DNAM-1/CD226 Antibody (BLR063G) - BSA Free

Novus Biologicals | Catalog # NBP2-77357

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR063G

Format

BSA Free
View all DNAM-1/CD226 Primary Antibodies »

Product Specifications

Immunogen

Immunogen was a recombinant protein representing the extracellular domain of human DNAM1/CD226.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNAM-1/CD226 Antibody (BLR063G) - BSA Free

Western Blot: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357]

Western Blot: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357]

Western Blot: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357] - Whole cell lysate (10 ug) from SUP-M2, RT-4, HH, U-251MG, MJ, HDLM-2, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-DNAM-1/CD226 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
Immunocytochemistry/ Immunofluorescence: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357]

Immunocytochemistry/ Immunofluorescence: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357]

Immunocytochemistry/Immunofluorescence: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357] - Rabbit anti-DNAM-1 recombinant monoclonal antibody [BLR063G]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry-Paraffin: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357]

Immunohistochemistry-Paraffin: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357]

Immunohistochemistry-Paraffin: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357] - Rabbit anti-DNAM-1 recombinant monoclonal antibody [BLR063G]. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Immunocytochemistry/ Immunofluorescence: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357]

Immunocytochemistry/ Immunofluorescence: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357]

Immunocytochemistry/Immunofluorescence: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357] - Rabbit anti-DNAM-1 recombinant monoclonal antibody [BLR063G]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate:DAB.
Immunoprecipitation: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357]

Immunoprecipitation: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357]

Immunoprecipitation: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357] - Detection of human DNAM-1/CD226 by WB of immunoprecipitates from HH lysate. Antibodies: Rabbit anti-DNAM-1/CD226 recombinant monoclonal [BLR063G] (NBP2-77357) and another rabbit anti-DNAM-1/CD226 recombinant monoclonal [BL-799B-1B9].

Applications for DNAM-1/CD226 Antibody (BLR063G) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

20 ul/mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Borate Buffered Saline (BBS) pH 8.2

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: DNAM-1/CD226

CD226 is a 65kD type I transmembrane glycoprotein, known as DNAM-1 (DNAX accessory molecule-1), PTA1(platelet and T cell activation antigen 1), or TLiSA1 (T lineage-specific activation antigen 1 antigen). It belongs to immunoglobulin superfamily containing 2 Ig-like domains, is expressed on majority of T lymphocytes, NK cells, monocytes, platelets, and a subset of B cells, activated endothelial cells. DNAM-1 is also expressed on CD8+ (but not CD8-) dendritic cells in mouse model. CD226 is an adhesion molecule involved in CTL and NK cell-mediated cytotoxicity. It has been identified that CD155 (poliovirus receptor, PVR) and CD112 (nectin-2, PRR-2) are CD226 ligands. DX11 antibody is able to inhibit CTL and NK cell-mediated cytotoxicity, and to block T cell cytokine production.

Long Name

DNAX Accessory Molecule 1

Alternate Names

CD226, DNAM1, PTA1, TLiSA1

Gene Symbol

CD226

Additional DNAM-1/CD226 Products

Product Documents for DNAM-1/CD226 Antibody (BLR063G) - BSA Free

Certificate of Analysis

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Product Specific Notices for DNAM-1/CD226 Antibody (BLR063G) - BSA Free

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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