Corticosterone Parameter Assay Kit

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KGE009
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Multi-species Corticosterone ELISA Standard Curve
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Corticosterone Parameter Assay Kit Summary

Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (10 uL)
Sensitivity
0.047 ng/mL
Assay Range
0.1 - 25 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Corticosterone in human, mouse, and rat samples.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Parameter Corticosterone assay is a 3.5 hour competitive enzyme immunoassay designed to measure Corticosterone in cell culture supernates, serum, plasma, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.24 3.98 8.05 1.39 4.08 8.21
Standard Deviation 0.078 0.179 0.605 0.099 0.241 0.462
CV% 6.3 4.5 7.5 7.1 5.9 5.6

Recovery

The recovery of Corticosterone spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 114 107-124
Human EDTA Plasma (n=4) 108 87-127
Human Heparin Plasma (n=4) 107 97-122
Human Serum (n=4) 110 92-130
Mouse EDTA Plasma (n=4) 104 96-111
Mouse Serum (n=4) 96 79-113
Rat EDTA Plasma (n=4) 98 77-114
Rat Serum (n=4) 99 87-108
Urine (n=4) 105 92-121

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Corticosterone were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Multi-species Corticosterone ELISA Linearity
Multi-species Corticosterone ELISA Linearity
Multi-species Corticosterone ELISA Linearity

Scientific Data

Multi-species Corticosterone ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Corticosterone

Corticosterone, also known as Kendall's compound B and Reichstein substance B, is a glucocorticoid secreted by the adrenal gland cortex. It is produced in response to adrenal cortex stimulation by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Stress increases corticosteroid production. Corticosterone level is measured as an indicator of stress in patients with dietary restrictions, long-term memory impairment, and burn injuries.

Alternate Names:
17-Deoxycortisol;C21H30O4; Corticosterone
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Primary Antibody Solution
  4.   Add 50 µL of Primary Antibody Solution to all wells except the non-specific binding (NSB) wells. Cover with a plate sealer, and incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
  5.   Aspirate each well of the microplate and wash, repeating the process 3 times for a total of 4 washes.

  6. 100 µL Pretreatment F
  7.   Add 100 µL of Pretreatment F to all wells.

  8. 50 µL Standard, Control, or Sample
  9.   Add 50 µL of Standard, control, or sample to the appropriate wells.

  10. 50 µL of Calibrator Diluent
  11.   Add 50 µL of the Calibrator Diluent to the NSB and zero standard (B0) wells.

  12. 50 µL Conjugate
  13.   Add 50 µL of Conjugate to all wells. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  14.   Aspirate and wash 4 times.

  15. 200 µL Substrate Solution
  16. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  17. 100 µL Stop Solution
  18. Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Corticosterone Parameter Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

46 Citations: Showing 1 - 10
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  1. Stress-induced red nucleus attenuation induces anxiety-like behavior and lymph node CCL5 secretion
    Authors: Shi, DD;Zhang, YD;Zhang, S;Liao, BB;Chu, MY;Su, S;Zhuo, K;Hu, H;Zhang, C;Wang, Z;
    Nature communications
    Species: Rat
    Sample Types: Serum
  2. Hepatic-Specific FGF21 Knockout Abrogates Ovariectomy-Induced Obesity by Reversing Corticosterone Production
    Authors: Xu, J;Shao, X;Zeng, H;Wang, C;Li, J;Peng, X;Zhuo, Y;Hua, L;Meng, F;Han, X;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Serum
  3. Not so fast: Paradoxically increased variability in the glucose tolerance test due to food withdrawal in continuous glucose-monitored mice
    Authors: Rubio, WB;Cortopassi, MD;Ramachandran, D;Walker, SJ;Balough, EM;Wang, J;Banks, AS;
    Molecular metabolism
    Species: Mouse
    Sample Types: Serum, Urine
  4. Interplay between Learning and Voluntary Wheel Running in Male C57BL/6NCrl Mice
    Authors: L Niiranen, V Stenbäck, M Tulppo, KH Herzig, KA Mäkelä
    International Journal of Molecular Sciences, 2023-02-21;24(5):.
    Species: Mouse
    Sample Types: Urine
  5. Mechanic Insight into the Distinct and Common Roles of Ovariectomy Versus Adrenalectomy on Adipose Tissue Remodeling in Female Mice
    Authors: W Chen, F Meng, X Zeng, X Cao, G Bu, X Du, G Yu, F Kong, Y Li, T Gan, X Han
    International Journal of Molecular Sciences, 2023-01-24;24(3):.
    Species: Mouse
    Sample Types: Serum
  6. A paraventricular thalamus to central amygdala neural circuit modulates acute stress-induced heightened wakefulness
    Authors: J Zhao, C Liu, F Zhang, Z Zheng, F Luo, J Xia, Y Wang, Z Zhang, J Tang, Z Song, S Li, K Xu, M Chen, C Jiang, C He, L Tang, Z Hu, D Gao, S Ren
    Cell Reports, 2022-12-13;41(11):111824.
    Species: Mouse
    Sample Types: Plasma
  7. Divergent neurocircuitry dissociates two components of the stress response: glucose mobilization and anxiety-like behavior
    Authors: X Jia, S Chen, X Li, S Tao, J Lai, H Liu, K Huang, Y Tian, P Wei, F Yang, Z Lu, Z Chen, XA Liu, F Xu, L Wang
    Cell Reports, 2022-11-08;41(6):111586.
    Species: Transgenic Mouse
    Sample Types: Serum
  8. Advanced age is associated with changes in alveolar macrophages and their responses to the stress of traumatic injury
    Authors: DM Boe, HJ Hulsebus, KM Najarro, JE Mullen, H Kim, AC Tan, RH McMahan, EJ Kovacs
    Journal of leukocyte biology, 2022-09-19;0(0):.
    Species: Mouse
    Sample Types: Serum
  9. 11beta-Hydroxysteroid Dehydrogenase Type 1 within Osteoclasts Mediates the Bone Protective Properties of Therapeutic Corticosteroids in Chronic Inflammation
    Authors: CG Fenton, A Crastin, CS Martin, S Suresh, I Montagna, B Hussain, AJ Naylor, SW Jones, MS Hansen, CM Gorvin, M Price, A Filer, MS Cooper, GG Lavery, K Raza, RS Hardy
    International Journal of Molecular Sciences, 2022-06-30;23(13):.
    Species: Mouse
    Sample Types: Serum
  10. Heat-inactivated Lacticaseibacillus paracasei N1115 alleviates the damage due to brain function caused by long-term antibiotic cocktail exposure in mice
    Authors: Y Zhang, H Liang, Y Wang, R Cheng, F Pu, Y Yang, J Li, S Wu, X Shen, F He
    Bmc Neuroscience, 2022-06-26;23(1):38.
    Species: Mouse
    Sample Types: Serum
  11. Unravelling the sex-specific diversity and functions of adrenal gland macrophages
    Authors: B Dolfi, A Gallerand, MM Firulyova, Y Xu, J Merlin, A Dumont, A Castiglion, N Vaillant, S Quemener, H Gerke, MI Stunault, PR Schrank, SH Kim, A Zhu, J Ding, J Gilleron, V Magnone, P Barbry, D Dombrowicz, C Duranton, A Wakkach, C Blin-Wakka, B Becher, S Pagnotta, RJ Argüello, P Rantakari, S Chakarov, F Ginhoux, K Zaitsev, KW Kim, L Yvan-Charv, RR Guinamard, JW Williams, S Ivanov
    Cell Reports, 2022-06-14;39(11):110949.
    Species: Mouse
    Sample Types: Serum, Tissue Homogenates
  12. The Alleviation of Gut Microbiota-Induced Depression and Colitis in Mice by Anti-Inflammatory Probiotics NK151, NK173, and NK175
    Authors: JW Yoo, YJ Shin, X Ma, YH Son, HM Jang, CK Lee, DH Kim
    Nutrients, 2022-05-16;14(10):.
    Species: Mouse
    Sample Types: Tissue Lysates
  13. Multi-Probiotics ameliorate Major depressive disorder and accompanying gastrointestinal syndromes via serotonergic system regulation
    Authors: P Tian, R Zou, L Wang, Y Chen, X Qian, J Zhao, H Zhang, L Qian, Q Wang, G Wang, W Chen
    Journal of advanced research, 2022-05-11;0(0):.
    Species: Mouse
    Sample Types: Serum
  14. Sex differences and heritability of adrenal steroidogenesis in offspring rats induced by prenatal nicotine exposure
    Authors: Y Chen, F Duan, L Liu, G Chen, Z He, H Huang, H Wang
    The Journal of steroid biochemistry and molecular biology, 2022-03-31;221(0):106102.
    Species: Rat
    Sample Types: Cell Culture Supernates
  15. Astragaloside IV ameliorates peripheral immunosuppression induced by cerebral ischemia through inhibiting HPA axis
    Authors: Y Zou, S Li, T Chen, Z Li, X Gao, Z Wang
    International immunopharmacology, 2022-01-25;105(0):108569.
    Species: Mouse
    Sample Types: Serum
  16. Chronic Systemic Dexamethasone Regulates the Mineralocorticoid/Glucocorticoid Pathways Balance in Rat Ocular Tissues
    Authors: M Zola, D Mejlachowi, R Gregorio, MC Naud, F Jaisser, M Zhao, F Behar-Cohe
    International Journal of Molecular Sciences, 2022-01-24;23(3):.
    Species: Rat
    Sample Types: Serum
  17. CRF-receptor1 modulation of the dopamine projection to prelimbic cortex facilitates cognitive flexibility after acute and chronic stress
    Authors: M David, B Serena, B Jeremy, T Madeline, BW Bernard
    Neurobiology of stress, 2021-12-24;16(0):100424.
    Species: Rat
    Sample Types: Serum
  18. Dysfunction of the hypothalamic?pituitary?adrenal axis in male rat offspring with prenatal food restriction: Fetal programming of hypothalamic hyperexcitability and poor hippocampal feedback
    Authors: Y Wen, S Cheng, J Lu, X He, Z Jiao, D Xu, H Wang
    Molecular Medicine Reports, 2021-11-19;25(1):.
    Species: Rat
    Sample Types: Serum
  19. Psychological stress impairs IL22-driven protective gut mucosal immunity against colonising pathobionts
    Authors: CR Shaler, AA Parco, W Elhenawy, J Dourka, J Jury, EF Verdu, BK Coombes
    Nature Communications, 2021-11-18;12(1):6664.
    Species: Mouse
    Sample Types: Serum
  20. Low glycaemic diets alter lipid metabolism to influence tumour growth
    Authors: EC Lien, AM Westermark, Y Zhang, C Yuan, Z Li, AN Lau, KM Sapp, BM Wolpin, MG Vander Hei
    Nature, 2021-10-20;0(0):.
    Species: Human
    Sample Types: Plasma
  21. Predictable maternal separation confers adult stress resilience via the medial prefrontal cortex oxytocin signaling pathway in rats
    Authors: DD Shi, YD Zhang, YY Ren, SY Peng, TF Yuan, Z Wang
    Molecular Psychiatry, 2021-09-24;0(0):.
    Species: Rat
    Sample Types: Serum
  22. Mice with mutations in Trpm1, a gene in the locus of 15q13.3 microdeletion syndrome, display pronounced hyperactivity and decreased anxiety-like behavior
    Authors: T Hori, S Ikuta, S Hattori, K Takao, T Miyakawa, C Koike
    Molecular Brain, 2021-03-30;14(1):61.
    Species: Mouse
    Sample Types: Serum
  23. Diet and companionship modulate pain via a serotonergic mechanism
    Authors: H Tran, V Sagi, S Jarrett, EF Palzer, RD Badgaiyan, K Gupta
    Scientific Reports, 2021-02-01;11(1):2330.
    Species: Mouse
    Sample Types: Serum
  24. Effects of age and social isolation on murine hippocampal biochemistry and behavior
    Authors: A Panossian, MW Cave, BA Patel, EL Brooks, MS Flint, MS Yeoman
    Mech. Ageing Dev., 2020-08-28;0(0):111337.
    Species: Mouse
    Sample Types: Tissue Homogenates
  25. Electro-acupuncture regulates glucose metabolism in chronic stress model rats
    Authors: FQ Ma, CJ Sun, JJ Wei, YD Wang, JC Shen, JJ Chang
    Sci Rep, 2020-07-09;10(1):11281.
    Species: Rat
    Sample Types: Serum
  26. Synchronization of the circadian clock by time-restricted feeding with progressive increasing calorie intake. Resemblances and differences regarding a sustained hypocaloric restriction
    Authors: AC García-Gay, M Miranda-An, I Turrubiate, L López-De P, GN Bocanegra-, A López-Isla, M Díaz-Muñoz, I Méndez
    Sci Rep, 2020-06-22;10(1):10036.
    Species: Rat
    Sample Types: Serum
  27. The Role of DNMT and HDACs in the Fetal Programming of Hypertension by Glucocorticoids
    Authors: J Lamothe, S Khurana, S Tharmaling, C Williamson, CJ Byrne, N Khaper, S Mercier, TC Tai
    Oxid Med Cell Longev, 2020-03-28;2020(0):5751768.
    Species: Rat
    Sample Types: Plasma
  28. Hypothalamus-pituitary-adrenal axis imbalance and inflammation contribute to sex differences in separation- and restraint-induced depression
    Authors: Y Dong, X Wang, Y Zhou, Q Zheng, Z Chen, H Zhang, Z Sun, G Xu, G Hu
    Horm Behav, 2020-03-16;122(0):104741.
    Species: Mouse
    Sample Types: Serum
  29. Relationships between glucocorticoids and infection with Batrachochytrium dendrobatidis in three amphibian species
    Authors: TT Hammond, PE Blackwood, SA Shablin, CL Richards-Z
    Gen. Comp. Endocrinol., 2019-09-04;285(0):113269.
    Species: Frog
    Sample Types: Tissue Culture Supernates
  30. Androgens augment pulmonary responses to ozone in mice
    Authors: RS Osgood, DI Kasahara, H Tashiro, Y Cho, SA Shore
    Physiol Rep, 2019-09-01;7(18):e14214.
    Species: Mouse
    Sample Types: Serum
  31. Critical Role of Regulatory T Cells in the Latency and Stress-Induced Reactivation of HSV-1
    Authors: W Yu, S Geng, Y Suo, X Wei, Q Cai, B Wu, X Zhou, Y Shi, B Wang
    Cell Rep, 2018-11-27;25(9):2379-2389.e3.
    Species: Mouse
    Sample Types: Plasma
  32. Baicalin Exerts Anti-Airway Inflammation and Anti-Remodelling Effects in Severe Stage Rat Model of Chronic Obstructive Pulmonary Disease
    Authors: G Wang, N Mohammadtu, Y Lv, H Zhang, J Sun, J Dong
    Evid Based Complement Alternat Med, 2018-10-08;2018(0):7591348.
    Species: Rat
    Sample Types: Serum
  33. Ablation of somatostatin cells leads to impaired pancreatic islet function and neonatal death in rodents
    Authors: N Li, Z Yang, Q Li, Z Yu, X Chen, JC Li, B Li, SL Ning, M Cui, JP Sun, X Yu
    Cell Death Dis, 2018-06-07;9(6):682.
    Species: Rat
    Sample Types: Plasma
  34. Programming for increased expression of hippocampal GAD67 mediated the hypersensitivity of the hypothalamic-pituitary-adrenal axis in male offspring rats with prenatal ethanol exposure
    Authors: J Lu, Z Jiao, Y Yu, C Zhang, X He, Q Li, D Xu, H Wang
    Cell Death Dis, 2018-05-31;9(6):659.
    Species: Rat
    Sample Types: Serum
  35. Effect of pre-slaughter shackling and wing flapping on plasma parameters, postmortem metabolism, AMPK, and meat quality of broilers
    Authors: JC Huang, J Yang, M Huang, ZS Zhu, XB Sun, BH Zhang, XL Xu, WG Meng, KJ Chen, BC Xu
    Poult. Sci., 2018-05-01;0(0):.
    Species: Chicken
    Sample Types: Plasma
  36. Psychosocial stress delays recovery of postoperative pain following incisional surgery in the rat
    Authors: V Arora, TJ Martin, CA Aschenbren, K Hayashida, SA Kim, RA Parker, JC Eisenach, CM Peters
    Neuroscience, 2018-04-22;0(0):.
    Species: Rat
    Sample Types: Plasma
  37. The Anti-Stress Effect of Mentha arvensis in Immobilized Rats
    Authors: W Tian, MR Akanda, A Islam, HD Yang, SC Lee, JH Lee, SK Kim, YJ Choi, SY Im, BY Park
    Int J Mol Sci, 2018-01-25;19(2):.
    Species: Rat
    Sample Types: Serum
  38. Stellate ganglion block attenuates chronic stress induced depression in rats
    Authors: W Wang, W Shi, H Qian, X Deng, T Wang, W Li
    PLoS ONE, 2017-08-31;12(8):e0183995.
    Species: Rat
    Sample Types: Plasma
  39. Melatonin ameliorates restraint stress-induced oxidative stress and apoptosis in testicular cells via NF-?B/iNOS and Nrf2/ HO-1 signaling pathway
    Authors: Y Guo, J Sun, T Li, Q Zhang, S Bu, Q Wang, D Lai
    Sci Rep, 2017-08-29;7(1):9599.
    Species: Mouse
    Sample Types: Serum
  40. Puerarin ameliorated the behavioral deficits induced by chronic stress in rats
    Authors: ZK Qiu, GH Zhang, DS Zhong, JL He, X Liu, JS Chen, DN Wei
    Sci Rep, 2017-07-24;7(1):6266.
    Species: Rat
    Sample Types: Serum
  41. Physiological stress-induced corticosterone increases heme uptake via KLF4-HCP1 signaling pathway in hippocampus neurons
    Authors: H Li, C Zhang, H Shen, Z Shen, L Wu, F Mo, M Li
    Sci Rep, 2017-07-18;7(1):5745.
    Species: Rat
    Sample Types: Serum
  42. Impaired Spinal Glucocorticoid Receptor Signaling Contributes to the Attenuating Effect of Depression on Mechanical Allodynia and Thermal Hyperalgesia in Rats with Neuropathic Pain
    Authors: X Wei, Y Sun, F Luo
    Front Cell Neurosci, 2017-05-19;11(0):145.
    Species: Rat
    Sample Types: Serum
  43. Impact of different analgesic depths and abdominal trauma of different severities on stress and recovery of rats undergoing total intravenous anesthesia
    Authors: HM Huang, J Cao, LM Zhu, YQ Chen, FD Lu, HW Cai
    J Pain Res, 2017-05-12;10(0):1143-1153.
    Species: Rat
    Sample Types: Plasma
  44. Antidepressant-like effects of the ethyl acetate soluble fraction of the root bark of Morus alba on the immobility behavior of rats in the forced swim test.
    Authors: Lim, Dong Woo, Kim, Yun Tai, Park, Ji-Hae, Baek, Nam-In, Han, Daeseok
    Molecules, 2014-06-12;19(6):7981-9.
    Species: Rat
    Sample Types: Serum
  45. Ghrelin-induced food intake and adiposity depend on central mTORC1/S6K1 signaling.
    Authors: Stevanovic D, Trajkovic V, Muller-Luhlhoff S, Brandt E, Abplanalp W, Bumke-Vogt C, Liehl B, Wiedmer P, Janjetovic K, Starcevic V, Pfeiffer A, Al-Hasani H, Tschop M, Castaneda T
    Mol Cell Endocrinol, 2013-08-29;381(1):280-90.
    Species: Rat
    Sample Types: Serum

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