Cortisol Parameter Assay Kit

(12 citations)   
  • Assay Length
    2.5 hours
  • Specificity
    Measures Cortisol levels in various samples
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Parameter Cortisol Immunoassay is a 2.5 hour competitive enzyme immunoassay designed to measure Cortisol in cell culture supernates, serum, saliva, and urine.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Saliva, Urine
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.120.2410.4110.2350.3190.576


The recovery of Cortisol spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 87-113
Saliva (n=4) 101 80-118
Serum (n=4) 104 92-114
Urine (n=4) 92 78-113
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Cortisol were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Cortisol Parameter Assay Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Cortisol
Cortisol, also known as hydrocortisone or compound F, is the primary glucocorticoid produced and secreted by the adrenal cortex. Found either free or bound to corticosteroid-binding globulin (CBG) in the blood, cortisol is involved in glucose homeostasis, inflammation, hypersensitivity, immunosuppression, and disease resistance. Plasma cortisol levels increase in response to stress and abnormal levels are implicated in prostate cancer, depression, and schizophrenia. Cushing's disease is caused by systemic cortisol excess.
    • Alternate Names
      Compound F; Hydrocortisone;
    Related Research Areas

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 12
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    Sample Type
    1. Metabolic implications when employing heavy pre- and post-exercise rapid-acting insulin reductions to prevent hypoglycaemia in type 1 diabetes patients: a randomised clinical trial.
      Authors: Campbell M, Walker M, Trenell M, Luzio S, Dunseath G, Tuner D, Bracken R, Bain S, Russell M, Stevenson E, West D
      PLoS ONE, 2014;9(5):e97143.
      Species: Human
      Sample Type: Serum
    2. Placental P-glycoprotein is unaffected by timing of antenatal glucocorticoid therapy but reduced in SGA preterm infants.
      Authors: Hodyl N, Stark M, Butler M, Clifton V
      Placenta, 2013;34(4):325-30.
      Species: Human
      Sample Type: Serum
    3. Involvement of GR and p300 in the induction of H6PD by cortisol in human amnion fibroblasts.
      Authors: Wang W, Guo C, Li W, Li J, Wang W, Myatt L, Sun K
      Endocrinology, 2012;153(12):5993-6002.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Is there a morning-to-evening difference in the acute IL-6 and cortisol responses to resistance exercise?
      Authors: Pledge D, Grosset JF, Onambele-Pearson GL
      Cytokine, 2011;55(2):318-23.
      Species: Human
      Sample Type: Serum
    5. Steroid synthesis by primary human keratinocytes; implications for skin disease.
      Authors: Hannen RF, Michael AE, Jaulim A, Bhogal R, Burrin JM, Philpott MP
      Biochem. Biophys. Res. Commun., 2011;404(1):62-7.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Airway expression of the epithelial sodium channel alpha-subunit correlates with cortisol in term newborns.
      Authors: Janer C, Pitkanen OM, Helve O, Andersson S
      Pediatrics, 2011;128(2):e414-21.
      Species: Human
      Sample Type: Saliva
    7. Cortisol Synthesis in Epidermis Is Induced by IL-1 and Tissue Injury.
      Authors: Vukelic S, Stojadinovic O, Pastar I, Rabach M, Krzyzanowska A, Lebrun E, Davis SC, Resnik S, Brem H, Tomic-Canic M
      J. Biol. Chem., 2011;286(12):10265-75.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. Effects of exenatide on circulating glucose, insulin, glucagon, cortisol and catecholamines in healthy volunteers during exercise.
      Authors: Khoo EY, Wallis J, Tsintzas K, Macdonald IA, Mansell P
      Diabetologia, 2010;53(1):139-43.
      Species: Human
      Sample Type: Serum
    9. Immune perturbations in patients along the perioperative period: alterations in cell surface markers and leukocyte subtypes before and after surgery.
      Authors: Bartal I, Melamed R, Greenfeld K, Atzil S, Glasner A, Domankevich V, Naor R, Beilin B, Yardeni IZ, Ben-Eliyahu S
      Brain Behav. Immun., 2010;24(3):376-86.
      Species: Human
      Sample Type: Plasma
    10. Skeletal muscle myofibrillar and sarcoplasmic protein synthesis rates are affected differently by altitude-induced hypoxia in native lowlanders.
      Authors: Holm L, Haslund ML, Robach P, van Hall G, Calbet JA, Saltin B, Lundby C
      PLoS ONE, 2010;5(12):e15606.
      Species: Human
      Sample Type: Plasma
    11. Th2 cytokine-induced upregulation of 11beta-hydroxysteroid dehydrogenase-1 facilitates glucocorticoid suppression of proasthmatic airway smooth muscle function.
      Authors: Hu A, Fatma S, Cao J, Grunstein JS, Nino G, Grumbach Y, Grunstein MM
      Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(5):L790-803.
      Species: Human
      Sample Type: Cell Lysates
    12. Glucocorticoid stress hormones and the effect of predation risk on elk reproduction.
      Authors: Creel S, Winnie JA, Christianson D
      Proc. Natl. Acad. Sci. U.S.A., 2009;106(30):12388-93.
      Species: Elk
      Sample Type: Feces
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    Parameter Immunoassay Control Set 901 for Cortisol

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