Cyclin A2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31330

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human, Mouse

Predicted:

Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cyclin A2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary.

Reactivity Notes

Pig (89%), Rabbit (83%), Bovine (84%).

Localization

Nucleus, Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cyclin A2 Antibody - BSA Free

Western Blot: Cyclin A2 Antibody [NBP1-31330]

Western Blot: Cyclin A2 Antibody [NBP1-31330]

Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0021.jpg
Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330]

Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330]

Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330] - 293T cells stained with Cyclin A2 antibody. Image from verified customer review.
Western Blot: Cyclin A2 Antibody [NBP1-31330]

Western Blot: Cyclin A2 Antibody [NBP1-31330]

Western Blot: Cyclin A2 Antibody [NBP1-31330] - 30 ug Neuro2A whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: Cyclin A2 Antibody [NBP1-31330]

Western Blot: Cyclin A2 Antibody [NBP1-31330]

Western Blot: Cyclin A2 Antibody [NBP1-31330] - 30 ug PC-12 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: Cyclin A2 Antibody [NBP1-31330]

Western Blot: Cyclin A2 Antibody [NBP1-31330]

Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at a dilution of 1:1000.
Western Blot: Cyclin A2 Antibody [NBP1-31330]

Western Blot: Cyclin A2 Antibody [NBP1-31330]

Western Blot: Cyclin A2 Antibody [NBP1-31330] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Cyclin A2 Antibody [NBP1-31330]

Western Blot: Cyclin A2 Antibody [NBP1-31330]

Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330]

Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330]

Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330] - NCIN87 Xenograft, using cyclin A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunoprecipitation: Cyclin A2 Antibody [NBP1-31330]

Immunoprecipitation: Cyclin A2 Antibody [NBP1-31330]

Immunoprecipitation: Cyclin A2 Antibody [NBP1-31330] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of Cyclin A2 protein by 2. 5 ug of Cyclin A2 antibody 10% SDS-PAGE gel.
Cyclin A2 Antibody

Western Blot: Cyclin A2 Antibody [NBP1-31330] -

Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young & old MRC-5 & HFF fibroblasts measured by RNA-seq, qRT-PCR, & Western Blots. (a) Four genes commonly downregulated & (b) 8 genes commonly upregulated in both cell lines. (a, b) The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Solid colored bars represent MRC-5 while shaded boxes represent HFF cells. The height of the bars corresponds to the logarithmic fold-change (FC) of expression between the first & the last PD investigated here (RNA-seq: log 2 RPKM FC; qRT-PCR: log 2− delta delta CT; protein: log 2 expression ratio). Error bars indicate standard deviation from the mean. Changes statistically different comparing young & old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: ∗p < 0.05, ∗∗p < 0.01, & ∗∗∗p < 0.001. (c) The blots show the protein expression levels in MRC-5 & HFF cells at young compared to old PDs. The up- or downregulation was signified by the presence or absence of bands in Western Blots. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26339636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Cyclin A2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Cyclin A2 Antibody [NBP1-31330] -

Cyclin A2 antibody detects Cyclin A2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin A2 stained by Cyclin A2 antibody (NBP1-31330) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 um.

Applications for Cyclin A2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Application Notes
Cyclin A2 antibody validated for IHC-P, WB from verified customer reviews.

Reviewed Applications

Read 3 reviews rated 4.7 using NBP1-31330 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cyclin A2

Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin / cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S phase and passage through G2 phase.

Alternate Names

CCNA, CCNA2

Gene Symbol

CCNA2

Additional Cyclin A2 Products

Product Documents for Cyclin A2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cyclin A2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Cyclin A2 Antibody - BSA Free

Customer Reviews for Cyclin A2 Antibody - BSA Free (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell
    Species: Human
    Verified Customer | Posted 10/04/2015
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: 293T cell line
    Species: Human
    Verified Customer | Posted 10/02/2015
    Cyclin A2 on 293T
    Cyclin A2 Antibody - BSA Free NBP1-31330
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Whole cell lysate from HeLa cells
    Species: Human
    Verified Customer | Posted 09/19/2014
    Cyclin A2 WB in Hela cells
    Cyclin A2 Antibody - BSA Free NBP1-31330

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Protocols

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