EpCAM/TROP1 Antibody (BLR077G)

Novus Biologicals | Catalog # NBP3-14685

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR077G
View all EpCAM/TROP1 Primary Antibodies »

Product Specifications

Immunogen

between 264 and 314 (C-term)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for EpCAM/TROP1 Antibody (BLR077G)

Western Blot: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685]

Western Blot: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685]

Western Blot: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685] - Whole cell lysate (50 ug) from BW5147.3, CTLL-2, EL4, and mIMCD-3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody [BLR077G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/ Immunofluorescence: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685]

Immunocytochemistry/ Immunofluorescence: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685]

Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685] - Section of OVCAR-3 cells. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody [BLR077G] used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685]

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685]

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685] - Section of human breast carcinoma. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody [BLR077G] used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Western Blot: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685]

Western Blot: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685]

Western Blot: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685] - Whole cell lysate (50 ug) from HeLa, MCF-7, K-562, LNCaP, RKO, Hep-G2, Jurkat, HT-29, and SW620 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody [BLR077G]used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with anexposure time of 10 seconds. Lower Panel: Rabbit antiRPL23.
Immunocytochemistry/ Immunofluorescence: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685]

Immunocytochemistry/ Immunofluorescence: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685]

Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685] - Section of HT-29 cells. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody [BLR077G] used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DA
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685]

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685]

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685] - Rabbit anti-EpCAM recombinant monoclonal antibody [BLR077G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI.
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685]

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685]

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685] - Human ovarian carcinoma. Antibody: Rabbit anti-EpCAM recombinant monoclonalantibody [BLR077G] used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG(A120-501P). Substrate: DA
Immunoprecipitation: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685]

Immunoprecipitation: EpCAM/TROP1 Antibody (BLR077G) [NBP3-14685]

Immunoprecipitation: EpCAM/TROP1 Antibody [NBP3-14685] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cellsprepared using NETN lysis buffer. Antibody: Rabbit antiEpCAM recombinant monoclonal antibody used at 12 ul per reaction. For blottingimmunoprecipitated EpCAM was used at1:1000. Chemiluminescence with an exposure time of 30seconds.

Applications for EpCAM/TROP1 Antibody (BLR077G)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 -1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

12 ul/1 mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: EpCAM/TROP1

Epithelial cell adhesion molecule (EpCAM), also known as TROP1 and CD326, is a type I transmembrane glycoprotein that plays a role in a number of cellular processes including adhesion, signaling, maintaining stemness, proliferation, migration, and invasion (1,2). The human EpCAM protein is 314 amino acids (aa) in length with a theoretical molecular weight (MW) of ~35 kDa (1,3,4). The EpCAM protein includes a signal peptide, an extracellular N-terminal domain, a thyroglobulin type-1 domain, a carboxyl-terminal domain, a single-pass transmembrane domain, and an intracellular domain (1,3). The protein is highly conserved and has approximately 81% aa sequence identity between human and mouse (5). Trophoblast cell-surface antigen 2 (TROP2) also shares ~67% aa sequence similarity with EpCAM (1,5). While both EpCAM and TROP2 are cell surface markers expressed in the epithelium, their expression levels typically do not correlate (5). EpCAM is expressed in normal epithelial tissue and elevated expression is observed in many epithelial carcinomas (1-3) Patient tumor samples with increased EpCAM expression have been associated with poor prognosis (1). Given EpCAM's elevated expression in tumors, it has become a potential target for cancer therapy approaches, including monoclonal antibody treatment and anti-EpCAM trispecific antibodies (1,5).

EpCAM functions as an intracellular signaling molecule and contributes to regulation of epithelial-to-mesenchymal (EMT) transition (4,5). EpCAM is cleaved during the process of regulated intramembrane proteolysis (RIP) (4,5). Initial cleavage occurs at the membrane via a disintegrin and metalloprotease 17 (ADAM17) which releases EpCAM's extracellular domain (EpEx) (4,5). A secondary cleavage is mediated by presenilin 2 (PSEN2) which releases EpCAM's cytoplasmic trail (EpICD) (4,5). EpICD translocates to the nucleus where it associates with beta-catenin, FHL-2, and LEF-1 and induces transcription of genes related to EMT and tumor growth (4,5). EpCAM has also been shown to regulate structure and functionality of the apical junction complex of cells through direct interaction with claudin-7 and association with E-cadherin (2,5). Loss of EpCAM disrupts adherins junction and tight junction structure and function (2).

References

1. Mohtar MA, Syafruddin SE, Nasir SN, Low TY. Revisiting the Roles of Pro-Metastatic EpCAM in Cancer. Biomolecules. 2020; 10(2):255. https://doi.org/10.3390/biom10020255

2. Huang L, Yang Y, Yang F, et al. Functions of EpCAM in physiological processes and diseases (Review). Int J Mol Med. 2018; 42(4):1771-1785. https://doi.org/10.3892/ijmm.2018.3764

3. Brown TC, Sankpal NV, Gillanders WE. Functional Implications of the Dynamic Regulation of EpCAM during Epithelial-to-Mesenchymal Transition. Biomolecules. 2021; 11(7):956. https://doi.org/10.3390/biom11070956

4. Uniprot (P16422)

5. Schnell U, Cirulli V, Giepmans BN. EpCAM: structure and function in health and disease. Biochim Biophys Acta. 2013; 1828(8):1989-2001. https://doi.org/10.1016/j.bbamem.2013.04.018

Long Name

Epithelial Cell Adhesion Molecule

Alternate Names

17-1A, CD326, GA733-2, gp40, KS1/4, M4S1, TACSTD1, TROP1

Gene Symbol

EPCAM

Additional EpCAM/TROP1 Products

Product Documents for EpCAM/TROP1 Antibody (BLR077G)

Certificate of Analysis

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Product Specific Notices for EpCAM/TROP1 Antibody (BLR077G)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for EpCAM/TROP1 Antibody (BLR077G)

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  • Q: I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?

    A:

    We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

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