• Format
    96-well strip plate
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (10 uL)
  • Sensitivity
    0.047 ng/mL
  • Assay Range
    0.1 - 25 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Corticosterone in human, mouse, and rat samples.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Parameter Corticosterone assay is a 3.5 hour competitive enzyme immunoassay designed to measure Corticosterone in cell culture supernates, serum, plasma, and urine.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.0780.1790.6050.0990.2410.462


The recovery of Corticosterone spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 114 107-124
Human EDTA Plasma (n=4) 108 87-127
Human Heparin Plasma (n=4) 107 97-122
Human Serum (n=4) 110 92-130
Mouse EDTA Plasma (n=4) 104 96-111
Mouse Serum (n=4) 96 79-113
Rat EDTA Plasma (n=4) 98 77-114
Rat Serum (n=4) 99 87-108
Urine (n=4) 105 92-121
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Corticosterone were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Corticosterone Parameter Assay Kit
Corticosterone Parameter Assay Kit
Corticosterone Parameter Assay Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Corticosterone
Corticosterone, also known as Kendall's compound B and Reichstein substance B, is a glucocorticoid secreted by the adrenal gland cortex. It is produced in response to adrenal cortex stimulation by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Stress increases corticosteroid production. Corticosterone level is measured as an indicator of stress in patients with dietary restrictions, long-term memory impairment, and burn injuries.
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Primary Antibody Solution
    4.   Add 50 µL of Primary Antibody Solution to all wells except the non-specific binding (NSB) wells. Cover with a plate sealer, and incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
    5.   Aspirate each well of the microplate and wash, repeating the process 3 times for a total of 4 washes.

    6. 100 µL Pretreatment F
    7.   Add 100 µL of Pretreatment F to all wells.

    8. 50 µL Standard, Control, or Sample
    9.   Add 50 µL of Standard, control, or sample to the appropriate wells.

    10. 50 µL of Calibrator Diluent
    11.   Add 50 µL of the Calibrator Diluent to the NSB and zero standard (B0) wells.

    12. 50 µL Conjugate
    13.   Add 50 µL of Conjugate to all wells. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    14.   Aspirate and wash 4 times.

    15. 200 µL Substrate Solution
    16. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    17. 100 µL Stop Solution
    18. Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 1 of 1

    1. Ghrelin-induced food intake and adiposity depend on central mTORC1/S6K1 signaling.
      Authors: Stevanovic D, Trajkovic V, Muller-Luhlhoff S, Brandt E, Abplanalp W, Bumke-Vogt C, Liehl B, Wiedmer P, Janjetovic K, Starcevic V, Pfeiffer A, Al-Hasani H, Tschop M, Castaneda T
      Mol Cell Endocrinol, 2013;381(1):280-90.
      Species: Rat
      Sample Type: Serum

    Have you used Corticosterone Parameter Assay Kit?

    Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Order Details

    Contact Information

    Certificate of Analysis Lookup

    The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

    Certificate of Analysis Lookup

    Research Areas of Interest
    R&D Systems Guarantee


    Customer Information