Endoglin/CD105 Antibody (MJ7/18) - BSA Free

Novus Biologicals | Catalog # NB100-77666

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # MJ7/18

Format

BSA Free
View all Endoglin/CD105 Primary Antibodies »

Product Specifications

Immunogen

Inflamed mouse skin

Epitope

Endothelial cell selective TGB1 binding molecule-endoglin

Marker

Neo-endothelial Cells Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for Endoglin/CD105 Antibody (MJ7/18) - BSA Free

Western Blot: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666]

Western Blot: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666]

Western Blot: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Total protein from mouse placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocking in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD105 (NB100-77666) in 1% milk/TBST and detected with an anti-rat HRP conjugated secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666]

Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666]

Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Using the DyLight 550 direct conjugate Staining of CD105 (endoglin) in 4T1 tumors after radioimmunotherapy shows CD105 expression (red), blood vessels (green), and nuclei (blue).
Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666]

Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666]

Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Analysis of a FFPE mouse ductus deference using a 1:50 dilution of CD105 antibody (clone MJ7/18) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Staining was observed in the pseudostratified columnar epithelial cells. Staining was performed by Histowiz.
Flow Cytometry: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666]

Flow Cytometry: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666]

Flow Cytometry: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Analysis of Biotin conjugate of NB100-77666. Surface staining of bEND.3 cell line with 0.25 ug of Anti-Mouse CD105 (Endoglin) Biotin followed by Streptavidin PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Flow (Cell Surface): Endoglin/CD105 Antibody (MJ7/18) [NB100-77666]

Flow (Cell Surface): Endoglin/CD105 Antibody (MJ7/18) [NB100-77666]

Flow (Cell Surface): Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - A surface stain was performed on MS-1 Cells with Endoglin/CD105 (MJ7/18) antibody NB100-77666 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).

Applications for Endoglin/CD105 Antibody (MJ7/18) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume. It is recommended that the reagent be titrated for optimal performance for each application.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Endoglin/CD105

CD105, commonly referred to as Endoglin, ENG, HHT1, and ORW1 is a membrane single-pass type I membrane protein limited to endothelial cells in all tissues except bone marrow. It is a major glycoprotein of vascular endothelium and plays a major role in the regulation of angiogenesis. CD105 acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Mutations involving CD105 have been shown to be involved with hereditary hemorrhagic telangiectasia, as well interruptions of the TGF-beta/BMP pathways leading to some cancers. Immunohistochemistry has shown that CD105 has a very high expression in blood vessels of tumors, making it a potential target that can be used in tumor imaging (PMID: 12773481). Elevated serum levels of CD105 in pregnant women have been determined a possible risk factor for preeclampsia (PMID: 24273904).

Alternate Names

CD105, ENG

Gene Symbol

ENG

Additional Endoglin/CD105 Products

Product Documents for Endoglin/CD105 Antibody (MJ7/18) - BSA Free

Certificate of Analysis

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Product Specific Notices for Endoglin/CD105 Antibody (MJ7/18) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Endoglin/CD105 Antibody (MJ7/18) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Endoglin/CD105 Antibody (MJ7/18) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?

    A: We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

  • Q: We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A:

    We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

  • Q: I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?

    A: We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

  • Q: We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A:

    We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

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