Desmin Antibody - C-terminus

Novus Biologicals | Catalog # NBP1-45143

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Desmin Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide sequence C-RDGEVVSEATQQQHE from the C-Terminal region of Desmin (NP_001918.3)

Reactivity Notes

Predicted cross-reactivities: Mouse, Porcine, Rat, Dog, Bovine

Specificity

Goat anti Human Desmin recognizes an epitope within the C-terminal (CT) region of human.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Desmin Antibody - C-terminus

Western Blot: Desmin Antibody [NBP1-45143]

Western Blot: Desmin Antibody [NBP1-45143]

Western Blot: Desmin Antibody [NBP1-45143] - Analysis of human (A), mouse (B), rat (C) and pig (D) skeletal muscle lysate (35ug protein in RIPA buffer) probed with Goat anti Human desmin antibody (NBP1-45143) at 1.0ugml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence
Immunohistochemistry-Paraffin: Desmin Antibody [NBP1-45143]

Immunohistochemistry-Paraffin: Desmin Antibody [NBP1-45143]

Immunohistochemistry-Paraffin: Desmin Antibody [NBP1-45143] - Staining showing strong specific labeling in pericytes around blood vessels in the brain. Antigen retrieval at pH6 (citra) was performed and Desmin was used at 1:500 dilution. Hematoxyllin was used as a counterstain to mark nuclei. Image from verified customer review.
Western Blot: Desmin Antibody [NBP1-45143]

Western Blot: Desmin Antibody [NBP1-45143]

Western Blot: Desmin Antibody [NBP1-45143] - Human skeletal muscle cell lysate probed with Goat anti Human Desmin C-Terminal (NBP1-45143)

Applications for Desmin Antibody - C-terminus

Application
Recommended Usage

ELISA

1:2000

Immunohistochemistry

3-6 ug/ml

Immunohistochemistry-Paraffin

3-6 ug/ml

Western Blot

0.1-0.3 ug/ml
Application Notes
This product requires heat-mediated antigen retrieval prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. Goat anti Human Desmin detects a band of approximately 55kDa in human skeletal muscle cell lysates.

Reviewed Applications

Read 1 review rated 5 using NBP1-45143 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TRIS buffered saline, 0.5% Bovine Serum Albumin

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Desmin

Desmins belongs to the intermediate filament family, and are class III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z line structures. Defects in Desmin are the cause of desmin related cardio skeletal myopathy (CSM) also known as desmin related myopathy (DRM). CSM is characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by intracytoplasmic accumulation of desmin reactive deposits in cardiac and skeletal muscle cells. A desmin related myopathy can have a distal onset, it is then known as hereditary distal myopathy (HDM). Defects in Desmin are also the cause of dilated cardiomyopathy type 1I (CMD1I). CMD1I is an autosomal form of dilated cardiomyopathy characterized by ventricular dilatation and impaired systolic function. Antidesmin antibodies are useful in identification of tumours of myogenic origin.

Alternate Names

CMD1I, DES

Entrez Gene IDs

1674 (Human)

Gene Symbol

DES

UniProt

P17661

Additional Desmin Products

Product Documents for Desmin Antibody - C-terminus

Certificate of Analysis

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Product Specific Notices for Desmin Antibody - C-terminus

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cardiac Stem Cell Markers

Citations for Desmin Antibody - C-terminus

Customer Reviews for Desmin Antibody - C-terminus (1)

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  • Desmin Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: brain and spinal cord
    Species: Human
    Verified Customer | Posted 03/01/2019
    Desmin staining showing strong specific labeling in pericytes around blood vessels in the brain. Antigen retrieval at pH6 (citra) was performed and Desmin was used at 1:500 dilution. Hematoxyllin was used as a counterstain to mark nuclei
    Antigen retrieval at pH6 fro 20 minutes in a steamer; Used at 1:500 concentration. Novared (Vector labs) was used as a chromogen to develop the signal.
    Desmin Antibody - C-terminus NBP1-45143

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