DDX1 Antibody (3E5E2) - BSA Free

Novus Biologicals | Catalog # NBP2-61745

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3E5E2

Format

BSA Free
View all DDX1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human DDX1 (AA: 642-740) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

82.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DDX1 Antibody (3E5E2) - BSA Free

DDX1 Antibody (3E5E2) - BSA Free Western Blot Shows DDX1 Specificity Using Knockout Cell Line.

Western Blot Shows DDX1 Specificity Using Knockout Cell Line.

Western blot shows lysates of U2OS human osteosarcoma parental cell line and DDX1 knockout U2OS cell line (KO). Nitrocellulose membrane was probed with DDX1 Antibody (3E5E2) - BSA Free (Catalog # NBP2-61745) followed by HRP-conjugated secondary antibody. A specific band was detected for DDX1 at approximately 82.4 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. Primary antibody dilution used: 1/500. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
DDX1 Antibody (3E5E2) - BSA Free Detection of DDX1 by Immunoprecipitation.

Detection of DDX1 by Immunoprecipitation.

U2OS human osteosarcoma cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of DDX1 Antibody (3E5E2) - BSA Free (Catalog # NBP2-61745) pre-coupled to Dynabeads Protein G. Immunoprecipitated DDX1 was detected in Western Blot with a rabbit DDX1 antibody used at 1/1000. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Western Blot: DDX1 Antibody (3E5E2)BSA Free [NBP2-61745]

Western Blot: DDX1 Antibody (3E5E2)BSA Free [NBP2-61745]

Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mAb against HEK293 (1) and DDX1 (AA: 642-740)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: DDX1 Antibody (3E5E2) - BSA Free [NBP2-61745]

Immunocytochemistry/ Immunofluorescence: DDX1 Antibody (3E5E2) - BSA Free [NBP2-61745]

Immunocytochemistry/Immunofluorescence: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of Hela cells using DDX1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) - BSA Free [NBP2-61745]

Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) - BSA Free [NBP2-61745]

Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of bladder cancer tissues using DDX1 mouse mAb with DAB staining.
Flow Cytometry: DDX1 Antibody (3E5E2) - BSA Free [NBP2-61745]

Flow Cytometry: DDX1 Antibody (3E5E2) - BSA Free [NBP2-61745]

Flow Cytometry: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of Hela cells using DDX1 mouse mAb (green) and negative control (red).
Western Blot: DDX1 Antibody (3E5E2)BSA Free [NBP2-61745]

Western Blot: DDX1 Antibody (3E5E2)BSA Free [NBP2-61745]

Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mAb against human DDX1 (AA: 642-740) recombinant protein. (Expected MW is 37 kDa)
Western Blot: DDX1 Antibody (3E5E2)BSA Free [NBP2-61745]

Western Blot: DDX1 Antibody (3E5E2)BSA Free [NBP2-61745]

Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mouse mAb against Hela (1), MCF-7 (2), A431 (3), PC-3 (4), NIH/3T3 (5), Jurkat (6), U251 (7), and HEK293 (8) cell lysate.
Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) - BSA Free [NBP2-61745]

Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) - BSA Free [NBP2-61745]

Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of colon cancer tissues using DDX1 mouse mAb with DAB staining.
ELISA: DDX1 Antibody (3E5E2) - BSA Free [NBP2-61745]

ELISA: DDX1 Antibody (3E5E2) - BSA Free [NBP2-61745]

ELISA: DDX1 Antibody (3E5E2) [NBP2-61745] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for DDX1 Antibody (3E5E2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Knockout Validated

Validated for Knockout from YCharOS Inc. (ycharos.com)

Western Blot

1:500-1:2000
Application Notes
Use in Immunoprecipitation reported in scientific literature (PMID:32755435)

Reviewed Applications

Read 1 review rated 5 using NBP2-61745 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX1

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.;

Long Name

DEAD-Box Helicase 1

Alternate Names

DBP-RB, DEAD Box-1, DEAD/H-Box Helicase 1, UKVH5d

Gene Symbol

DDX1

Additional DDX1 Products

Product Documents for DDX1 Antibody (3E5E2) - BSA Free

Certificate of Analysis

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Product Specific Notices for DDX1 Antibody (3E5E2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DDX1 Antibody (3E5E2) - BSA Free

Customer Reviews for DDX1 Antibody (3E5E2) - BSA Free (1)

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  • DDX1 Antibody (3E5E2)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: AC16
    Species: Human
    Verified Customer | Posted 05/07/2020
    DDX1
    DDX1 Antibody (3E5E2) - BSA Free NBP2-61745

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