Complement Component C3a is an anaphylotoxin polypeptide that is proteolytically generated from C3. C3a formation is common to all three pathways of complement activation: classical (antibody-mediated), lectin and alternative. It binds C3a R on myeloid peripheral blood leukocytes, activated lymphocytes, endothelial and internal organ epithelial cells. It activates mast cells, neutrophils, and macrophages, enhances Th2 inflammatory reactions, promotes Th1 cell maturation, and inhibits Treg differentiation. It also stimulates leukocyte chemotaxis and smooth muscle contraction. Removal of the C-terminal arginine generates C3adesArg/ASP that binds the C5L2 receptor (GPR77) and stimulates triglyceride synthesis in adipocytes.
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Recombinant Rabbit Complement Component C3a Protein
Novus Biologicals | Catalog # NBP3-57452
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Key Product Details
Applications
Western Blot, ELISA, Cell Culture
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Product Specifications
Description
A recombinant protein corresponding to 77 aa of Rabbit Complement Component C3a.
Source: Yeast
Amino Acid Sequence: SRRRRSLRRL EAKRDAVNKF KTELERKCCE AGLKENPAGL ACEERTRHVL HGPSCMAAFL DCCQLSETLT REAREER (77)
Stable for up to 12 months from date of receipt at -20C. Stable for 3 months when stored in working aliquots with a carrier protein at -20C. Avoid repeated freeze/thaw cycles.
Purity
>98%, by SDS-PAGE
Endotoxin Level
No affinity tags & naturally endotoxin-free
Predicted Molecular Mass
8.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications
Enzyme-linked Immunosorbent Spot (ELISPOT)
ELISA Standard (Matched Pair) (ELISA(Sta))
ELISA Standard (Matched Pair) (ELISA(Sta))
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation, and Storage
NBP3-57452
| Formulation | Lyophilized in 1-5X PBS containing 10% Trehalose |
| Preservative | 10% Trehalose |
| Concentration | Lyoph |
| Reconstitution | Reconstitute in sterile PBS with at least 0.1% carrier protein |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20C. Avoid freeze-thaw cycles. |
Calculators
Background: Complement Component C3a
Alternate Names
Anaphylatoxin, C3
Gene Symbol
C3
Additional Complement Component C3a Products
Product Documents for Recombinant Rabbit Complement Component C3a Protein
Certificate of Analysis
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Product Specific Notices for Recombinant Rabbit Complement Component C3a Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Related Research Areas
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