eIF4E Antibody (5D11)

Novus Biologicals | Catalog # NBP2-22177

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5D11
View all eIF4E Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human eIF4E expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF4E Antibody (5D11)

Western Blot: eIF4E Antibody (5D11)BSA Free [NBP2-22177]

Western Blot: eIF4E Antibody (5D11)BSA Free [NBP2-22177]

Western Blot: eIF4E Antibody (5D11) [NBP2-22177] - Western blot analysis using eIF4E mouse mAb against Hela (1), HEK293 (2) and K562 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: eIF4E Antibody (5D11) - BSA Free [NBP2-22177]

Immunocytochemistry/ Immunofluorescence: eIF4E Antibody (5D11) - BSA Free [NBP2-22177]

Immunocytochemistry/Immunofluorescence: eIF4E Antibody (5D11) [NBP2-22177] - Immunofluorescence analysis of GC-7901 cells using eIF4E mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) - BSA Free [NBP2-22177]

Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) - BSA Free [NBP2-22177]

Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) [NBP2-22177] - Immunohistochemical analysis of paraffin-embedded stomach cancer (left) and prostate cancer (right) using eIF4E mouse mAb with DAB staining.
Flow Cytometry: eIF4E Antibody (5D11) - BSA Free [NBP2-22177]

Flow Cytometry: eIF4E Antibody (5D11) - BSA Free [NBP2-22177]

Flow Cytometry: eIF4E Antibody (5D11) [NBP2-22177] - Flow cytometric analysis of Hela cells using EIF4E mouse mAb (green) and negative control (purple).
Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) - BSA Free [NBP2-22177]

Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) - BSA Free [NBP2-22177]

Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) [NBP2-22177] - Immunohistochemical analysis of paraffin-embedded liver cancer (left) and submaxillary tumor (right) using eIF4E mouse mAb with DAB staining.
ELISA: eIF4E Antibody (5D11) - BSA Free [NBP2-22177]

ELISA: eIF4E Antibody (5D11) - BSA Free [NBP2-22177]

ELISA: eIF4E Antibody (5D11) [NBP2-22177] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Applications for eIF4E Antibody (5D11)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF4E

eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.

Long Name

Eukaryotic Translation Initiation Factor 4E

Alternate Names

EIF4EL1, EIF4F

Gene Symbol

EIF4E

Additional eIF4E Products

Product Documents for eIF4E Antibody (5D11)

Certificate of Analysis

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Product Specific Notices for eIF4E Antibody (5D11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Interferons
Translation Regulation

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Protocols

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FAQs

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