ERCC1 Antibody (8F1) - BSA Free

Novus Biologicals | Catalog # NB500-704

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Rat, Chinese Hamster

Cited:

Human, Hamster - Cricetulus (Chinese Hamster)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 8F1

Format

BSA Free
View all ERCC1 Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant human ERCC1. [UniProt# P07992]

Reactivity Notes

Human, rat. Chinese Hamster reactivity reported in scientific literature (PMID: 17962301) Not yet tested in other species.

Localization

Nuclear.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for ERCC1 Antibody (8F1) - BSA Free

Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - BSA Free [NB500-704]

Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - BSA Free [NB500-704]

ERCC1-Antibody-8F1-Immunohistochemistry-Paraffin-NB500-704-img0004.jpg
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - BSA Free [NB500-704]

Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - BSA Free [NB500-704]

Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Analysis of ERCC1 in human non-small cell lung cancer. Image courtesy of product review submitted by Alex Soltermann.
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - BSA Free [NB500-704]

Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - BSA Free [NB500-704]

Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Formalin fixed paraffin embedded human tonsil stained with ERCC1 antibody.
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - BSA Free [NB500-704]

Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - BSA Free [NB500-704]

ERCC1-Antibody-8F1-Immunohistochemistry-Paraffin-NB500-704-img0005.jpg
ERCC1 Antibody (8F1) - BSA Free

Immunohistochemistry: ERCC1 Antibody (8F1) - BSA Free [NB500-704] -

Immunohistochemistry: ERCC1 Antibody (8F1) - BSA Free [NB500-704] - Anti-ERCC1 immunocytochemistry on cell block core of malignant pleural mesothelioma, using Mab 8F1 & D-10 with CC1-mono & H2-60 protocols. Arrow: Surrounding non-tumoral cells, including lymphocytes, macrophages & neutrophil granulocytes. Arrowhead: Unspecific plasma membrane staining with D-10. 200 × original magnification. Inset lower left: Staining of intracellular mucin vacuoles of a mucinous adenocarcinoma of unknown origin. 400 × original magnification. Image collected & cropped by CiteAb from the following publication (http://jclinbioinformatics.biomedcentral.com/articles/10.1186/2043-9113…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ERCC1 Antibody (8F1) - BSA Free

Western Blot: ERCC1 Antibody (8F1) - BSA Free [NB500-704] -

DHO hydrophilic extract increases the Single Strand Annealing activity in response to CPT treatment (A) Chromatin enhriched purification of HeLa cells pretreated or not with the DHO extract at 1 mg/ml followed by 1 μM CPT treatment for additional two hours. Cells were then lysed to obtain a soluble (S) and a chromatin-enriched (P, as pellet) fraction. Western blotting was performed to analyse the loading onto chromatin of the Poly (ADP-ribose) polymerase (PARP1) protein, involved in the cell response to DNA damage. Total RPA32 and Lamin A/C were used as controls of the supernatant or the chromatin-enriched fraction, respectively. (B) Chromatin enhriched purification of HeLa cells performed as previously for the analysis of RAD52 chromatin loading. (C) Chromatin enhiched purification of HeLa cells was performed as previously described followed by incubation with ERCC1 antibody. (D) HeLa hprtSAGFP cells were transfected with the plasmid encoding the SceI restriction enzyme followed by incubation with 1 mg/ml of DHO extract or vehicle (DMSO) for 48 hours followed by FACS analysis measurement of GFP levels to calculate %SSA frequency compared with control cells which were set as 100%. Data represent the mean % +/- SD. obtained from three independent experiments. Statistically significant differences are indicated with: ***significant (P < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37409248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ERCC1 Antibody (8F1) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:200

Western Blot

1:100-1:2000
Application Notes
IHC: In an ABC method, we suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Antibody can be used in Immunocytochemistry/Immunoflourescence as reported in the literature (PMID: 21961533)Western Blot was reported in scientific literature.

Reviewed Applications

Read 1 review rated 5 using NB500-704 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERCC1

ERCC1, also known as RAD10, COFS4 and UV20, is a gene that functions in the nucleotide excision repair pathway. Specifically, ERCC1 is required for the repair of DNA lesions induced by UV light or formed by electrophilic compounds. ERCC1 is a heterodimer composed of ERCC1 and XPF/ERRC4 and is located in the nucleus. Mutations in ERCC1 result in cerebrooculofacio skeletal syndrome, as well as carcinogenesis. Recent research on DNA repair suggests that DNA damage may drive accelerated bone aging (PMID: 23281008), and that defaults in DNA repair mechanisms are prevalent in many lung cancers (PMID: 23276504).

Long Name

Excision Repair Cross-complementing 1

Alternate Names

COFS4, DNA excision repair protein ERCC-1, excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence), RAD10, UV20

Entrez Gene IDs

2067 (Human); 292673 (Rat)

Gene Symbol

ERCC1

UniProt

P07992 (Human)

Additional ERCC1 Products

Product Documents for ERCC1 Antibody (8F1) - BSA Free

Certificate of Analysis

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Product Specific Notices for ERCC1 Antibody (8F1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for ERCC1 Antibody (8F1) - BSA Free

Customer Reviews for ERCC1 Antibody (8F1) - BSA Free (1)

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  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Non-small cell lung cancer
    Species: Human
    Verified Customer | Posted 12/14/2011
    ERCC1 Antibody (8F1) - BSA Free NB500-704

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