Cortisol Parameter Assay Kit

  (33 citations)     
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Citations (33)
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  • Assay Length
    2.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (10 uL), Serum (10 uL), Saliva (20 uL), Urine (10 uL)
  • Sensitivity
    0.111 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Saliva, Urine)
  • Specificity
    Measures Cortisol levels in various samples
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC68 , Parameter Immunoassay Control Set 901 for Cortisol - Please Inquire
Product Summary
The Parameter Cortisol Immunoassay is a 2.5 hour competitive enzyme immunoassay designed to measure Cortisol in cell culture supernates, serum, saliva, and urine.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Saliva, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 1.3 4.5 6.51 1.11 3.42 5.54
Standard Deviation 0.12 0.241 0.411 0.235 0.319 0.576
CV% 9.2 5.4 6.3 21.2 9.3 10.4

Recovery

The recovery of Cortisol spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 87-113
Saliva (n=4) 101 80-118
Serum (n=4) 104 92-114
Urine (n=4) 92 78-113
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Cortisol were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 Cortisol [Biotin]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Cortisol
Cortisol, also known as hydrocortisone or compound F, is the primary glucocorticoid produced and secreted by the adrenal cortex. Found either free or bound to corticosteroid-binding globulin (CBG) in the blood, cortisol is involved in glucose homeostasis, inflammation, hypersensitivity, immunosuppression, and disease resistance. Plasma cortisol levels increase in response to stress and abnormal levels are implicated in prostate cancer, depression, and schizophrenia. Cushing's disease is caused by systemic cortisol excess.
  • Alternate Names:
    Compound F; Cortisol; Hydrocortisone
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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Species
Sample Type
  1. Effect of speed endurance training and reduced training volume on running economy and single muscle fiber adaptations in trained runners
    Authors: C Skovgaard, D Christians, PM Christense, NW Almquist, M Thomassen, J Bangsbo
    Physiol Rep, 2018;6(3):.
    Species: Human
    Sample Type: Plasma
  2. Psychological Stress Deteriorates Skin Barrier Function by Activating 11?-Hydroxysteroid Dehydrogenase 1 and the HPA Axis
    Authors: SJ Choe, D Kim, EJ Kim, JS Ahn, EJ Choi, ED Son, TR Lee, EH Choi
    Sci Rep, 2018;8(1):6334.
    Species: Human
    Sample Type: Cell Culture Supernates
  3. Relaxation - Induced by Vibroacoustic Stimulation via a Body Monochord and via Relaxation Music - Is Associated with a Decrease in Tonic Electrodermal Activity and an Increase of the Salivary Cortisol Level in Patients with Psychosomatic Disorders
    Authors: H Sandler, U Fendel, P Buáe, M Rose, R B”sel, BF Klapp
    PLoS ONE, 2017;12(1):e0170411.
    Species: Human
    Sample Type: Saliva
  4. Glucocorticoids modulate human brown adipose tissue thermogenesis in vivo
    Authors: H Scotney, ME Symonds, J Law, H Budge, D Sharkey, KN Manolopoul
    Metab. Clin. Exp., 2017;70(0):125-132.
    Species: Human
    Sample Type: Plasma
  5. Ramadan model of intermittent fasting for 28�d had no major effect on body composition, glucose metabolism, or cognitive functions in healthy lean men
    Authors: NM Harder-Lau, A Rosenberg, FB Benatti, JA Damm, C Thomsen, EL Mortensen, BK Pedersen, R Krogh-Mads
    Nutrition, 2017;37(0):92-103.
    Species: Human
    Sample Type: Serum
  6. Transcriptomic analyses and leukocyte telomere length measurement in subjects exposed to severe recent stressful life events
    Authors: N Lopizzo, S Tosato, V Begni, S Tomassi, N Cattane, M Barcella, G Turco, M Ruggeri, MA Riva, CM Pariante, A Cattaneo
    Transl Psychiatry, 2017;7(2):e1042.
    Species: Human
    Sample Type: Serum
  7. Topical mevastatin promotes wound healing by inhibiting the transcription factor c-Myc via the glucocorticoid receptor and the long noncoding RNA Gas5
    Authors: AP Sawaya, I Pastar, O Stojadinov, S Lazovic, SC Davis, J Gil, RS Kirsner, M Tomic-Cani
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
  8. Chronic grouped social restriction triggers long-lasting immune system adaptations
    Authors: R Tian, G Hou, L Song, J Zhang, TF Yuan
    Oncotarget, 2017;8(20):33652-33657.
    Species: Human
    Sample Type: Serum
  9. Beneficial effect of combined treatment with octreotide and pasireotide in PCK rats, an orthologous model of human autosomal recessive polycystic kidney disease
    Authors: M Kugita, K Nishii, T Yamaguchi, A Suzuki, Y Yuzawa, S Horie, E Higashihar, S Nagao
    PLoS ONE, 2017;12(5):e0177934.
    Species: Rat
    Sample Type: Serum
  10. Flavour-enhanced cortisol release during gum chewing
    Authors: Y Hasegawa, Y Tachibana, T Ono, H Kishimoto
    PLoS ONE, 2017;12(4):e0173475.
    Species: Human
    Sample Type: Saliva
  11. Long-term Levodopa Treatment Accelerates the Circadian Rhythm Dysfunction in a 6-hydroxydopamine Rat Model of Parkinson's Disease
    Authors: SY Li, YL Wang, WW Liu, DJ Lyu, F Wang, CJ Mao, YP Yang, LF Hu, CF Liu
    Chin. Med. J., 2017;130(9):1085-1092.
    Species: Rat
    Sample Type: Plasma
  12. Intense Exercise Promotes Adult Hippocampal Neurogenesis But Not Spatial Discrimination
    Authors: JH So, C Huang, M Ge, G Cai, L Zhang, Y Lu, Y Mu
    Front Cell Neurosci, 2017;11(0):13.
    Species: Mouse
    Sample Type: Serum
  13. Novel 11?-hydroxysteroid dehydrogenase 1 inhibitors reduce cortisol levels in keratinocytes and improve dermal collagen content in human ex vivo skin after exposure to cortisone and UV
    Authors: SM Boudon, A Vuorinen, P Geotti-Bia, E Wandeler, DV Kratschmar, M Heidl, R Campiche, E Jackson, A Odermatt
    PLoS ONE, 2017;12(2):e0171079.
    Species: Human
    Sample Type: Cell Culture Supernates
  14. Tumor-Induced IL-6 Reprograms Host Metabolism to Suppress Anti-tumor Immunity
    Authors: Douglas T Fearon
    Cell Metab., 2016;24(5):672-684.
    Species: Human
    Sample Type: Serum
  15. Effect of Peripheral Electrical Stimulation (PES) on Nocturnal Blood Glucose in Type 2 Diabetes: A Randomized Crossover Pilot Study
    PLoS ONE, 2016;11(12):e0168805.
    Species: Human
    Sample Type: Serum
  16. Anti-Inflammatory Modulation of Microglia via CD163-Targeted Glucocorticoids Protects Dopaminergic Neurons in the 6-OHDA Parkinson's Disease Model
    J Neurosci, 2016;36(36):9375-90.
  17. The Differential Hormonal Milieu of Morning versus Evening May Have an Impact on Muscle Hypertrophic Potential
    PLoS ONE, 2016;11(9):e0161500.
    Species: Human
    Sample Type: Serum
  18. Circadian variations in clinical symptoms and concentrations of inflammatory cytokines, melatonin, and cortisol in polymyalgia rheumatica before and during prednisolone treatment: a controlled, observational, clinical experimental study
    Arthritis Res Ther, 2016;18(1):174.
    Species: Human
    Sample Type: Plasma
  19. Urinary cortisol and depression in early pregnancy: role of adiposity and race.
    Authors: Luiza J, Gallaher M, Powers R
    BMC Pregnancy Childbirth, 2015;15(0):30.
    Species: Human
    Sample Type: Plasma
  20. Metabolic implications when employing heavy pre- and post-exercise rapid-acting insulin reductions to prevent hypoglycaemia in type 1 diabetes patients: a randomised clinical trial.
    Authors: Campbell M, Walker M, Trenell M, Luzio S, Dunseath G, Tuner D, Bracken R, Bain S, Russell M, Stevenson E, West D
    PLoS ONE, 2014;9(5):e97143.
    Species: Human
    Sample Type: Serum
  21. Comparative transcriptomics of Atlantic Salmo salar, chum Oncorhynchus keta and pink salmon O. gorbuscha during infections with salmon lice Lepeophtheirus salmonis.
    Authors: Sutherland, Ben J G, Koczka, Kim W, Yasuike, Motoshig, Jantzen, Stuart G, Yazawa, Ryosuke, Koop, Ben F, Jones, Simon R
    BMC Genomics, 2014;15(0):200.
    Species: Fish - Salmon
    Sample Type: Plasma
  22. Hyperglycemia is associated with enhanced gluconeogenesis in a rat model of permanent cerebral ischemia.
    Authors: Wang Y, Chen C, Lin S, Chuang Y, Sheu W, Tung K
    Mol Cell Endocrinol, 2014;367(1):50-6.
    Species: Rat
    Sample Type: Plasma
  23. Placental P-glycoprotein is unaffected by timing of antenatal glucocorticoid therapy but reduced in SGA preterm infants.
    Authors: Hodyl N, Stark M, Butler M, Clifton V
    Placenta, 2013;34(4):325-30.
    Species: Human
    Sample Type: Serum
  24. Involvement of GR and p300 in the induction of H6PD by cortisol in human amnion fibroblasts.
    Authors: Wang W, Guo C, Li W, Li J, Wang W, Myatt L, Sun K
    Endocrinology, 2012;153(12):5993-6002.
    Species: Human
    Sample Type: Cell Culture Supernates
  25. Is there a morning-to-evening difference in the acute IL-6 and cortisol responses to resistance exercise?
    Authors: Pledge D, Grosset JF, Onambele-Pearson GL
    Cytokine, 2011;55(2):318-23.
    Species: Human
    Sample Type: Serum
  26. Airway expression of the epithelial sodium channel alpha-subunit correlates with cortisol in term newborns.
    Authors: Janer C, Pitkanen OM, Helve O, Andersson S
    Pediatrics, 2011;128(2):e414-21.
    Species: Human
    Sample Type: Saliva
  27. Steroid synthesis by primary human keratinocytes; implications for skin disease.
    Authors: Hannen RF, Michael AE, Jaulim A, Bhogal R, Burrin JM, Philpott MP
    Biochem. Biophys. Res. Commun., 2011;404(1):62-7.
    Species: Human
    Sample Type: Cell Culture Supernates
  28. Cortisol Synthesis in Epidermis Is Induced by IL-1 and Tissue Injury.
    Authors: Vukelic S, Stojadinovic O, Pastar I, Rabach M, Krzyzanowska A, Lebrun E, Davis SC, Resnik S, Brem H, Tomic-Canic M
    J. Biol. Chem., 2011;286(12):10265-75.
    Species: Human
    Sample Type: Cell Culture Supernates
  29. Immune perturbations in patients along the perioperative period: alterations in cell surface markers and leukocyte subtypes before and after surgery.
    Authors: Bartal I, Melamed R, Greenfeld K, Atzil S, Glasner A, Domankevich V, Naor R, Beilin B, Yardeni IZ, Ben-Eliyahu S
    Brain Behav. Immun., 2010;24(3):376-86.
    Species: Human
    Sample Type: Plasma
  30. Skeletal muscle myofibrillar and sarcoplasmic protein synthesis rates are affected differently by altitude-induced hypoxia in native lowlanders.
    Authors: Holm L, Haslund ML, Robach P, van Hall G, Calbet JA, Saltin B, Lundby C
    PLoS ONE, 2010;5(12):e15606.
    Species: Human
    Sample Type: Plasma
  31. Effects of exenatide on circulating glucose, insulin, glucagon, cortisol and catecholamines in healthy volunteers during exercise.
    Authors: Khoo EY, Wallis J, Tsintzas K, Macdonald IA, Mansell P
    Diabetologia, 2010;53(1):139-43.
    Species: Human
    Sample Type: Serum
  32. Th2 cytokine-induced upregulation of 11beta-hydroxysteroid dehydrogenase-1 facilitates glucocorticoid suppression of proasthmatic airway smooth muscle function.
    Authors: Hu A, Fatma S, Cao J, Grunstein JS, Nino G, Grumbach Y, Grunstein MM
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(5):L790-803.
    Species: Human
    Sample Type: Cell Lysates
  33. Glucocorticoid stress hormones and the effect of predation risk on elk reproduction.
    Authors: Creel S, Winnie JA, Christianson D
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(30):12388-93.
    Species: Elk
    Sample Type: Feces
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