Key Product Details

Species Reactivity

Human, Rat, Chinese Hamster

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

DyLight 755 (Excitation = 754 nm, Emission = 776 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 8F1
View all ERCC1 Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant human ERCC1. [UniProt# P07992]

Reactivity Notes

Human, rat. Chinese Hamster reactivity reported in scientific literature (PMID: 17962301) Not yet tested in other species.

Localization

Nuclear.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for ERCC1 Antibody (8F1) [DyLight 755]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: ERCC1

The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1 XPF complex makes the 5'incision. Elevated levels of ERCC1 have also been reported in Cisplatin resistant cells.

Long Name

Excision Repair Cross-complementing 1

Alternate Names

COFS4, DNA excision repair protein ERCC-1, excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence), RAD10, UV20

Gene Symbol

ERCC1

Additional ERCC1 Products

Product Documents for ERCC1 Antibody (8F1) [DyLight 755]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ERCC1 Antibody (8F1) [DyLight 755]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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FAQs

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