E-Cadherin Antibody (ST54-01)

Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540]

Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 130 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (ST54-01) [NBP2-67540]

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Staining Pan-Cadherin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Pan-Cadherin antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibodfor 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.