ExCellerate™ iPSC Expansion Medium, Animal Component-Free
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ExCellerate™ iPSC Expansion Medium, Animal Component-Free SummaryAn animal component-free medium that supports robust expansion and maintenance of pluripotent stem cell culture for enhanced consistency and reproducibility
• Ready-to-use: Complete all-in-one formulation, no growth factor supplementation required
• Animal component-free: Ideal for translational research
• Robust: Superior expansion and maintenance of undifferentiated iPSCs
• Consistent: Reproducible results
• Flexible: Compatible with a variety of matrices and cell lines
• Stable: Maintains cell viability, genotype and differentiation potential over long-term culture
Why Use ExCellerate™ iPSC Expansion Medium?
ExCellerate™ iPSC Expansion Medium is specifically formulated for the in vitro, feeder-free expansion of human pluripotent stem cells, including embryonic stem (ES) and induced pluripotent stem (iPS) cells. The formulation is complete and contains the growth factors required to support iPSC expansion and maintenance in a single, ready-to-use format. This simplifies stem cell culture workflows and provides a reproducible cell culture environment to ensure consistent performance.
No materials of animal or human origin are used in the production of this medium or its components. Elimination of animal-derived components reduces variability and improves lot-to-lot consistency for reliable generation of high quality, homogenous, and undifferentiated cells needed for any stage of research. Animal component-free traceability of raw materials reduces risk and makes it ideal for translational research. An animal-free, GMP grade version is in development to support a seamless transition into clinical applications.
The composition of ExCellerate™ iPSC Expansion Medium is optimized to support the robust expansion of pluripotent stem cells, while maintaining cell viability, genotype and differentiation potential for extended times in vitro. WiCell has concluded that ExCellerate™ iPSC Expansion Medium has met all WiCell requirements for quality and is appropriate for pluripotent stem cell culture.
This medium is compatible with various cell culture matrices, including Cultrex Ultimatrix, Cultrex ReadyBME and Recombinant Human Vitronectin, and cell lines, making it versatile for use in research, preclinical, and translational stem cell culture workflows.
500 mL of ExCellerate iPSC Expansion Media
For research use only. Not for diagnostic use.
Figure 4. HiPSCs cultured in ExCellerate™ iPSC Expansion Medium maintain expression of stemness markers over long-term culture HiPSC lines maintained in ExCellerate™ iPSC Expansion Medium express undifferentiated stem cell markers OCT3/4 (red, Catalog # AF1759) and Tra-1-60 (red, Catalog # MAB4770) along with F-actin (green) and DAPI (blue, Catalog # 5748) (A). HiPSC lines express high levels of OCT3/4, SSEA-4, SOX2, and no SSEA-1 as assessed by flow cytometry (Catalog # FMC001). Undifferentiated stem cell marker expression is greater than 97% across 4 cell lines after more than 45 passages (B-C). Graph is displayed as average ± standard deviation.
Figure 2. Consistent performance with ExCellerate™ iPSC Expansion Medium across cell lines and matrices Different human iPSC lines maintained in ExCellerate™ iPSC Expansion Medium showed minimal line-to-line variability in cumulative fold expansion over 15 days (A) and exhibit greater than ~90% viability after 4 days in culture (B). Graph is displayed as average ± standard deviation. Cell lines were cultured on Cultrex™ UltiMatrix (Catalog # BME001), Recombinant Human Vitronectin (Catalog # 2308-VN).
Figure 6. Lot-to-lot consistency with ExCellerate™ iPSC Expansion Medium. Human iPSC lines cultured in different lots of ExCellerate™ iPSC Expansion Medium express high levels of undifferentiated stem cell markers OCT-3/4, SOX2, SSEA-4 and no SSEA-1 as assessed flow cytometry (Catalog # FMC001).
Figure 1. Superior expansion of hiPSCs with ExCellerate™ iPSC Expansion Medium Human iPSC were maintained in either ExCellerate™ iPSC medium or a competitor’s iPSC medium on different substrates: Recombinant Human Vitronectin (Catalog # 2308-VN) or Cultrex ™ UltiMatrix (Catalog # BME001) for 3 days in 6 well plates. Colonies were then dissociated as single cells and counted to obtain fold expansion. Initial cell seeding density was 50,000 cells per well. Graph is displayed as Average ± SEM.
Figure 7. HiPSCs cultured in ExCellerate™ iPSC Expansion Medium show normal karyotype. HiPSC lines maintained in ExCellerate™ iPSC Expansion Medium for more than 30 passages display normal karyotype.
Figure 3. Human iPSCs cultured in ExCellerate™ iPSC Expansion Medium show characteristics of classic hiPSC colony morphology consistently across cell lines Different hiPSC lines cultured in ExCellerate™ iPSC Expansion Medium show healthy rounded colony morphology and characteristics such as compact cells and clearly defined smooth colony edges.
Figure 5. HiPSCs maintained in ExCellerate™ iPSC Expansion Medium differentiate into cardiomyocytes, hepatocytes and neurons HiPSCs maintain their pluripotency in ExCellerate™ iPSC Expansion Medium and can differentiate into cells of the three germ layers (Catalog # SC027B). Shown here are images of hiPSC derived cardiomyocytes (mesoderm, Catalog # SC032B) expressing cardiac troponin T (cTNT1, red, Catalog # MAB1874), F-actin (green) and DAPI (blue, Catalog # 5748) (A); hepatocytes (endoderm, Catalog # SC033) showing expression of albumin (red, Catalog # MAB1455), HNF4a (green, Catalog # PP-H1415) and DAPI (blue) (B); and neurons (ectoderm, Catalog # SC035) expressing N-cadherin (red), Tuj1 (green) and DAPI (blue)(C).
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