Cytokeratin 19 Antibody (SB39g) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-28570

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # SB39g

Format

Azide and BSA Free
View all Cytokeratin 19 Primary Antibodies »

Product Specifications

Immunogen

Recombinant C-terminal keratin 19

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Epithelial Cell Marker

Specificity

Keratin 19

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Cytokeratin 19 Antibody (SB39g) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (SB39g) [NBP1-28570]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (SB39g) [NBP1-28570]

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (SB39g) [NBP1-28570] - Cell cytospin of the HCC38 pancreatic carcinoma cell line was incubated with purified SB39g anti-keratin monoclonal antibody. After washing, a 10ug/mL dilution of goat anti-mouse Ig-FITC was used for immunofluorescence staining. Positive cells were viewed by fluorescence microscopy.
Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (SB39g) [NBP1-28570]

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (SB39g) [NBP1-28570]

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (SB39g) [NBP1-28570] - Human gastric cancer tissue was stained with Mouse IgG2a-HRP isotype control ( left) and Mouse Anti-Cytokeratin 19-HRP (right) followed by DAB and hematoxylin.

Applications for Cytokeratin 19 Antibody (SB39g) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:2000-1:4000

Reviewed Applications

Read 1 review rated 4 using NBP1-28570 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.1M BBS (pH 8.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cytokeratin 19

Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30% sequence homology. (2-6) Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 19 is the smallest human keratin, is found in many simple and some non-keratinizing stratified squamous epithelia, and is often co-expressed with keratin 7. (7,8) Cytokeratin 19 has been implicated as a marker for stem cells in some tissues, such as hair follicles in the skin. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. The degree of cytokeratin 19 positivity in breast cancer distinguishes malignant from benign tumors. Cytokeratin 19 is also associated with malignant transformation in esophageal and pancreatic cancers. (9,10)

Alternate Names

CK19, EndoC, K19, Krt19

Gene Symbol

KRT19

Additional Cytokeratin 19 Products

Product Documents for Cytokeratin 19 Antibody (SB39g) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cytokeratin 19 Antibody (SB39g) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Cytokeratin 19 Antibody (SB39g) - Azide and BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: mouse embryonic fibroblast cell line, Sample Amount: 40 ug
    Species: Mouse
    Verified Customer | Posted 01/21/2010

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Protocols

View specific protocols for Cytokeratin 19 Antibody (SB39g) - Azide and BSA Free (NBP1-28570):

NBP1-28570 Western blot Protocol (NBP1-28570):
Run protein on a 14% gel, using HCC38 & HT-29 cell lysate at 20 ug/well. (No sonication). Dilute NBP1-28570 to 2 ug/mL in 5% milk/TBST. 6mL/strip, incubated at RT on shaker for 1 hour. Dilute anti-mouse secondary antibody at 1:4K in 5% milk/TBST, 6mL/strip, incubate at RT on shaker for 1 hour.

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FAQs

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