hh Cortisol Parameter Assay Kit KGE008B: R&D Systems

Cortisol Parameter Assay Kit

(26 citations)   
  • Assay Length
    2.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (10 uL), Serum (10 uL), Saliva (20 uL), Urine (10 uL)
  • Sensitivity
    0.111 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Saliva, Urine)
  • Specificity
    Measures Cortisol levels in various samples
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC68, Parameter Immunoassay Control Set 901 for Cortisol - Please Inquire
Product Summary
The Parameter Cortisol Immunoassay is a 2.5 hour competitive enzyme immunoassay designed to measure Cortisol in cell culture supernates, serum, saliva, and urine.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Saliva, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean1.34.56.511.113.425.54
Standard Deviation0.120.2410.4110.2350.3190.576
CV%9.25.46.321.29.310.4

Recovery

The recovery of Cortisol spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 87-113
Saliva (n=4) 101 80-118
Serum (n=4) 104 92-114
Urine (n=4) 92 78-113
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Cortisol were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Cortisol Parameter Assay Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Cortisol
Cortisol, also known as hydrocortisone or compound F, is the primary glucocorticoid produced and secreted by the adrenal cortex. Found either free or bound to corticosteroid-binding globulin (CBG) in the blood, cortisol is involved in glucose homeostasis, inflammation, hypersensitivity, immunosuppression, and disease resistance. Plasma cortisol levels increase in response to stress and abnormal levels are implicated in prostate cancer, depression, and schizophrenia. Cushing's disease is caused by systemic cortisol excess.
    • Alternate Names
      Compound F; Cortisol; Hydrocortisone;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    26 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Chronic grouped social restriction triggers long-lasting immune system adaptations
      Authors: R Tian, G Hou, L Song, J Zhang, TF Yuan
      Oncotarget, 2017;8(20):33652-33657.
      Species: Human
      Sample Type: Serum
    2. Beneficial effect of combined treatment with octreotide and pasireotide in PCK rats, an orthologous model of human autosomal recessive polycystic kidney disease
      Authors: M Kugita, K Nishii, T Yamaguchi, A Suzuki, Y Yuzawa, S Horie, E Higashihar, S Nagao
      PLoS ONE, 2017;12(5):e0177934.
      Species: Rat
      Sample Type: Serum
    3. Flavour-enhanced cortisol release during gum chewing
      Authors: Y Hasegawa, Y Tachibana, T Ono, H Kishimoto
      PLoS ONE, 2017;12(4):e0173475.
      Species: Human
      Sample Type: Saliva
    4. Long-term Levodopa Treatment Accelerates the Circadian Rhythm Dysfunction in a 6-hydroxydopamine Rat Model of Parkinson's Disease
      Authors: SY Li, YL Wang, WW Liu, DJ Lyu, F Wang, CJ Mao, YP Yang, LF Hu, CF Liu
      Chin. Med. J., 2017;130(9):1085-1092.
      Species: Rat
      Sample Type: Plasma
    5. Intense Exercise Promotes Adult Hippocampal Neurogenesis But Not Spatial Discrimination
      Authors: JH So, C Huang, M Ge, G Cai, L Zhang, Y Lu, Y Mu
      Front Cell Neurosci, 2017;11(0):13.
      Species: Mouse
      Sample Type: Serum
    6. Novel 11?-hydroxysteroid dehydrogenase 1 inhibitors reduce cortisol levels in keratinocytes and improve dermal collagen content in human ex vivo skin after exposure to cortisone and UV
      Authors: SM Boudon, A Vuorinen, P Geotti-Bia, E Wandeler, DV Kratschmar, M Heidl, R Campiche, E Jackson, A Odermatt
      PLoS ONE, 2017;12(2):e0171079.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Relaxation - Induced by Vibroacoustic Stimulation via a Body Monochord and via Relaxation Music - Is Associated with a Decrease in Tonic Electrodermal Activity and an Increase of the Salivary Cortisol Level in Patients with Psychosomatic Disorders
      Authors: H Sandler, U Fendel, P Buáe, M Rose, R B”sel, BF Klapp
      PLoS ONE, 2017;12(1):e0170411.
      Species: Human
      Sample Type: Saliva
    8. Circadian variations in clinical symptoms and concentrations of inflammatory cytokines, melatonin, and cortisol in polymyalgia rheumatica before and during prednisolone treatment: a controlled, observational, clinical experimental study
      Arthritis Res Ther, 2016;18(1):174.
      Species: Human
      Sample Type: Plasma
    9. Effect of Peripheral Electrical Stimulation (PES) on Nocturnal Blood Glucose in Type 2 Diabetes: A Randomized Crossover Pilot Study
      PLoS ONE, 2016;11(12):e0168805.
      Species: Human
      Sample Type: Serum
    10. Anti-Inflammatory Modulation of Microglia via CD163-Targeted Glucocorticoids Protects Dopaminergic Neurons in the 6-OHDA Parkinson's Disease Model
      J Neurosci, 2016;36(36):9375-90.
    11. The Differential Hormonal Milieu of Morning versus Evening May Have an Impact on Muscle Hypertrophic Potential
      PLoS ONE, 2016;11(9):e0161500.
      Species: Human
      Sample Type: Serum
    12. Urinary cortisol and depression in early pregnancy: role of adiposity and race.
      Authors: Luiza J, Gallaher M, Powers R
      BMC Pregnancy Childbirth, 2015;15(0):30.
      Species: Human
      Sample Type: Plasma
    13. Metabolic implications when employing heavy pre- and post-exercise rapid-acting insulin reductions to prevent hypoglycaemia in type 1 diabetes patients: a randomised clinical trial.
      Authors: Campbell M, Walker M, Trenell M, Luzio S, Dunseath G, Tuner D, Bracken R, Bain S, Russell M, Stevenson E, West D
      PLoS ONE, 2014;9(5):e97143.
      Species: Human
      Sample Type: Serum
    14. Comparative transcriptomics of Atlantic Salmo salar, chum Oncorhynchus keta and pink salmon O. gorbuscha during infections with salmon lice Lepeophtheirus salmonis.
      Authors: Sutherland, Ben J G, Koczka, Kim W, Yasuike, Motoshig, Jantzen, Stuart G, Yazawa, Ryosuke, Koop, Ben F, Jones, Simon R
      BMC Genomics, 2014;15(0):200.
      Species: Fish - Salmon
      Sample Type: Plasma
    15. Hyperglycemia is associated with enhanced gluconeogenesis in a rat model of permanent cerebral ischemia.
      Authors: Wang Y, Chen C, Lin S, Chuang Y, Sheu W, Tung K
      Mol Cell Endocrinol, 2014;367(1):50-6.
      Species: Rat
      Sample Type: Plasma
    16. Placental P-glycoprotein is unaffected by timing of antenatal glucocorticoid therapy but reduced in SGA preterm infants.
      Authors: Hodyl N, Stark M, Butler M, Clifton V
      Placenta, 2013;34(4):325-30.
      Species: Human
      Sample Type: Serum
    17. Involvement of GR and p300 in the induction of H6PD by cortisol in human amnion fibroblasts.
      Authors: Wang W, Guo C, Li W, Li J, Wang W, Myatt L, Sun K
      Endocrinology, 2012;153(12):5993-6002.
      Species: Human
      Sample Type: Cell Culture Supernates
    18. Cortisol Synthesis in Epidermis Is Induced by IL-1 and Tissue Injury.
      Authors: Vukelic S, Stojadinovic O, Pastar I, Rabach M, Krzyzanowska A, Lebrun E, Davis SC, Resnik S, Brem H, Tomic-Canic M
      J. Biol. Chem., 2011;286(12):10265-75.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. Steroid synthesis by primary human keratinocytes; implications for skin disease.
      Authors: Hannen RF, Michael AE, Jaulim A, Bhogal R, Burrin JM, Philpott MP
      Biochem. Biophys. Res. Commun., 2011;404(1):62-7.
      Species: Human
      Sample Type: Cell Culture Supernates
    20. Is there a morning-to-evening difference in the acute IL-6 and cortisol responses to resistance exercise?
      Authors: Pledge D, Grosset JF, Onambele-Pearson GL
      Cytokine, 2011;55(2):318-23.
      Species: Human
      Sample Type: Serum
    21. Airway expression of the epithelial sodium channel alpha-subunit correlates with cortisol in term newborns.
      Authors: Janer C, Pitkanen OM, Helve O, Andersson S
      Pediatrics, 2011;128(2):e414-21.
      Species: Human
      Sample Type: Saliva
    22. Skeletal muscle myofibrillar and sarcoplasmic protein synthesis rates are affected differently by altitude-induced hypoxia in native lowlanders.
      Authors: Holm L, Haslund ML, Robach P, van Hall G, Calbet JA, Saltin B, Lundby C
      PLoS ONE, 2010;5(12):e15606.
      Species: Human
      Sample Type: Plasma
    23. Effects of exenatide on circulating glucose, insulin, glucagon, cortisol and catecholamines in healthy volunteers during exercise.
      Authors: Khoo EY, Wallis J, Tsintzas K, Macdonald IA, Mansell P
      Diabetologia, 2010;53(1):139-43.
      Species: Human
      Sample Type: Serum
    24. Immune perturbations in patients along the perioperative period: alterations in cell surface markers and leukocyte subtypes before and after surgery.
      Authors: Bartal I, Melamed R, Greenfeld K, Atzil S, Glasner A, Domankevich V, Naor R, Beilin B, Yardeni IZ, Ben-Eliyahu S
      Brain Behav. Immun., 2010;24(3):376-86.
      Species: Human
      Sample Type: Plasma
    25. Th2 cytokine-induced upregulation of 11beta-hydroxysteroid dehydrogenase-1 facilitates glucocorticoid suppression of proasthmatic airway smooth muscle function.
      Authors: Hu A, Fatma S, Cao J, Grunstein JS, Nino G, Grumbach Y, Grunstein MM
      Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(5):L790-803.
      Species: Human
      Sample Type: Cell Lysates
    26. Glucocorticoid stress hormones and the effect of predation risk on elk reproduction.
      Authors: Creel S, Winnie JA, Christianson D
      Proc. Natl. Acad. Sci. U.S.A., 2009;106(30):12388-93.
      Species: Elk
      Sample Type: Feces
    Expand to show all 26 Citations
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