Human/Mouse Oct-3/4 Antibody

Catalog # Availability Size / Price Qty
AF1759
AF1759-SP
Detection of Human and Mouse Oct‑3/4 by Western Blot.
6 Images
Product Details
Citations (73)
FAQs
Supplemental Products
Reviews (3)

Human/Mouse Oct-3/4 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Oct-3/4 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
20 µg/mL
See below
Immunoprecipitation
3 µg/106 cells
NTera‑2 human testicular embryonic carcinoma cell line, see our available Western blot detection antibodies
Chromatin Immunoprecipitation (ChIP)
5 µg/106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Oct-3/4 antibody by Western Blot. View Larger

Detection of Human and Mouse Oct‑3/4 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells and D3 mouse embryonic stem cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Oct-3/4 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Alkaline Phosphatase and Oct‑3/4 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

Alkaline Phosphatase and Oct‑3/4 in BG01V Human Stem Cells. Alkaline phosphatase (ALPL) and Oct-3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Human/Mouse/Rat ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Goat Anti-Human/Mouse Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; Catalog # NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Chromatin Immunoprecipitation (ChIP) Detection of Oct-3/4-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of Oct‑3/4-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Oct-3/4/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thenanogpromoter was detected by standard PCR.

Simple Western Detection of Human Oct-3/4 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Oct‑3/4 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Oct-3/4 at approximately 50 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Detection of Human Oct-3/4 by Western Blot View Larger

Detection of Human Oct-3/4 by Western Blot Knockdown OGT inhibits proliferation and tumorsphere formation of hepatoma cell through reducing eIF4E expression. A, Huh7 and PLC/PRF/5 cells were infected with control shRNA, OGT shRNA2 alone, or with wild‐type eIF4E lentivirus. The cell lysates were harvested for western blotting analysis using indicated antibodies. beta ‐actin expression was served as a loading control. B, Cell proliferation of Huh7 and PLC/PRF/5 cells infected with lentiviruses as in panel (A) were measured with CCK8 assay. (C‐H) Huh7 and PLC/PRF/5 cells infected with lentiviruses as in panel (A) were seeded into 96‐well plates. After 12 d, tumorsphere were counted and quantified. Representative images of sphere (scale bars, 100 μm) were shown (C, F). The diameter of sphere (D, G) and number of sphere (E, H) were count. Data represent mean ± SD of at least three independent experiments. The two‐tailed Student's t tests were used. **P < 0.01. I, Huh7 cells expressing either OGT shRNA2 alone or with wild‐type eIF4E lentivirus were incubated with PE‐labelled anti‐AC133 antibody. The percentages of CD133+ cells in graphs were analysed by flow cytometry. Black line, control IgG staining; red line, CD133 staining. J, Cell lysates were examined by western blotting with indicated antibodies. The right panel showcases relative protein amounts of different groups. Error bars represent ±SD of triplicate experiments. **P < 0.01; n.s, no significance. K, Huh7 cells were collected and subjected to immunoprecipitation with antibody against eIF4E or normal mouse IgG. Total RNAs were purified from immunocomplexes and subjected to RT‐PCR to measure Sox2, OCT4, and KLF4 mRNAs associated with eIF4E Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30677218), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Oct-3/4 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Oct-3/4 by Immunocytochemistry/Immunofluorescence Neural induction of hPSCs. (a) Both hPSC culture and neural differentiation were performed on LN521 substrate. Cortical neurons were induced with dual SMAD inhibition, expanded in the presence of FGF2, and matured with support by a selection of neurotrophic factors. Neural progenitor cells (NPCs) could be cryopreserved at day 21 and plated at day 32 for final experiments, including microelectrode array (MEA) measurements. (b) Three different hPSC lines (one hESC line, 08/023 and two hiPSC lines, 10212.EURCCs and IMR90-4) were characterized for their efficiency in producing neuroectodermal cells in response to 12-day neural induction by dual SMAD inhibition. Cells were stained for pluripotency marker Oct4 at the pluripotent stage and after 12 days of neural induction. The presence of early neuroectodermal markers was evaluated with Sox2, FoxG1 and Pax6 staining. (c) After 21 days of differentiation, the culture contained vimentin- and Pax6-positive NPCs that could be cryopreserved. Additionally, the first Tbr2- and MAP2-positive neurons were detected at this time point. (d) The percentages of Pax6-positive cells were quantified at four time points of differentiation (mean ± s.e.m., n = 5–14, data derived from 1–3 independent differentiations). Statistical analysis was performed with the Mann-Whitney U test to compare differences between day 12 and day 46 within each hPSC line, and significant p-values are presented in the image. The scale bar is 50 µm in all images. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31748598), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Oct-3/4

Oct-3/4, a member of POU transcription factors, was identified as a DNA-binding protein that activates gene transcription via a cis-element containing an octamer motif (1). It is expressed in totipotent embryonic stem and germ cells (2, 3). A critical amount of Oct-3/4 expressed is required to sustain stem cell self-renewal and pluripotency (4). When embryonic stem cells are induced to differentiate, Oct-3/4 is downregulated and this downregulation of Oct-3/4 has proven to be essential for proper and divergent developmental program (5). The Oct-3/4 molecule is not only a master regulator of pluripotency that controls the lineage commitment but also is the most recognized marker used for the identification of totipotent embryonic stem cells.

References
  1. Scholer, H.R. et al. (1990) Nature 344:435.
  2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
  3. Rosner, M.H. et al. (1990) Nature 345:686.
  4. Niwa, H. et al. (2000) Nat. Genet. 24:372.
  5. Pesce, M. et al. (2001) Stem Cells 19:271.
Long Name
Octamer-binding Protein 3/4
Entrez Gene IDs
5460 (Human); 18999 (Mouse)
Alternate Names
3-Oct; Oct-3/4; OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1; Oct4; Otf3g; Otf4; Pou5f1

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Citations for Human/Mouse Oct-3/4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

73 Citations: Showing 1 - 10
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  1. Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip
    Authors: S Musah, N Dimitrakak, DM Camacho, GM Church, DE Ingber
    Nat Protoc, 2018-07-01;13(7):1662-1685.
  2. Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip
    Authors: Musah S, Mammoto A, Ferrante TC et al.
    Nat Biomed Eng.
  3. Prognostic value of stem cell markers in esophageal and esophagogastric junction cancer: a meta-analysis
    Authors: E Trevellin, G Pirozzolo, M Fassan, R Vettor
    J Cancer, 2020-04-27;11(14):4240-4249.
  4. Generation of five induced pluripotent stem cell lines from patients with MECP2 Duplication Syndrome
    Authors: Mendonca, D;Cappuccio, G;Sheppard, J;Delacruz, M;Bengtsson, J;Carvalho, CMB;Bajic, A;Park, H;Kim, JJ;Jafar-Nejad, P;Coquery, C;Pehlivan, D;Suter, B;Maletic-Savatic, M;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Generation of an Alagille syndrome (ALGS) patient-derived induced pluripotent stem cell line (TRNDi032-A) carrying a heterozygous mutation (p.Cys682Leufs*7) in the JAG1 gene
    Authors: Hatim, O;Pavlinov, I;Xu, M;Linask, K;Beers, J;Liu, C;Baumgärtel, K;Gilbert, M;Spinner, N;Chen, C;Zou, J;Zheng, W;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Flow Cytometry
  6. Partial cellular reprogramming stably restores the stemness of senescent epidermal stem cells
    Authors: Su, YR;Gu, SM;Liu, YR;Cheng, YQ;Wan, Q;Sang, X;Chen, MH;Liu, WQ;Shi, Q;Liu, C;Liu, Y;Li, CY;Wang, ZC;Wang, XR;
    European review for medical and pharmacological sciences
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  7. Zinc finger and SCAN domain-containing 18 suppresses the proliferation, self-renewal, and drug resistance of glioblastoma cells
    Authors: Wang, Y;Peng, J;Song, C;Yang, Y;Qin, T;
    Heliyon
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Cynomolgus monkey embryo model captures gastrulation and early pregnancy
    Authors: J Li, Q Zhu, J Cao, Y Liu, Y Lu, Y Sun, Q Li, Y Huang, S Shang, X Bian, C Li, L Zhang, Y Wang, Y Nie, J Fu, W Li, MA Mazid, Y Jiang, W Jia, X Wang, Y Sun, MA Esteban, Q Sun, F Zhou, Z Liu
    Cell Stem Cell, 2023-04-06;30(4):362-377.e7.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Embryo
    Applications: IHC
  9. Generation of three induced Pluripotent Stem Cell lines from individuals with Hypomyelination with Atrophy of Basal Ganglia and Cerebellum caused by a c.745G>A (p.D249N) autosomal dominant mutation in TUBB4A
    Authors: AA Almad, L Garcia, A Takanohash, A Gagne, W Yang, J Ann McGuir, D French, A Vanderver
    Stem Cell Research, 2023-03-26;69(0):103083.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  10. Cell-type dependent regulation of pluripotency and chromatin remodeling genes by hydralazine
    Authors: Alain Aguirre-Vázquez, Fabiola Castorena-Torres, Beatriz Silva-Ramírez, Katia Peñuelas-Urquides, María Elena Camacho-Moll, Luis A. Salazar-Olivo et al.
    Stem Cell Research & Therapy
  11. Epiblast-like stem cells established by Wnt/ beta -catenin signaling manifest distinct features of formative pluripotency and germline competence
    Authors: Qing Luo, Han-pin Pui, Jiayu Chen, Leqian Yu, Paulo R. Jannig, Yu Pei et al.
    Cell Reports
  12. Niche stiffness sustains cancer stemness via TAZ and NANOG phase separation
    Authors: X Liu, Y Ye, L Zhu, X Xiao, B Zhou, Y Gu, H Si, H Liang, M Liu, J Li, Q Jiang, J Li, S Yu, R Ma, S Su, JY Liao, Q Zhao
    Nature Communications, 2023-01-16;14(1):238.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. ZFP462 safeguards neural lineage specification by targeting G9A/GLP-mediated heterochromatin to silence enhancers
    Authors: R Yelagandul, K Stecher, M Novatchkov, L Michetti, G Michlits, J Wang, P Hofbauer, G Vainorius, C Pribitzer, L Isbel, S Mendjan, D Schübeler, U Elling, J Brennecke, O Bell
    Nature Cell Biology, 2023-01-05;0(0):.
    Species: Mouse
    Sample Types: Chromatin
    Applications: ChIP
  14. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice
    Authors: O'Shea TM, Ao Y, Wang S et al.
    Nature Communications
  15. Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient
    Authors: J Lotila, T Hyvärinen, H Skottman, L Airas, S Narkilahti, S Hagman
    Oncogene, 2022-07-11;63(0):102865.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  16. In�vitro attachment and symmetry breaking of a human embryo model assembled from primed embryonic stem cells
    Authors: M Simunovic, ED Siggia, AH Brivanlou
    Cell Stem Cell, 2022-06-02;29(6):962-972.e4.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. CRIPTO Is a Marker of Chemotherapy-Induced Stem Cell Expansion in Non-Small Cell Lung Cancer
    Authors: Federica Francescangeli, Maria Laura De Angelis, Rachele Rossi, Giovanni Sette, Adriana Eramo, Alessandra Boe et al.
    Frontiers in Oncology
  18. Oct4 differentially regulates chromatin opening and enhancer transcription in pluripotent stem cells
    Authors: L Xiong, EA Tolen, J Choi, S Velychko, L Caizzi, T Velychko, K Adachi, CM MacCarthy, M Lidschreib, P Cramer, HR Schöler
    Elife, 2022-05-27;11(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  19. Glioblastoma Embryonic-like Stem Cells Exhibit Immune-Evasive Phenotype
    Authors: Borja Sesé, Sandra Íñiguez-Muñoz, Miquel Ensenyat-Mendez, Pere Llinàs-Arias, Guillem Ramis, Javier I. J. Orozco et al.
    Cancers (Basel)
  20. Mesenchymal stem cells derived from human induced pluripotent stem cells improve the engraftment of myogenic cells by secreting urokinase-type plasminogen activator receptor (uPAR)
    Authors: Ahmed Elhussieny, Ken’ichiro Nogami, Fusako Sakai-Takemura, Yusuke Maruyama, Natsumi Takemura, Wael Talaat Soliman et al.
    Stem Cell Research & Therapy
  21. Inability to switch from ARID1A-BAF to ARID1B-BAF impairs exit from pluripotency and commitment towards neural crest formation in ARID1B-related neurodevelopmental disorders
    Authors: L Pagliaroli, P Porazzi, AT Curtis, C Scopa, HMM Mikkers, C Freund, L Daxinger, S Deliard, SA Welsh, S Offley, CA Ott, B Calabretta, SA Brugmann, GWE Santen, M Trizzino
    Nature Communications, 2021-11-09;12(1):6469.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. Human iPSC lines from a Christianson syndrome patient with NHE6 W523X mutation, a biologically-related control, and CRISPR/Cas9 gene-corrected isogenic controls
    Authors: L Ma, M Schmidt, EM Morrow
    Stem Cell Research, 2021-06-18;54(0):102435.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. Neural differentiation medium for human pluripotent stem cells to model physiological glucose levels in human brain
    Authors: ME Mor, A Harvey, M Familari, M St Clair-G, S Viventi, RU de Iongh, FJ Cameron, M Dottori
    Brain research bulletin, 2021-05-19;173(0):141-149.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. Comparative evaluation of isogenic mesodermal and ectomesodermal chondrocytes from human iPSCs for cartilage regeneration
    Authors: MS Lee, MJ Stebbins, H Jiao, HC Huang, EM Leiferman, BE Walczak, SP Palecek, EV Shusta, WJ Li
    Science Advances, 2021-05-19;7(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Directed Differentiation of Human Pluripotent Stem Cells towards Corneal Endothelial-Like Cells under Defined Conditions
    Authors: P Grönroos, T Ilmarinen, H Skottman
    Cells, 2021-02-05;10(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity
    Authors: SK Sarvestani, S Signs, B Hu, Y Yeu, H Feng, Y Ni, DR Hill, RC Fisher, S Ferrandon, RK DeHaan, J Stiene, M Cruise, TH Hwang, X Shen, JR Spence, EH Huang
    Nature Communications, 2021-01-11;12(1):262.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation
    Authors: NQ Liu, M Maresca, T van den Br, L Braccioli, MMGA Schijns, H Teunissen, BG Bruneau, EP Nora, E de Wit
    Nature Genetics, 2020-12-14;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  28. A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells
    Authors: DZ Hazelbaker, A Beccard, G Angelini, P Mazzucato, A Messana, D Lam, K Eggan, LE Barrett
    Sci Rep, 2020-01-20;10(1):635.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  29. Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures
    Authors: T Hyvärinen, A Hyysalo, FE Kapucu, L Aarnos, A Vinogradov, SJ Eglen, L Ylä-Outine, S Narkilahti
    Sci Rep, 2019-11-20;9(1):17125.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  30. Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A
    Authors: F Vlahova, KE Hawkins, AM Ranzoni, KL Hau, R Sagar, P Coppi, AL David, J Adjaye, PV Guillot
    Sci Rep, 2019-05-31;9(1):8126.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  31. Verification and rectification of cell type-specific splicing of a Seckel syndrome-associated ATR mutation using iPS cell model
    Authors: Jose Ichisima, Naoya M. Suzuki, Bumpei Samata, Tomonari Awaya, Jun Takahashi, Masatoshi Hagiwara et al.
    Journal of Human Genetics
  32. SOX2 and SOX9 are markers of clinically aggressive disease in metastatic high-grade serous carcinoma
    Authors: M Sherman-Sa, H Onallah, A Holth, R Reich, B Davidson
    Gynecol. Oncol., 2019-03-21;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  33. O-GlcNAc transferase activates stem-like cell potential in hepatocarcinoma through O-GlcNAcylation of eukaryotic initiation factor 4E
    Authors: B Cao, M Duan, Y Xing, C Liu, F Yang, Y Li, T Yang, Y Wei, Q Gao, J Jiang
    J. Cell. Mol. Med., 2019-01-24;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation
    Authors: W Zhang, C Chronis, X Chen, H Zhang, R Spalinskas, M Pardo, L Chen, G Wu, Z Zhu, Y Yu, L Yu, J Choudhary, J Nichols, MM Parast, B Greber, P Sahlén, K Plath
    Cell Stem Cell, 2019-01-03;24(1):138-152.e8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  35. Small non-coding RNA landscape of extracellular vesicles from human stem cells
    Authors: S Kaur, AG Abu-Shahba, RO Paananen, H Hongisto, H Hiidenmaa, H Skottman, R Seppänen-K, B Mannerströ
    Sci Rep, 2018-10-19;8(1):15503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation, Differentiation
  36. Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa
    Authors: A Buskin, L Zhu, V Chichagova, B Basu, S Mozaffari-, D Dolan, A Droop, J Collin, R Bronstein, S Mehrotra, M Farkas, G Hilgen, K White, KT Pan, A Treumann, D Hallam, K Bialas, G Chung, C Mellough, Y Ding, N Krasnogor, S Przyborski, S Zwolinski, J Al-Aama, S Alharthi, Y Xu, G Wheway, K Szymanska, M McKibbin, CF Inglehearn, DJ Elliott, S Lindsay, RR Ali, DH Steel, L Armstrong, E Sernagor, H Urlaub, E Pierce, R Lührmann, SN Grellschei, CA Johnson, M Lako
    Nat Commun, 2018-10-12;9(1):4234.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  37. Transcriptomic and epigenomic differences in human induced pluripotent stem cells generated from six reprogramming methods
    Authors: Jared M. Churko, Jaecheol Lee, Mohamed Ameen, Mingxia Gu, Meenakshi Venkatasubramanian, Sebastian Diecke et al.
    Nature Biomedical Engineering
  38. Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel
    Authors: X Gao, JJ Yourick, RL Sprando
    Stem Cell Res, 2018-07-27;31(0):193-196.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  39. Modeling Short QT Syndrome Using Human‐Induced Pluripotent Stem Cell–Derived Cardiomyocytes
    Authors: Ibrahim El‐Battrawy, Huan Lan, Lukas Cyganek, Zhihan Zhao, Xin Li, Fanis Buljubasic et al.
    Journal of the American Heart Association
  40. Impact of cytosine methylation on DNA binding specificities of human transcription factors
    Authors: Yimeng Yin, Ekaterina Morgunova, Arttu Jolma, Eevi Kaasinen, Biswajyoti Sahu, Syed Khund-Sayeed et al.
    Science
  41. Dinaciclib potently suppresses MCL-1 and selectively induces the cell death in human iPS cells without affecting the viability of cardiac tissue
    Authors: K Alsayegh, K Matsuura, H Sekine, T Shimizu
    Sci Rep, 2017-03-31;7(0):45577.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  42. Brain tissue banking for stem cells for our future
    Sci Rep, 2016-12-19;6(0):39394.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: IHC
  43. Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9
    Stem Cell Res, 2016-09-16;17(2):430-432.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  44. Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9
    Stem Cell Res, 2016-09-15;17(2):441-443.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  45. Derivation of induced pluripotent stem cells from orangutan skin fibroblasts
    Authors: Krishna Ramaswamy, Wing Yan Yik, Xiao-Ming Wang, Erin N. Oliphant, Wange Lu, Darryl Shibata et al.
    BMC Research Notes
  46. Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells.
    Authors: Wada, Naohisa, Maeda, Hidefumi, Hasegawa, Daigaku, Gronthos, Stan, Bartold, Peter Ma, Menicanin, Danijela, Fujii, Shinsuke, Yoshida, Shinichi, Tomokiyo, Atsushi, Monnouchi, Satoshi, Akamine, Akifumi
    Stem Cells Dev, 2014-02-10;23(18):2225-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  47. Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells.
    Authors: Luo, Lan, Kawakatsu, Miho, Guo, Chao-Wan, Urata, Yoshishi, Huang, Wen-Jing, Ali, Haytham, Doi, Hanako, Kitajima, Yuriko, Tanaka, Takayuki, Goto, Shinji, Ono, Yusuke, Xin, Hong-Bo, Hamano, Kimikazu, Li, Tao-Shen
    Sci Rep, 2014-01-21;4(0):3779.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  48. Pluripotent stem cells derived from mouse and human white mature adipocytes.
    Authors: Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K
    Stem Cells Transl Med, 2014-01-06;3(2):161-71.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  49. Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming.
    Authors: Apostolou E, Ferrari F, Walsh R, Bar-Nur O, Stadtfeld M, Cheloufi S, Stuart H, Polo J, Ohsumi T, Borowsky M, Kharchenko P, Park P, Hochedlinger K
    Cell Stem Cell, 2013-05-09;12(6):699-712.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP, Co-Immunoprecipitation
  50. The Pluripotency Factor-Bound Intron 1 of Xist Is Dispensable for X Chromosome Inactivation and Reactivation In Vitro and In Vivo
    Authors: Alissa Minkovsky, Tahsin Stefan Barakat, Nadia Sellami, Mark Henry Chin, Nilhan Gunhanlar, Joost Gribnau et al.
    Cell Reports
  51. Identification of Human Embryonic Progenitor Cell Targeting Peptides Using Phage Display
    Authors: Paola A. Bignone, Rachel A. Krupa, Hal Sternberg, Walter D. Funk, Evan Y. Snyder, Michael D. West et al.
    PLoS ONE
  52. Ex Vivo Reconstitution of Arterial Endothelium by Embryonic Stem Cell-Derived Endothelial Progenitor Cells in Baboons
    Authors: Qiang Shi, Vida Hodara, Calvin R. Simerly, Gerald P. Schatten, John L. VandeBerg
    Stem Cells and Development
  53. Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
    Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji
    PLoS ONE, 2012-09-24;7(9):e45435.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  54. Reduced PLP1 expression in induced pluripotent stem cells derived from a Pelizaeus–Merzbacher disease patient with a partial PLP1 duplication
    Authors: Keiko Shimojima, Takahito Inoue, Yuki Imai, Yasuhiro Arai, Yuta Komoike, Midori Sugawara et al.
    Journal of Human Genetics
  55. Expression of OCT4 in human esophageal squamous cell carcinoma is significantly associated with poorer prognosis
    Authors: Wei He, Ke Li, Feng Wang, Yan-Ru Qin, Qing-Xia Fan
    World Journal of Gastroenterology
  56. Human Embryonic Stem Cells Express Elevated Levels of Multiple Pro-Apoptotic BCL-2 Family Members
    Authors: David T. Madden, Diana Davila-Kruger, Simon Melov, Dale E. Bredesen
    PLoS ONE
  57. A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells.
    Authors: Wong RC, Pollan S, Fong H, Ibrahim A, Smith EL, Ho M, Laslett AL, Donovan PJ
    Stem Cells, 2011-10-01;29(10):1517-27.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  58. Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
    Authors: Kubota H, Wu X, Goodyear SM, Avarbock MR, Brinster RL
    FASEB J., 2011-04-27;25(0):2604-14.
    Species: Rabbit
    Sample Types: Whole Cells
    Applications: ICC
  59. Hydroxyurea-induced global transcriptional suppression in mouse ES cells
    Authors: Peng Cui, Qiang Lin, Chengqi Xin, Lu Han, Lili An, Yulan Wang et al.
    Carcinogenesis
  60. The POU Domain Transcription Factor POU3F1 Is an Important Intrinsic Regulator of GDNF-Induced Survival and Self-Renewal of Mouse Spermatogonial Stem Cells1
    Authors: Xin Wu, Jon M. Oatley, Melissa J. Oatley, Amy V. Kaucher, Mary R. Avarbock, Ralph L. Brinster
    Biology of Reproduction
  61. SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells.
    Authors: Chng Z, Teo A, Pedersen RA, Vallier L
    Cell Stem Cell, 2010-01-08;6(1):59-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  62. Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
    Authors: Fong H, Hohenstein KA, Donovan PJ
    Stem Cells, 2008-04-03;26(8):1931-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  63. Human embryonic and neuronal stem cell markers in retinoblastoma
    Authors: Gail M. Seigel, Abigail S. Hackam, Arupa Ganguly, Lorrie M. Mandell, Federico Gonzalez-Fernandez
    Mol Vis
  64. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
    Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
    Stem Cells, 2007-02-01;25(2):490-9.
    Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  65. A comparison of NIH-approved human ESC lines.
    Authors: Ware CB, Nelson AM, Blau CA
    Stem Cells, 2006-08-17;24(12):2677-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  66. Controlled-rate freezing of human ES cells.
    Authors: Ware CB, Nelson AM, Blau CA
    BioTechniques, 2005-06-01;38(6):879-80, 882-3.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  67. Establishment of a developmental toxicity assay based on human iPSC reporter to detect FGF signal disruption
    Authors: Seiya Kanno, Yusuke Okubo, Tatsuto Kageyama, Lei Yan, Satoshi Kitajima, Junji Fukuda
    iScience
  68. Human Early Syncytiotrophoblasts Are Highly Susceptible to SARS-CoV-2 Infection
    Authors: Ruan D, Ye Z, Yuan S et al.
    Cell Reports Medicine
  69. Induced hepatic stem cells are suitable for human hepatocyte production
    Authors: Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara et al.
    iScience
  70. Cooperative Binding of Transcription Factors Orchestrates Reprogramming
    Authors: Chronis C, Fiziev P, Papp B et al.
    Cell
  71. Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors: In Vitro and In Vivo Post-grafting Safety Characterization
    Authors: M Shigyo, Y Kobayashi, O Platoshyn, S Marsala, T Kato, N Takamura, K Yoshida, A Kishino, M Bravo-Hern, S Juhas, J Juhasova, H Studenovsk, V Proks, JD Ciacci, M Marsala
    Cell Transplantation, 2023-01-01;32(0):9636897231163.
  72. Prostate Cancer Cell Lines under Hypoxia Exhibit Greater Stem-Like Properties.
    Authors: Ma Y, Liang D, Liu J et al.
    PLoS ONE 6(12).

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Human/Mouse Oct-3/4 Antibody
By Anonymous on 10/24/2023
Application: Immunocytochemistry/Immunofluorescence Sample Tested: human induced pluripotent stem cells Species: Human

Oct4 staining performed on iPSCs.


Human/Mouse Oct-3/4 Antibody
By Shuba Krishnan on 10/18/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: iPS2 human induced pluripotent stem cells Species: Human

Cells: human induced pluripotent stem cells
Primary antibody: Goat anti-human OCT3/4 (1:200), O/N incubation at 4 degrees
Secondary antibody: anti-goat AF568


Human/Mouse Oct-3/4 Antibody
By Anonymous on 11/23/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Umbilical vein endothelial cells Species: Human