CXCR4 Antibody - BSA Free

Novus Biologicals | Catalog # NLS1380

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken, Hamster, Monkey, Primate, Rabbit

Predicted:

Chimpanzee (100%), Opossum (100%), Orangutan (100%), Turkey (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CXCR4 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human CXCR4.

Epitope

3rd cytoplasmic domain

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Baboon (100%), Marmoset (100%).

Localization

Integral membrane protein.

Specificity

Human CXCR4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for CXCR4 Antibody - BSA Free

Immunohistochemistry-Paraffin: CXCR4 Antibody - BSA Free [NLS1380]

Immunohistochemistry-Paraffin: CXCR4 Antibody - BSA Free [NLS1380]

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Brain Cerebellum Purkinje Neuron
Immunohistochemistry-Paraffin: CXCR4 Antibody - BSA Free [NLS1380]

Immunohistochemistry-Paraffin: CXCR4 Antibody - BSA Free [NLS1380]

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Analysis of anti-CXCR4 antibody with human tonsil.
Immunohistochemistry-Paraffin: CXCR4 Antibody - BSA Free [NLS1380]

Immunohistochemistry-Paraffin: CXCR4 Antibody - BSA Free [NLS1380]

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Analysis of anti-CXCR4 antibody with human colon, carcinoma.
Immunohistochemistry-Paraffin: CXCR4 Antibody - BSA Free [NLS1380]

Immunohistochemistry-Paraffin: CXCR4 Antibody - BSA Free [NLS1380]

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Human Lymph Node, Non-Hodgkins Lymphoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Frozen: CXCR4 Antibody - BSA Free [NLS1380]

Immunohistochemistry-Frozen: CXCR4 Antibody - BSA Free [NLS1380]

Immunohistochemistry-Frozen: CXCR4 Antibody [NLS1380] - Rat brain tissue sections. Rat Nucleus Accumbens treated with MDPV. Images taken with fluorescent microscope. Primary antibody was diluted to 1:200. Secondary antibody used was donkey anti-Rabbit Alexa Fluor 594 at 1:500. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: CXCR4 Antibody - BSA Free [NLS1380]

Immunohistochemistry-Paraffin: CXCR4 Antibody - BSA Free [NLS1380]

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Lung, small cell carcinoma

Applications for CXCR4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

9-14 ug/mL
Application Notes
CXCR4 antibody validated for IHC-Fr from a verified customer review..

Reviewed Applications

Read 1 review rated 4 using NLS1380 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: CXCR4

CXCR4, a Chemokine Receptor involved in organ vascularization, neuronal cell migration, and patterning of the central nervous system during development. It binds stromal cell-derived factor 1 (SDF1, also called PBFS) and mediates migration of resting leukocytes and is unique in homing hematopoietic progenitors to bone marrow. These properties suggest that CXCR4 is involved in tumor cell migration and local tumor invasion. It is also an HIV-1 fusion co-factor that allows HIV-1 invasion in diverse human cell types. Although this receptor was initially called Neuropeptide Y3 Receptor, it does not respond to neuropeptide Y. CXCR4 has two isoforms that are produced by alternative splicing. CXCR4 has been reported to be expressed in cells of the immune and central nervous systems. It is highly expressed in brain and heart, and in white blood cells, vascular endothelial cells, and umbilical cord endothelial cells. In brain tumors, CXCR4 is expressed in regions of angiogenesis and degenerative, necrotic, and microcystic changes (Rempel et al., 2000). ESTs have been isolated from a wide variety of tissue libraries, particularly uterus, kidney, lung, tonsil, and brain.

Long Name

C-X-C Motif Chemokine Receptor 4

Alternate Names

CD184, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYRL, NPYY3R, WHIMS

Entrez Gene IDs

7852 (Human)

Gene Symbol

CXCR4

UniProt

P61073

Additional CXCR4 Products

Product Documents for CXCR4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CXCR4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CXCR4 Antibody - BSA Free

Customer Reviews for CXCR4 Antibody - BSA Free (1)

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Customer Images

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  • CXCR4 Antibody
    Name: Jeff Foss
    Application: Immunohistochemistry-Frozen
    Sample Tested: Brain Tissue Sections
    Species: Rat
    Verified Customer | Posted 08/20/2019
    Rat Nucleus Accumbens treated with MDPV. Images taken with fluorescent microscope. Primary was diluted to 1:200. Secondary used was Donkey Anti-Rabbit 594 (Life Technologies) 1:500.
    CXCR4 Antibody - BSA Free NLS1380

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Protocols

View specific protocols for CXCR4 Antibody - BSA Free (NLS1380):

Immunohistochemistry Protocol for CXCR4 Antibody (NLS1380):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CXCR4 Antibody - BSA Free

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  • Q: Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.

    A: CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

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