Corticosterone, also known as Kendall's compound B and Reichstein substance B, is a glucocorticoid secreted by the adrenal gland cortex. It is produced in response to adrenal cortex stimulation by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Stress increases corticosteroid production. Corticosterone level is measured as an indicator of stress in patients with dietary restrictions, long-term memory impairment, and burn injuries.
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Corticosterone Parameter Assay Kit
R&D Systems | Catalog # KGE009
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Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (10 µL)
Sensitivity
0.047 ng/mL
Assay Range
0.103-25 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
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Product Summary for Corticosterone Parameter Assay Kit
The Parameter Corticosterone assay is a 3.5 hour competitive enzyme immunoassay designed to measure Corticosterone in cell culture supernates, serum, plasma, and urine.
Product Specifications
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
Biotin
Species
Multi-Species
Specificity
Corticosterone in human, mouse, and rat samples.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.24 | 3.98 | 8.05 | 1.39 | 4.08 | 8.21 |
| Standard Deviation | 0.078 | 0.179 | 0.605 | 0.099 | 0.241 | 0.462 |
| CV% | 6.3 | 4.5 | 7.5 | 7.1 | 5.9 | 5.6 |
Recovery for Corticosterone Parameter Assay Kit
The recovery of Corticosterone spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 114 | 107-124 |
| Human EDTA Plasma (n=4) | 108 | 87-127 |
| Human Heparin Plasma (n=4) | 107 | 97-122 |
| Human Serum (n=4) | 110 | 92-130 |
| Mouse EDTA Plasma (n=4) | 104 | 96-111 |
| Mouse Serum (n=4) | 96 | 79-113 |
| Rat EDTA Plasma (n=4) | 98 | 77-114 |
| Rat Serum (n=4) | 99 | 87-108 |
| Urine (n=4) | 105 | 92-121 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Corticosterone were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Corticosterone Parameter Assay Kit
Multi-species Corticosterone ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Corticosterone
Alternate Names
17-Deoxycortisol;C21H30O4
Additional Corticosterone Products
Product Documents for Corticosterone Parameter Assay Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Corticosterone Parameter Assay Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Corticosterone Parameter Assay Kit
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Protocols
View specific protocols for Corticosterone Parameter Assay Kit (KGE009):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Primary Antibody Solution to all wells except the non-specific binding (NSB) wells. Cover with a plate sealer, and incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
- Aspirate each well of the microplate and wash, repeating the process 3 times for a total of 4 washes.
- Add 100 µL of Pretreatment F to all wells.
- Add 50 µL of Standard, control, or sample to the appropriate wells.
- Add 50 µL of the Calibrator Diluent to the NSB and zero standard (B0) wells.
- Add 50 µL of Conjugate to all wells. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Primary Antibody Solution
100 µL Pretreatment F
50 µL Standard, Control, or Sample
50 µL of Calibrator Diluent
50 µL Conjugate
200 µL Substrate Solution
100 µL Stop Solution
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