EGF Antibody (10827)

R&D Systems | Catalog # MAB636

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human, Bovine

Applications

Validated:

Immunohistochemistry, ELISA Capture (Matched Antibody Pair)

Cited:

Western Blot, ELISA Capture, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 10827
View all EGF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human EGF

Specificity

Detects human and primate EGF in ELISAs. In sandwich ELISAs, no cross-reactivity or interference is observed with recombinant mouse (rm) EGF, bovine FGF acidic, bovine FGF basic, recombinant human (rh) G-CSF, rhGM-CSF, rmGM-CSF, rhLIF, human PDGF, porcine PDGF, rhTGF‑ alpha, hTGF-beta 1, pTGF-beta 1, rhTGF-beta 1, porcine TGF-beta 1.2, porcine TGF-beta 2, rhTNF-alpha, or rhTNF-beta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for EGF Antibody (10827)

EGF antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P).

EGF in Human Lung Cancer Tissue.

EGF was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human/Primate EGF Monoclonal Antibody (Catalog # MAB636) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Human EGF ELISA Standard Curve

Human EGF ELISA Standard Curve

Recombinant Human EGF (Catalog # 236-EG) was serially diluted and captured by Mouse Anti-Human/Primate EGF Monoclonal Antibody (Catalog # MAB636) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for EGF Antibody (10827)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung cancer tissue

Human/Primate EGF Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human/Primate EGF Biotinylated Antibody (Catalog # BAF236)
  • Standard: Recombinant Human EGF Protein, CF (Catalog # 236-EG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB636 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EGF

EGF is the prototypic member of a family of growth factors that are characterized by the presence of EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.

Long Name

Epidermal Growth Factor

Alternate Names

HOMG4, URG, Urogastrone

Entrez Gene IDs

1950 (Human); 13645 (Mouse); 25313 (Rat)

Gene Symbol

EGF

Additional EGF Products

Product Documents for EGF Antibody (10827)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for EGF Antibody (10827)

For research use only

Citations for EGF Antibody (10827)

Customer Reviews for EGF Antibody (10827) (2)

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2 Customer Ratings
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Customer Images

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  • Human/Primate EGF Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 07/19/2019
    EGF Antibody (10827) MAB636
  • Human/Primate EGF Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/07/2017
    This antibody was used to develop a sandwich ELISA for the measurement of EGF in human serum. It worked very well using AF236 as a matched pair.
    EGF Antibody (10827) MAB636

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Protocols

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