Cortisol Parameter Assay Kit

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Cortisol ELISA Standard Curve
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Product Details
Citations (43)
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Cortisol Parameter Assay Kit Summary

Assay Length
2.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (20 uL), Urine (10 uL)
0.111 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Measures Cortisol levels in various samples
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
No significant interference observed with available related molecules.

Product Summary

R&D Systems® Cortisol Immunoassay is a 2.5 hour competitive enzyme immunoassay designed to measure Cortisol in cell culture supernates, serum, plasma, saliva, and urine.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 1.3 4.5 6.51 1.11 3.42 5.54
Standard Deviation 0.12 0.241 0.411 0.235 0.319 0.576
CV% 9.2 5.4 6.3 21.2 9.3 10.4


The recovery of Cortisol spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 87-113
EDTA Plasma (n=4) 97 94-103
Heparin Plasma (n=4) 98 92-107
Saliva (n=4) 101 88-118
Serum (n=4) 95 89-100
Urine (n=4) 92 78-113


To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Cortisol were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Cortisol Linearity

Data Examples

Cortisol ELISA Standard Curve

Product Datasheets

Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Cortisol

Cortisol, also known as hydrocortisone or compound F, is the primary glucocorticoid produced and secreted by the adrenal cortex. Found either free or bound to corticosteroid-binding globulin (CBG) in the blood, cortisol is involved in glucose homeostasis, inflammation, hypersensitivity, immunosuppression, and disease resistance. Plasma cortisol levels increase in response to stress and abnormal levels are implicated in prostate cancer, depression, and schizophrenia. Cushing's disease is caused by systemic cortisol excess.

Alternate Names:
Compound F; Cortisol; Hydrocortisone
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Citations for Cortisol Parameter Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. Investigating Stress Response during Vaginal Delivery and Elective Cesarean Section through Assessment of Levels of Cortisol, Interleukin 6 (IL-6), Growth Hormone (GH) and Insulin-Like Growth Factor 1 (IGF-1)
    Authors: N Kiriakopou, S Grigoriadi, E Maziotis, A Philippou, A Rapani, P Giannelou, P Tsioulou, K Sfakianoud, A Kontogeorg, P Bakas, G Mastorakos, M Koutsilier, M Simopoulou
    J Clin Med, 2019;8(8):.
    Species: Human
    Sample Types: Serum
  2. The Influence of Psychological Stress, Depressive Symptoms, and Cortisol on Body Mass and Central Adiposity in 10- to-12-Year-Old Children
    Authors: T Lynch, A Azuero, JE Lochman, NJ Park, A Turner-Hen, M Rice
    J Pediatr Nurs, 2019;44(0):42-49.
    Species: Human
    Sample Types: Saliva
  3. Effect of stage of lactation and gestation on milking-induced hormone release in lactating dairy cows
    Authors: P Lacasse, X Zhao, S Ollier
    Domest. Anim. Endocrinol., 2019;66(0):72-85.
    Species: Bovine
    Sample Types: Serum
  4. Acute sleep loss results in tissue-specific alterations in genome-wide DNA methylation state and metabolic fuel utilization in humans
    Authors: J Cedernaes, M Schönke, JO Westholm, J Mi, A Chibalin, S Voisin, M Osler, H Vogel, K Hörnaeus, SL Dickson, SB Lind, J Bergquist, HB Schiöth, JR Zierath, C Benedict
    Sci Adv, 2018;4(8):eaar8590.
    Species: Human
    Sample Types: Serum
  5. Evaluation of Different Gases and Gas Combinations for On-Farm Euthanasia of Pre-Weaned Pigs
    Authors: N Kells, N Beausoleil, C Johnson, M Sutherland
    Animals (Basel), 2018;8(3):.
    Species: Porcine
    Sample Types: Plasma
  6. Effect of speed endurance training and reduced training volume on running economy and single muscle fiber adaptations in trained runners
    Authors: C Skovgaard, D Christians, PM Christense, NW Almquist, M Thomassen, J Bangsbo
    Physiol Rep, 2018;6(3):.
    Species: Human
    Sample Types: Plasma
  7. Psychological Stress Deteriorates Skin Barrier Function by Activating 11?-Hydroxysteroid Dehydrogenase 1 and the HPA Axis
    Authors: SJ Choe, D Kim, EJ Kim, JS Ahn, EJ Choi, ED Son, TR Lee, EH Choi
    Sci Rep, 2018;8(1):6334.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Relaxation - Induced by Vibroacoustic Stimulation via a Body Monochord and via Relaxation Music - Is Associated with a Decrease in Tonic Electrodermal Activity and an Increase of the Salivary Cortisol Level in Patients with Psychosomatic Disorders
    Authors: H Sandler, U Fendel, P Buáe, M Rose, R B”sel, BF Klapp
    PLoS ONE, 2017;12(1):e0170411.
    Species: Human
    Sample Types: Saliva
  9. Transcriptomic analyses and leukocyte telomere length measurement in subjects exposed to severe recent stressful life events
    Authors: N Lopizzo, S Tosato, V Begni, S Tomassi, N Cattane, M Barcella, G Turco, M Ruggeri, MA Riva, CM Pariante, A Cattaneo
    Transl Psychiatry, 2017;7(2):e1042.
    Species: Human
    Sample Types: Serum
  10. Ramadan model of intermittent fasting for 28�d had no major effect on body composition, glucose metabolism, or cognitive functions in healthy lean men
    Authors: NM Harder-Lau, A Rosenberg, FB Benatti, JA Damm, C Thomsen, EL Mortensen, BK Pedersen, R Krogh-Mads
    Nutrition, 2017;37(0):92-103.
    Species: Human
    Sample Types: Serum
  11. Flavour-enhanced cortisol release during gum chewing
    Authors: Y Hasegawa, Y Tachibana, T Ono, H Kishimoto
    PLoS ONE, 2017;12(4):e0173475.
    Species: Human
    Sample Types: Saliva
  12. Chronic grouped social restriction triggers long-lasting immune system adaptations
    Authors: R Tian, G Hou, L Song, J Zhang, TF Yuan
    Oncotarget, 2017;8(20):33652-33657.
    Species: Human
    Sample Types: Serum
  13. Novel 11?-hydroxysteroid dehydrogenase 1 inhibitors reduce cortisol levels in keratinocytes and improve dermal collagen content in human ex vivo skin after exposure to cortisone and UV
    Authors: SM Boudon, A Vuorinen, P Geotti-Bia, E Wandeler, DV Kratschmar, M Heidl, R Campiche, E Jackson, A Odermatt
    PLoS ONE, 2017;12(2):e0171079.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Intense Exercise Promotes Adult Hippocampal Neurogenesis But Not Spatial Discrimination
    Authors: JH So, C Huang, M Ge, G Cai, L Zhang, Y Lu, Y Mu
    Front Cell Neurosci, 2017;11(0):13.
    Species: Mouse
    Sample Types: Serum
  15. Topical mevastatin promotes wound healing by inhibiting the transcription factor c-Myc via the glucocorticoid receptor and the long noncoding RNA Gas5
    Authors: AP Sawaya, I Pastar, O Stojadinov, S Lazovic, SC Davis, J Gil, RS Kirsner, M Tomic-Cani
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  16. Long-term Levodopa Treatment Accelerates the Circadian Rhythm Dysfunction in a 6-hydroxydopamine Rat Model of Parkinson's Disease
    Authors: SY Li, YL Wang, WW Liu, DJ Lyu, F Wang, CJ Mao, YP Yang, LF Hu, CF Liu
    Chin. Med. J., 2017;130(9):1085-1092.
    Species: Rat
    Sample Types: Plasma
  17. Glucocorticoids modulate human brown adipose tissue thermogenesis in vivo
    Authors: H Scotney, ME Symonds, J Law, H Budge, D Sharkey, KN Manolopoul
    Metab. Clin. Exp., 2017;70(0):125-132.
    Species: Human
    Sample Types: Plasma
  18. Beneficial effect of combined treatment with octreotide and pasireotide in PCK rats, an orthologous model of human autosomal recessive polycystic kidney disease
    Authors: M Kugita, K Nishii, T Yamaguchi, A Suzuki, Y Yuzawa, S Horie, E Higashihar, S Nagao
    PLoS ONE, 2017;12(5):e0177934.
    Species: Rat
    Sample Types: Serum
  19. Effect of Peripheral Electrical Stimulation (PES) on Nocturnal Blood Glucose in Type 2 Diabetes: A Randomized Crossover Pilot Study
    PLoS ONE, 2016;11(12):e0168805.
    Species: Human
    Sample Types: Serum
  20. Anti-Inflammatory Modulation of Microglia via CD163-Targeted Glucocorticoids Protects Dopaminergic Neurons in the 6-OHDA Parkinson's Disease Model
    J Neurosci, 2016;36(36):9375-90.
  21. Effect of increased and maintained frequency of speed endurance training on performance and muscle adaptations in runners
    Authors: Jens Bangsbo
    J. Appl. Physiol., 2016;0(0):jap.00537.201.
    Species: Human
    Sample Types: Plasma
  22. Circadian variations in clinical symptoms and concentrations of inflammatory cytokines, melatonin, and cortisol in polymyalgia rheumatica before and during prednisolone treatment: a controlled, observational, clinical experimental study
    Arthritis Res Ther, 2016;18(1):174.
    Species: Human
    Sample Types: Plasma
  23. Tumor-Induced IL-6 Reprograms Host Metabolism to Suppress Anti-tumor Immunity
    Authors: Douglas T Fearon
    Cell Metab., 2016;24(5):672-684.
    Species: Human
    Sample Types: Serum
  24. The Differential Hormonal Milieu of Morning versus Evening May Have an Impact on Muscle Hypertrophic Potential
    PLoS ONE, 2016;11(9):e0161500.
    Species: Human
    Sample Types: Serum
  25. Urinary cortisol and depression in early pregnancy: role of adiposity and race.
    Authors: Luiza J, Gallaher M, Powers R
    BMC Pregnancy Childbirth, 2015;15(0):30.
    Species: Human
    Sample Types: Plasma
  26. Metabolic implications when employing heavy pre- and post-exercise rapid-acting insulin reductions to prevent hypoglycaemia in type 1 diabetes patients: a randomised clinical trial.
    Authors: Campbell M, Walker M, Trenell M, Luzio S, Dunseath G, Tuner D, Bracken R, Bain S, Russell M, Stevenson E, West D
    PLoS ONE, 2014;9(5):e97143.
    Species: Human
    Sample Types: Serum
  27. Hyperglycemia is associated with enhanced gluconeogenesis in a rat model of permanent cerebral ischemia.
    Authors: Wang Y, Chen C, Lin S, Chuang Y, Sheu W, Tung K
    Mol Cell Endocrinol, 2014;367(1):50-6.
    Species: Rat
    Sample Types: Plasma
  28. Comparative transcriptomics of Atlantic Salmo salar, chum Oncorhynchus keta and pink salmon O. gorbuscha during infections with salmon lice Lepeophtheirus salmonis.
    Authors: Sutherland, Ben J G, Koczka, Kim W, Yasuike, Motoshig, Jantzen, Stuart G, Yazawa, Ryosuke, Koop, Ben F, Jones, Simon R
    BMC Genomics, 2014;15(0):200.
    Species: Fish - Salmon
    Sample Types: Plasma
  29. Placental P-glycoprotein is unaffected by timing of antenatal glucocorticoid therapy but reduced in SGA preterm infants.
    Authors: Hodyl N, Stark M, Butler M, Clifton V
    Placenta, 2013;34(4):325-30.
    Species: Human
    Sample Types: Serum
  30. Involvement of GR and p300 in the induction of H6PD by cortisol in human amnion fibroblasts.
    Authors: Wang W, Guo C, Li W, Li J, Wang W, Myatt L, Sun K
    Endocrinology, 2012;153(12):5993-6002.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Airway expression of the epithelial sodium channel alpha-subunit correlates with cortisol in term newborns.
    Authors: Janer C, Pitkanen OM, Helve O, Andersson S
    Pediatrics, 2011;128(2):e414-21.
    Species: Human
    Sample Types: Saliva
  32. Steroid synthesis by primary human keratinocytes; implications for skin disease.
    Authors: Hannen RF, Michael AE, Jaulim A, Bhogal R, Burrin JM, Philpott MP
    Biochem. Biophys. Res. Commun., 2011;404(1):62-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Is there a morning-to-evening difference in the acute IL-6 and cortisol responses to resistance exercise?
    Authors: Pledge D, Grosset JF, Onambele-Pearson GL
    Cytokine, 2011;55(2):318-23.
    Species: Human
    Sample Types: Serum
  34. Cortisol Synthesis in Epidermis Is Induced by IL-1 and Tissue Injury.
    Authors: Vukelic S, Stojadinovic O, Pastar I, Rabach M, Krzyzanowska A, Lebrun E, Davis SC, Resnik S, Brem H, Tomic-Canic M
    J. Biol. Chem., 2011;286(12):10265-75.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Immune perturbations in patients along the perioperative period: alterations in cell surface markers and leukocyte subtypes before and after surgery.
    Authors: Bartal I, Melamed R, Greenfeld K, Atzil S, Glasner A, Domankevich V, Naor R, Beilin B, Yardeni IZ, Ben-Eliyahu S
    Brain Behav. Immun., 2010;24(3):376-86.
    Species: Human
    Sample Types: Plasma
  36. Effects of exenatide on circulating glucose, insulin, glucagon, cortisol and catecholamines in healthy volunteers during exercise.
    Authors: Khoo EY, Wallis J, Tsintzas K, Macdonald IA, Mansell P
    Diabetologia, 2010;53(1):139-43.
    Species: Human
    Sample Types: Serum
  37. Skeletal muscle myofibrillar and sarcoplasmic protein synthesis rates are affected differently by altitude-induced hypoxia in native lowlanders.
    Authors: Holm L, Haslund ML, Robach P, van Hall G, Calbet JA, Saltin B, Lundby C
    PLoS ONE, 2010;5(12):e15606.
    Species: Human
    Sample Types: Plasma
  38. Th2 cytokine-induced upregulation of 11beta-hydroxysteroid dehydrogenase-1 facilitates glucocorticoid suppression of proasthmatic airway smooth muscle function.
    Authors: Hu A, Fatma S, Cao J, Grunstein JS, Nino G, Grumbach Y, Grunstein MM
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(5):L790-803.
    Species: Human
    Sample Types: Cell Lysates
  39. Glucocorticoid stress hormones and the effect of predation risk on elk reproduction.
    Authors: Creel S, Winnie JA, Christianson D
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(30):12388-93.
    Species: Elk
    Sample Types: Feces
  40. Inhibition of 11beta-HSD2 expression by triclosan via induction of apoptosis in human placental syncytiotrophoblasts.
    Authors: Zhang N, Wang W, Li W, Liu C, Chen Y, Yang Q, Wang Y, Sun K
    J Clin Endocrinol Metab, 0;100(4):E542-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Induction of Amnion Epithelial Apoptosis by Cortisol via tPA/Plasmin System.
    Authors: Wang W, Liu C, Sun K
    Endocrinology, 0;157(11):4487-4498.
    Species: Human
    Sample Types: Tissue Homogenates
  42. Compartmentalized localization of 11beta-HSD 1 and 2 at the feto-maternal interface in the first trimester of human pregnancy.
    Authors: Yang Q, Wang W, Liu C, Wang Y, Sun K
    Placenta, 0;46(0):63-71.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. Effect of PFOS on glucocorticoid-induced changes in human decidual stromal cells in the first trimester of pregnancy.
    Authors: Yang Q, Wang W, Liu C, Wang Y, Sun K
    Reprod Toxicol, 0;63(0):142-50.
    Species: Human
    Sample Types: Cell Culture Supernates


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Cortisol Parameter Assay Kit
By Anonymous on 10/04/2019
Application: Sample Tested: Serum and Plasma

Cortisol Parameter Assay Kit
By Anonymous on 05/31/2018
Application: Sample Tested: tissue extract,Cell Culture Media