Detects human and primate EGF in ELISAs. In sandwich ELISAs, no cross-reactivity or interference is observed with recombinant mouse (rm) EGF, bovine FGF acidic, bovine FGF basic, recombinant human (rh) G-CSF, rhGM-CSF, rmGM-CSF, rhLIF, human PDGF, porcine PDGF, rhTGF‑ alpha, hTGF-beta 1, pTGF-beta 1, rhTGF-beta 1, porcine TGF-beta 1.2, porcine TGF-beta 2, rhTNF-alpha, or rhTNF-beta.
Monoclonal Mouse IgG1 Clone # 10827
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human EGF
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
EGF in Human Lung Cancer Tissue.
EGF was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human/Primate EGF Monoclonal Antibody (Catalog # MAB636) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
EGF is the prototypic member of a family of growth factors that are
characterized by the presence of EGF like domains and activate members
of the EGF receptor family. Proteolytic cleavage of a membrane-bound
precursor releases mature soluble EGF which interacts with the EGF R to
promote proliferation and differentiation of mesenchymal and epithelial
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but also provides information about sample types, species, and experimental conditions.
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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ELISA.