Cultrex Collagen I Cell Invasion Assay, 96-well

Asseses cell invasion through Collagen I
Catalog # Availability Size / Price Qty
3457-096-K
3457-024-K
Migration of NIH-3T3 and HT1080 Cells_Cultrex Cell Invasion_3457-096-K
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Cultrex Collagen I Cell Invasion Assay, 96-well Summary

The Cultrex Collagen I Cell Invasion Assay provides a tool for assessing cell invasion through Collagen I.

Why Use Cultrex Collagen I Cell Invasion Assay, 96-well?

Since different cell lines and different treatments can result in a wide range of invasive potentials, the permissiveness of each cell line to invade through Collagen I may be optimized to fit each experiment by adjusting the coating concentration. The Cultrex Collagen I Cell Invasion Assays are provided as either a single 96 well plate providing capacity for large screening experiments or as 24 individual inserts providing flexibility for smaller studies.

Specifications

Testing Cell Culture
Tested to support EMT-induced cell invasion and migration using A549 human lung carcinoma cell lines.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Bovine

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Data Example

FBS Stimulates Migration of NIH-3T3 and HT1080 Cells.  The NIH-3T3 mouse embryonic fibroblast cell line and the HT1080 human fibrosarcoma cell line were treated with 10% fetal bovine serum (FBS). The migration of untreated (yellow bars) and treated (green bars) NIH-3T3 and HT1080 cells against different extracellular matrix components, including Cultrex BME, Laminin I, Collagen I, Collagen IV, were quantified using the Cultrex Cell Invasion Assay Kits (Catalog # 3455-096-K3456-096-K3457-096-K3458-096-K, respectively). Data from four experiments was quantified for both non-invasive (NIH-3T3) and invasive (HT1080) cell types.

Reagents Provided

FAQs

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