CXCL10/IP-10 is an ELR- angiostatic chemokine that interacts with CXCR3 to attract Th1 cells, eosinophils, monocytes, and NK cells to sites of inflammation. It is upregulated by activated T lymphocytes, neutrophils, splenocytes, keratinocytes, osteoblasts, astrocytes, endothelial cells, smooth muscle cells, and pancreatic beta cells. CXCL10 contributes to disease pathology in many chronic inflammatory disorders including rheumatoid arthritis, psoriasis, cancer, multiple sclerosis, diabetes, and cardiovascular disease.
CXCL10/IP-10/CRG-2 Antibody (JA10-82)
Novus Biologicals | Catalog # NBP2-67004
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]](https://resources.rndsystems.com/images/products/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunocytochemistry-Immunofluorescence-NBP2-67004-img0004.jpg "Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]")
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JA10-82 expressed in HEK293
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Product Specifications
Immunogen
Synthetic peptide within Human CXCL10/IP-10/CRG-2 aa 49-98 / 98. (SwissProt: P02778 Human)
Localization
Secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CXCL10/IP-10/CRG-2 Antibody (JA10-82)
Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]
Immunocytochemistry/Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Staining IP10 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]](https://resources.rndsystems.com/images/products/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunohistochemistry-Paraffin-NBP2-67004-img0005.jpg "Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]")
Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]
Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Human tonsil tissue using anti-IP10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.![Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]](https://resources.rndsystems.com/images/products/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunocytochemistry-Immunofluorescence-NBP2-67004-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]")
Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]
Immunocytochemistry/Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Staining IP10 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]](https://resources.rndsystems.com/images/products/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunocytochemistry-Immunofluorescence-NBP2-67004-img0003.jpg "Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]")
Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]
Immunocytochemistry/Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Staining IP10 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]](https://resources.rndsystems.com/images/products/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunohistochemistry-Paraffin-NBP2-67004-img0002.jpg "Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]")
Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]
Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Analysis of paraffin-embedded human skin tissue using anti-IP10 antibody. Counter stained with hematoxylin.Applications for CXCL10/IP-10/CRG-2 Antibody (JA10-82)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CXCL10/IP-10/CRG-2
Alternate Names
CRG-2, CRG2, IP-10
Gene Symbol
CXCL10
Additional CXCL10/IP-10/CRG-2 Products
Product Documents for CXCL10/IP-10/CRG-2 Antibody (JA10-82)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CXCL10/IP-10/CRG-2 Antibody (JA10-82)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for CXCL10/IP-10/CRG-2 Antibody (JA10-82)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
