Key Product Details

Species Reactivity

Human, Primate

Applications

Immunohistochemistry, ELISA Standard (Matched Pair), ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair)

Label

Janelia Fluor 549

Antibody Source

Monoclonal Mouse IgG1 Clone # 10827
View all EGF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human EGF

Specificity

Detects human and primate EGF in ELISAs. In sandwich ELISAs, no cross-reactivity or interference is observed with recombinant mouse (rm) EGF, bovine FGF acidic, bovine FGF basic, recombinant human (rh) G-CSF, rhGM-CSF, rmGM-CSF, rhLIF, human PDGF, porcine PDGF, rhTGF‑ alpha, hTGF-beta 1, pTGF-beta 1, rhTGF-beta 1, porcine TGF-beta 1.2, porcine TGF-beta 2, rhTNF-alpha, or rhTNF-beta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for EGF Antibody (10827) [Janelia Fluor® 549]

Application
Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: EGF

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.

Long Name

Epidermal Growth Factor

Alternate Names

HOMG4, URG, Urogastrone

Gene Symbol

EGF

Additional EGF Products

Product Documents for EGF Antibody (10827) [Janelia Fluor® 549]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for EGF Antibody (10827) [Janelia Fluor® 549]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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