DGAT1 Antibody

Flow Cytometry: DGAT1 Antibody [NB100-57086]

Flow Cytometry: DGAT1 Antibody [NB100-57086] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western Blot: DGAT1 Antibody [NB100-57086] -

Lipidomic analysis.Intestinal LD contains TAG but also DAG, MAG and FFA and lipid metabolizing enzymes A) TLC analysis of neutrals lipids from initial homogenate (I), the LD D1, D2 and D3, the fraction M containing microsomes and membrane and the last infranatant (S). Lipids were stained using 25% H2SO4 and heated what led to stain in blue free (FC) and esterified (EC) cholesterols and in brown free fatty acids (FFA), monoacylglycerol (MAG), diacylglycerol (DAG, either 1,2 DAG or 1,3 DAG) and triacylglycerol (TAG). Sample volumes were adapted to permit concomitant lipid visualization in a unique thin layer. S was almost exempt of lipids even after concentration. B) Acyl esterase assay were performed with equivalent samples of D1, D2 and D3. Lipids were extracted, separated by TLC, stained with iodine vapor. The bands corresponding to FFA, MAG, DAG and TAG were scrapped and the incorporated radio-activity was quantified by liquid scintillation counting. Data are expressed in means of 103 dpm/equivalent sample +/- SEM, n = 3 separate experiments. C) Immunodetection of ADFP (adipocyte differenciation-related protein), MTTP (microsomal triglyceride transfer protein), ACAT1 and ACAT2 (acetyl-CoA cholesterol acyl transferase), MGAT2 (monoacyl glycerol acyl transferase 2), HSL (Hormone sensitive lipase) and ATGL (adipose triglyceride lipase). ACAT1 and ACAT2 were selectively detected in distinct bands that are clearly distinguished after co-incubation with anti-ACAT1 and anti-ACAT2 antibodies. For each sample, equivalent amounts of proteins (60ug) were subjected to SDS-PAGE and transferred on nitrocellulose membrane. All data in the figure are representative of 3 separate experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23560035), licensed under a CC-BY license. Not internally tested by Novus Biologicals.