Cortisol, also known as hydrocortisone or compound F, is the primary glucocorticoid produced and secreted by the adrenal cortex. Found either free or bound to corticosteroid-binding globulin (CBG) in the blood, cortisol is involved in glucose homeostasis, inflammation, hypersensitivity, immunosuppression, and disease resistance. Plasma cortisol levels increase in response to stress and abnormal levels are implicated in prostate cancer, depression, and schizophrenia. Cushing's disease is caused by systemic cortisol excess.
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Cortisol Parameter Assay Kit
R&D Systems | Catalog # KGE008B
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Key Product Details
Assay Length
2.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (20 µL), Urine (10 µL)
Sensitivity
0.111 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
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Product Summary for Cortisol Parameter Assay Kit
R&D Systems® Cortisol Immunoassay is a 2.5 hour competitive enzyme immunoassay designed to measure Cortisol in cell culture supernates, serum, plasma, saliva, and urine.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
Biotin
Species
Multi-Species
Specificity
Measures Cortisol levels in various samples
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 1.30 | 4.50 | 6.51 | 1.11 | 3.42 | 5.54 |
| Standard Deviation | 0.120 | 0.241 | 0.411 | 0.235 | 0.319 | 0.576 |
| CV% | 9.2 | 5.4 | 6.3 | 21.2 | 9.3 | 10.4 |
Recovery for Cortisol Parameter Assay Kit
The recovery of Cortisol spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 87-113 |
| EDTA Plasma (n=4) | 97 | 94-103 |
| Heparin Plasma (n=4) | 98 | 92-107 |
| Saliva (n=4) | 101 | 88-118 |
| Serum (n=4) | 95 | 89-100 |
| Urine (n=4) | 92 | 78-113 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Cortisol were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Cortisol Parameter Assay Kit
Cortisol ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Cortisol
Alternate Names
Compound F, Hydrocortisone
Additional Cortisol Products
Product Documents for Cortisol Parameter Assay Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Cortisol Parameter Assay Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Cortisol Parameter Assay Kit
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3 Customer Ratings
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Sample Tested: Colon tissueVerified Customer | Posted 03/03/2023DRC
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