Cultrex Collagen I Cell Invasion Assay, 96 well

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Citations (2)
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Cultrex Collagen I Cell Invasion Assay, 96 well Summary

Assay Principle

Cell Invasion Assay Principle
View Cell Invasion Assay Principle

The Cultrex® Collagen I Cell Invasion Assay is a 96-well microplate-based assay designed to accelerate the screening process for compounds that influence chemotaxis. Invasive migration is a fundamental function underlying cellular processes such as angiogenesis, embryonic development, immune response, metastasis, and invasion of cancer cells. The Cultrex Collagen I Cell Invasion Assay offers a flexible, standardized, high-throughput format for quantitating the degree to which invasive cells penetrate a barrier consisting of extracellular matrix protein Collagen I in response to chemoattractants and/or inhibiting compounds. This assay kit contains all the reagents needed for monitoring cell movement through Collagen I, including a simplified 96-well Boyden chamber with an 8 μm pore polyethylene terephthalate (PET) membrane, which is coated with the extracellular matrix by the end-user prior to use.

Kit Contents

  • Cell Invasion Chamber
  • Collagen I Coating Solution
  • Coating Buffer
  • Cell Wash Buffer
  • Cell Dissociation Solution
  • Calcein AM

Quantification of Cell Invasion through Different Extracellular Matrix Components

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FBS Stimulates Migration of NIH-3T3 and HT1080 Cells. The NIH-3T3 mouse embryonic fibroblast cell line and the HT1080 human fibrosarcoma cell line were treated with 10% fetal bovine serum (FBS). The migration of untreated (yellow bars) and treated (green bars) NIH-3T3 and HT1080 cells against different extracellular matrix components, including BME, Laminin I, Collagen I, Collagen IV, were quantified using the Cultrex Cell Invasion Assay Kits (Catalog # 3455-096-K, 3456-096-K, 3457-096-K, 3458-096-K, respectively). Data from four experiments was quantified for both non-invasive (NIH-3T3) and invasive (HT1080) cell types.

Cultrex is a registered trademark of Trevigen, Inc.

Product Datasheets

Preparation and Storage

Stability & Storage
The product requires storage at -20 to -70 °C, 2 - 8 °C, and room temperature.  Consult the product insert for specific storage temperatures. Do not use past expiration date.

Background: Collagen I

Collagens comprise a large family of insoluble extracellular glycoproteins that are essential components of connective tissues such as tendons, ligaments, cartilage, bone and skin. The mature polypeptides are secreted as coiled, left-handed helices that subsequently assemble into rope-like collagen fibers. Collagen I is a fibril-forming collagen that requires N- and C- terminal processing. Collagen IV is a network forming collagen whose C-terminus forms dimers and N-terminus forms tetramers.

Alternate Names
Collagen I

Citations for Cultrex Collagen I Cell Invasion Assay, 96 well

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A novel molecular pathway for Snail-dependent, SPARC-mediated invasion in non-small cell lung cancer pathogenesis.
    Authors: Grant J, Fishbein M, Hong L, Krysan K, Minna J, Shay J, Walser T, Dubinett S
    Cancer Prev Res (Phila), 0;7(1):150-60.  0
  2. Angiopoietin-like Protein 2 Is a Multistep Regulator of Inflammatory Neovascularization in a Murine Model of Age-related Macular Degeneration.
    Authors: Hirasawa M, Takubo K, Osada H, Miyake S, Toda E, Endo M, Umezawa K, Tsubota K, Oike Y, Ozawa Y
    J Biol Chem, 0;291(14):7373-85.  0


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