Corticosterone Parameter Assay Kit

  (8 citations)     
Datasheet / CoA / SDS
Product Details
Assay Procedure
Citations (8)
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (10 uL)
  • Sensitivity
    0.047 ng/mL
  • Assay Range
    0.1 - 25 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Corticosterone in human, mouse, and rat samples.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Parameter Corticosterone assay is a 3.5 hour competitive enzyme immunoassay designed to measure Corticosterone in cell culture supernates, serum, plasma, and urine.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 1.24 3.98 8.05 1.39 4.08 8.21
Standard Deviation 0.078 0.179 0.605 0.099 0.241 0.462
CV% 6.3 4.5 7.5 7.1 5.9 5.6


The recovery of Corticosterone spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 114 107-124
Human EDTA Plasma (n=4) 108 87-127
Human Heparin Plasma (n=4) 107 97-122
Human Serum (n=4) 110 92-130
Mouse EDTA Plasma (n=4) 104 96-111
Mouse Serum (n=4) 96 79-113
Rat EDTA Plasma (n=4) 98 77-114
Rat Serum (n=4) 99 87-108
Urine (n=4) 105 92-121
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Corticosterone were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 Corticosterone [Biotin]
 Corticosterone [Biotin]
 Corticosterone [Biotin]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Corticosterone
Corticosterone, also known as Kendall's compound B and Reichstein substance B, is a glucocorticoid secreted by the adrenal gland cortex. It is produced in response to adrenal cortex stimulation by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Stress increases corticosteroid production. Corticosterone level is measured as an indicator of stress in patients with dietary restrictions, long-term memory impairment, and burn injuries.
  • Alternate Names:
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Primary Antibody Solution
  4.   Add 50 µL of Primary Antibody Solution to all wells except the non-specific binding (NSB) wells. Cover with a plate sealer, and incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
  5.   Aspirate each well of the microplate and wash, repeating the process 3 times for a total of 4 washes.

  6. 100 µL Pretreatment F
  7.   Add 100 µL of Pretreatment F to all wells.

  8. 50 µL Standard, Control, or Sample
  9.   Add 50 µL of Standard, control, or sample to the appropriate wells.

  10. 50 µL of Calibrator Diluent
  11.   Add 50 µL of the Calibrator Diluent to the NSB and zero standard (B0) wells.

  12. 50 µL Conjugate
  13.   Add 50 µL of Conjugate to all wells. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  14.   Aspirate and wash 4 times.

  15. 200 µL Substrate Solution
  16. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  17. 100 µL Stop Solution
  18. Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
Filter your results:

Sample Type
  1. The Anti-Stress Effect of Mentha arvensis in Immobilized Rats
    Authors: W Tian, MR Akanda, A Islam, HD Yang, SC Lee, JH Lee, SK Kim, YJ Choi, SY Im, BY Park
    Int J Mol Sci, 2018;19(2):.
    Species: Rat
    Sample Type: Serum
  2. Puerarin ameliorated the behavioral deficits induced by chronic stress in rats
    Authors: ZK Qiu, GH Zhang, DS Zhong, JL He, X Liu, JS Chen, DN Wei
    Sci Rep, 2017;7(1):6266.
    Species: Rat
    Sample Type: Serum
  3. Impaired Spinal Glucocorticoid Receptor Signaling Contributes to the Attenuating Effect of Depression on Mechanical Allodynia and Thermal Hyperalgesia in Rats with Neuropathic Pain
    Authors: X Wei, Y Sun, F Luo
    Front Cell Neurosci, 2017;11(0):145.
    Species: Rat
    Sample Type: Serum
  4. Physiological stress-induced corticosterone increases heme uptake via KLF4-HCP1 signaling pathway in hippocampus neurons
    Authors: H Li, C Zhang, H Shen, Z Shen, L Wu, F Mo, M Li
    Sci Rep, 2017;7(1):5745.
    Species: Rat
    Sample Type: Serum
  5. Melatonin ameliorates restraint stress-induced oxidative stress and apoptosis in testicular cells via NF-?B/iNOS and Nrf2/ HO-1 signaling pathway
    Authors: Y Guo, J Sun, T Li, Q Zhang, S Bu, Q Wang, D Lai
    Sci Rep, 2017;7(1):9599.
    Species: Mouse
    Sample Type: Serum
  6. Stellate ganglion block attenuates chronic stress induced depression in rats
    Authors: W Wang, W Shi, H Qian, X Deng, T Wang, W Li
    PLoS ONE, 2017;12(8):e0183995.
    Species: Rat
    Sample Type: Plasma
  7. Ghrelin-induced food intake and adiposity depend on central mTORC1/S6K1 signaling.
    Authors: Stevanovic D, Trajkovic V, Muller-Luhlhoff S, Brandt E, Abplanalp W, Bumke-Vogt C, Liehl B, Wiedmer P, Janjetovic K, Starcevic V, Pfeiffer A, Al-Hasani H, Tschop M, Castaneda T
    Mol Cell Endocrinol, 2013;381(1):280-90.
    Species: Rat
    Sample Type: Serum
  8. Antidepressant-like effects of the ethyl acetate soluble fraction of the root bark of Morus alba on the immobility behavior of rats in the forced swim test.
    Authors: Lim, Dong Woo, Kim, Yun Tai, Park, Ji-Hae, Baek, Nam-In, Han, Daeseok
    Molecules, 0;19(6):7981-9.
    Species: Rat
    Sample Type: Serum


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