collagen IV Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Collagen IV Antibody - Azide and BSA Free

Customer Reviews for Collagen IV Antibody - Azide and BSA Free (1)

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  • Collagen IV Antibody
    Name: STEFANO Benvegnu
    Application: Immunohistochemistry-Frozen
    Sample Tested: Adult mouse brain tissue
    Species: Mouse
    Verified Customer | Posted 05/24/2022
    Left panel: NBP1-26549 signal, identifying Collagen IV in extracellular basement membrane of brain endothelial cells. Right panel: counterstained image with DAPI.
    Mouse brain tissue (12um thickness) was incubated overnight with NBP1-26549 (1:400) in PBS 1x at 4C. The day after, the slide was washed 3 x PBS 1x and then incubated with secondary anti-goat antibody (Alex Fluor 568-conjugated), 1:1000 for 2 hours at r.t. After counterstaining with DAPI, image was acquired with confocal microscopy. Left panel: NBP1-26549 signal, identifying Collagen IV in extracellular basement membrane of brain endothelial cells. Right panel: counterstained image with DAPI.
    Collagen IV Antibody - Azide and BSA Free NBP1-26549

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