ERCC1 Antibody (8F1) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80725
Key Product Details
Species Reactivity
Human, Rat, Chinese Hamster
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 8F1
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length recombinant human ERCC1. [UniProt# P07992]
Reactivity Notes
Chinese Hamster reactivity reported in scientific literature (PMID: 17962301) Not yet tested in other species.
Localization
Nuclear.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for ERCC1 Antibody (8F1) - Azide and BSA Free
![Immunohistochemistry: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725]](https://resources.rndsystems.com/images/products/ERCC1-Antibody-8F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80725-img0001.jpg "Immunohistochemistry: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725]")
Immunohistochemistry: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725]
Immunohistochemistry: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725] - Analysis of ERCC1 in human non-small cell lung cancer. Image courtesy of product review submitted by Alex Soltermann. Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725]](https://resources.rndsystems.com/images/products/ERCC1-Antibody-8F1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80725-img0002.jpg "Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725]")
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725]
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725] - Formalin fixed paraffin embedded human tonsil stained with ERCC1 antibody. Image from the standard format of this antibody.Applications for ERCC1 Antibody (8F1) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500. Use reported in scientific literature (PMID 21961533)
Western Blot
1:100-1:2000. Use reported in scientific literature
Application Notes
IHC: In an ABC method, we suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ERCC1
Long Name
Excision Repair Cross-complementing 1
Alternate Names
COFS4, DNA excision repair protein ERCC-1, excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence), RAD10, UV20
Gene Symbol
ERCC1
Additional ERCC1 Products
Product Documents for ERCC1 Antibody (8F1) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ERCC1 Antibody (8F1) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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