DEPTOR/DEPDC6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85256

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DEPTOR/DEPDC6 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LVTGEQLRLRLHEEKVIKDRRHHLKTYPNCFVAKELIDWLIEHKEASDRETAIKLMQKLADRGIIHHVCDEH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DEPTOR/DEPDC6 Antibody - BSA Free

Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-85256]

Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-85256]

Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-85256] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: DEPTOR/DEPDC6 Antibody [NBP1-85256]

Immunohistochemistry-Paraffin: DEPTOR/DEPDC6 Antibody [NBP1-85256]

Immunohistochemistry-Paraffin: DEPTOR/DEPDC6 Antibody [NBP1-85256] - Staining of human pancreas shows low expression as expected.
Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-85256]

Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-85256]

Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-85256] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: Human cell line RT-4
Immunohistochemistry: DEPTOR/DEPDC6 Antibody [NBP1-85256]

Immunohistochemistry: DEPTOR/DEPDC6 Antibody [NBP1-85256]

Immunohistochemistry: DEPTOR/DEPDC6 Antibody [NBP1-85256] - Staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.
Immunohistochemistry: DEPTOR/DEPDC6 Antibody [NBP1-85256]

Immunohistochemistry: DEPTOR/DEPDC6 Antibody [NBP1-85256]

Immunohistochemistry: DEPTOR/DEPDC6 Antibody [NBP1-85256] - Staining of human adrenal gland shows high expression.
Immunohistochemistry-Paraffin: DEPTOR/DEPDC6 Antibody [NBP1-85256]

Immunohistochemistry-Paraffin: DEPTOR/DEPDC6 Antibody [NBP1-85256]

Immunohistochemistry-Paraffin: DEPTOR/DEPDC6 Antibody [NBP1-85256] - Staining in human adrenal gland and pancreas tissues using anti-DEPTOR antibody. Corresponding DEPTOR RNA-seq data are presented for the same tissues.

Applications for DEPTOR/DEPDC6 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DEPTOR/DEPDC6

DEPTOR is a negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of bothcomplexes

Long Name

DEP Domain Containing mTOR-interacting Protein

Alternate Names

DEP.6, DEPDC6

Gene Symbol

DEPTOR

Additional DEPTOR/DEPDC6 Products

Product Documents for DEPTOR/DEPDC6 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DEPTOR/DEPDC6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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