< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
The Parameter Corticosterone assay is a 3.5 hour competitive enzyme immunoassay designed to measure Corticosterone in cell culture supernates, serum, plasma, and urine.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
The recovery of Corticosterone spiked to levels throughout the range of the assay in various matrices was evaluated.
Average % Recovery
Cell Culture Media (n=4)
Human EDTA Plasma (n=4)
Human Heparin Plasma (n=4)
Human Serum (n=4)
Mouse EDTA Plasma (n=4)
Mouse Serum (n=4)
Rat EDTA Plasma (n=4)
Rat Serum (n=4)
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Corticosterone were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Corticosterone, also known as Kendall's compound B and Reichstein substance B, is a glucocorticoid secreted by the adrenal gland cortex. It is produced in response to adrenal cortex stimulation by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Stress increases corticosteroid production. Corticosterone level is measured as an indicator of stress in patients with dietary restrictions, long-term memory impairment, and burn injuries.
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
Prepare all reagents, standard dilutions, and samples as directed in the product insert.
Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
50 µL Primary Antibody Solution
Add 50 µL of Primary Antibody Solution to all wells except the non-specific binding (NSB) wells. Cover with a plate sealer, and incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
Aspirate each well of the microplate and wash, repeating the process 3 times for a total of 4 washes.
100 µL Pretreatment F
Add 100 µL of Pretreatment F to all wells.
50 µL Standard, Control, or Sample
Add 50 µL of Standard, control, or sample to the appropriate wells.
50 µL of Calibrator Diluent
Add 50 µL of the Calibrator Diluent to the NSB and zero standard (B0) wells.
50 µL Conjugate
Add 50 µL of Conjugate to all wells. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
Aspirate and wash 4 times.
200 µL Substrate Solution
Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
100 µL Stop Solution
Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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