Human/Primate EGF Antibody

(2 citations)
(1 Review)
  
  • Species Reactivity
    Human, Primate
  • Specificity
    Detects human and primate EGF in ELISAs. In sandwich ELISAs, no cross-reactivity or interference is observed with recombinant mouse (rm) EGF, bovine FGF acidic, bovine FGF basic, recombinant human (rh) G-CSF, rhGM-CSF, rmGM-CSF, rhLIF, human PDGF, porcine PDGF, rhTGF‑ alpha, hTGF-beta 1, pTGF-beta 1, rhTGF-beta 1, porcine TGF-beta 1.2, porcine TGF-beta 2, rhTNF-alpha, or rhTNF-beta.
  • Source
    Monoclonal Mouse IgG1 Clone # 10827
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human EGF
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
    • Human/Primate EGF Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human/Primate EGF Antibody (Catalog # MAB636)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human/Primate EGF Biotinylated Antibody (Catalog # BAF236)
  • ELISA Standard
     
    Recombinant Human EGF Protein, CF (Catalog # 236-EG)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
EGF in Human Lung Cancer Tissue. EGF was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human/Primate EGF Monoclonal Antibody (Catalog # MAB636) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EGF
EGF is the prototypic member of a family of growth factors that are characterized by the presence of EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.
  • Long Name:
    Epidermal Growth Factor
  • Entrez Gene IDs:
    1950 (Human); 13645 (Mouse); 25313 (Rat)
  • Alternate Names:
    beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012;378(1):72-80.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  2. Accumulation of hEGF and hEGF-fusion proteins in chloroplast-transformed tobacco plants is higher in the dark than in the light.
    Authors: Wirth S, Segretin ME, Mentaberry A, Bravo-Almonacid F
    J. Biotechnol., 2006;125(2):159-72.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

CTS002 14
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

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Antigen Retrieval Reagent-Basic

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Mounting Medium

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Antigen Retrieval Reagent Sampler (50 mL each)

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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Average Rating: 5 (Based on 1 review)

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ELISA Human/Primate EGF Antibody MAB636
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 ELISA Human Anonymous 11/07/2017
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ELISA Human/Primate EGF Antibody MAB636
ELISA: Human/Primate EGF Antibody [MAB636]

Summary

ApplicationELISA
Sample TestedSerum and Plasma
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThis antibody was used to develop a sandwich ELISA for the measurement of EGF in human serum. It worked very well using AF236 as a matched pair.

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