Human/Mouse Oct-3/4 Antibody

Catalog # Availability Size / Price Qty
AF1759
AF1759-SP
Detection of Human and Mouse Oct‑3/4 by Western Blot.
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Product Details
Citations (36)
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Reviews (2)

Human/Mouse Oct-3/4 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Oct-3/4 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
20 µg/mL
See below
Immunoprecipitation
3 µg/106 cells
NTera‑2 human testicular embryonic carcinoma cell line, see our available Western blot detection antibodies
Chromatin Immunoprecipitation (ChIP)
5 µg/106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Oct-3/4 antibody by Western Blot. View Larger

Detection of Human and Mouse Oct‑3/4 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells and D3 mouse embryonic stem cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Oct-3/4 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chromatin Immunoprecipitation (ChIP) Detection of Oct-3/4-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of Oct‑3/4-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Oct-3/4/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thenanogpromoter was detected by standard PCR.

Immunocytochemistry Alkaline Phosphatase and Oct‑3/4 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

Alkaline Phosphatase and Oct‑3/4 in BG01V Human Stem Cells. Alkaline phosphatase (ALPL) and Oct-3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Human/Mouse/Rat ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Goat Anti-Human/Mouse Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; Catalog # NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human Oct-3/4 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Oct‑3/4 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Oct-3/4 at approximately 50 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Oct-3/4

Oct-3/4, a member of POU transcription factors, was identified as a DNA-binding protein that activates gene transcription via a cis-element containing an octamer motif (1). It is expressed in totipotent embryonic stem and germ cells (2, 3). A critical amount of Oct-3/4 expressed is required to sustain stem cell self-renewal and pluripotency (4). When embryonic stem cells are induced to differentiate, Oct-3/4 is downregulated and this downregulation of Oct-3/4 has proven to be essential for proper and divergent developmental program (5). The Oct-3/4 molecule is not only a master regulator of pluripotency that controls the lineage commitment but also is the most recognized marker used for the identification of totipotent embryonic stem cells.

References
  1. Scholer, H.R. et al. (1990) Nature 344:435.
  2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
  3. Rosner, M.H. et al. (1990) Nature 345:686.
  4. Niwa, H. et al. (2000) Nat. Genet. 24:372.
  5. Pesce, M. et al. (2001) Stem Cells 19:271.
Long Name
Octamer-binding Protein 3/4
Entrez Gene IDs
5460 (Human); 18999 (Mouse)
Alternate Names
3-Oct; Oct-3/4; Oct4; Otf3g; Otf4; Pou5f1

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Citations for Human/Mouse Oct-3/4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient
    Authors: J Lotila, T Hyvärinen, H Skottman, L Airas, S Narkilahti, S Hagman
    Oncogene, 2022;63(0):102865.
  2. In�vitro attachment and symmetry breaking of a human embryo model assembled from primed embryonic stem cells
    Authors: M Simunovic, ED Siggia, AH Brivanlou
    Cell Stem Cell, 2022;29(6):962-972.e4.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Oct4 differentially regulates chromatin opening and enhancer transcription in pluripotent stem cells
    Authors: L Xiong, EA Tolen, J Choi, S Velychko, L Caizzi, T Velychko, K Adachi, CM MacCarthy, M Lidschreib, P Cramer, HR Schöler
    Elife, 2022;11(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  4. Comparative evaluation of isogenic mesodermal and ectomesodermal chondrocytes from human iPSCs for cartilage regeneration
    Authors: MS Lee, MJ Stebbins, H Jiao, HC Huang, EM Leiferman, BE Walczak, SP Palecek, EV Shusta, WJ Li
    Science Advances, 2021;7(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Human iPSC lines from a Christianson syndrome patient with NHE6 W523X mutation, a biologically-related control, and CRISPR/Cas9 gene-corrected isogenic controls
    Authors: L Ma, M Schmidt, EM Morrow
    Stem Cell Research, 2021;54(0):102435.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Directed Differentiation of Human Pluripotent Stem Cells towards Corneal Endothelial-Like Cells under Defined Conditions
    Authors: P Grönroos, T Ilmarinen, H Skottman
    Cells, 2021;10(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Neural differentiation medium for human pluripotent stem cells to model physiological glucose levels in human brain
    Authors: ME Mor, A Harvey, M Familari, M St Clair-G, S Viventi, RU de Iongh, FJ Cameron, M Dottori
    Brain research bulletin, 2021;173(0):141-149.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Inability to switch from ARID1A-BAF to ARID1B-BAF impairs exit from pluripotency and commitment towards neural crest formation in ARID1B-related neurodevelopmental disorders
    Authors: L Pagliaroli, P Porazzi, AT Curtis, C Scopa, HMM Mikkers, C Freund, L Daxinger, S Deliard, SA Welsh, S Offley, CA Ott, B Calabretta, SA Brugmann, GWE Santen, M Trizzino
    Nature Communications, 2021;12(1):6469.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity
    Authors: SK Sarvestani, S Signs, B Hu, Y Yeu, H Feng, Y Ni, DR Hill, RC Fisher, S Ferrandon, RK DeHaan, J Stiene, M Cruise, TH Hwang, X Shen, JR Spence, EH Huang
    Nature Communications, 2021;12(1):262.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation
    Authors: NQ Liu, M Maresca, T van den Br, L Braccioli, MMGA Schijns, H Teunissen, BG Bruneau, EP Nora, E de Wit
    Nature Genetics, 2020;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  11. A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells
    Authors: DZ Hazelbaker, A Beccard, G Angelini, P Mazzucato, A Messana, D Lam, K Eggan, LE Barrett
    Sci Rep, 2020;10(1):635.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. O-GlcNAc transferase activates stem-like cell potential in hepatocarcinoma through O-GlcNAcylation of eukaryotic initiation factor 4E
    Authors: B Cao, M Duan, Y Xing, C Liu, F Yang, Y Li, T Yang, Y Wei, Q Gao, J Jiang
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A
    Authors: F Vlahova, KE Hawkins, AM Ranzoni, KL Hau, R Sagar, P Coppi, AL David, J Adjaye, PV Guillot
    Sci Rep, 2019;9(1):8126.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures
    Authors: T Hyvärinen, A Hyysalo, FE Kapucu, L Aarnos, A Vinogradov, SJ Eglen, L Ylä-Outine, S Narkilahti
    Sci Rep, 2019;9(1):17125.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  15. The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation
    Authors: W Zhang, C Chronis, X Chen, H Zhang, R Spalinskas, M Pardo, L Chen, G Wu, Z Zhu, Y Yu, L Yu, J Choudhary, J Nichols, MM Parast, B Greber, P Sahlén, K Plath
    Cell Stem Cell, 2019;24(1):138-152.e8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. SOX2 and SOX9 are markers of clinically aggressive disease in metastatic high-grade serous carcinoma
    Authors: M Sherman-Sa, H Onallah, A Holth, R Reich, B Davidson
    Gynecol. Oncol., 2019;0(0):.
    Species: Human
    Sample Types: Tumor Tissue Fluid
    Applications: ICC
  17. Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa
    Authors: A Buskin, L Zhu, V Chichagova, B Basu, S Mozaffari-, D Dolan, A Droop, J Collin, R Bronstein, S Mehrotra, M Farkas, G Hilgen, K White, KT Pan, A Treumann, D Hallam, K Bialas, G Chung, C Mellough, Y Ding, N Krasnogor, S Przyborski, S Zwolinski, J Al-Aama, S Alharthi, Y Xu, G Wheway, K Szymanska, M McKibbin, CF Inglehearn, DJ Elliott, S Lindsay, RR Ali, DH Steel, L Armstrong, E Sernagor, H Urlaub, E Pierce, R Lührmann, SN Grellschei, CA Johnson, M Lako
    Nat Commun, 2018;9(1):4234.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Small non-coding RNA landscape of extracellular vesicles from human stem cells
    Authors: S Kaur, AG Abu-Shahba, RO Paananen, H Hongisto, H Hiidenmaa, H Skottman, R Seppänen-K, B Mannerströ
    Sci Rep, 2018;8(1):15503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation
  19. Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel
    Authors: X Gao, JJ Yourick, RL Sprando
    Stem Cell Res, 2018;31(0):193-196.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. Dinaciclib potently suppresses MCL-1 and selectively induces the cell death in human iPS cells without affecting the viability of cardiac tissue
    Authors: K Alsayegh, K Matsuura, H Sekine, T Shimizu
    Sci Rep, 2017;7(0):45577.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9
    Stem Cell Res, 2016;17(2):441-443.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  22. Brain tissue banking for stem cells for our future
    Sci Rep, 2016;6(0):39394.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: IHC
  23. Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9
    Stem Cell Res, 2016;17(2):430-432.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  24. Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells.
    Authors: Wada, Naohisa, Maeda, Hidefumi, Hasegawa, Daigaku, Gronthos, Stan, Bartold, Peter Ma, Menicanin, Danijela, Fujii, Shinsuke, Yoshida, Shinichi, Tomokiyo, Atsushi, Monnouchi, Satoshi, Akamine, Akifumi
    Stem Cells Dev, 2014;23(18):2225-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. Pluripotent stem cells derived from mouse and human white mature adipocytes.
    Authors: Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K
    Stem Cells Transl Med, 2014;3(2):161-71.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  26. Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells.
    Authors: Luo, Lan, Kawakatsu, Miho, Guo, Chao-Wan, Urata, Yoshishi, Huang, Wen-Jing, Ali, Haytham, Doi, Hanako, Kitajima, Yuriko, Tanaka, Takayuki, Goto, Shinji, Ono, Yusuke, Xin, Hong-Bo, Hamano, Kimikazu, Li, Tao-Shen
    Sci Rep, 2014;4(0):3779.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming.
    Authors: Apostolou E, Ferrari F, Walsh R, Bar-Nur O, Stadtfeld M, Cheloufi S, Stuart H, Polo J, Ohsumi T, Borowsky M, Kharchenko P, Park P, Hochedlinger K
    Cell Stem Cell, 2013;12(6):699-712.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  28. Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
    Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji
    PLoS ONE, 2012;7(9):e45435.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  29. A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells.
    Authors: Wong RC, Pollan S, Fong H, Ibrahim A, Smith EL, Ho M, Laslett AL, Donovan PJ
    Stem Cells, 2011;29(10):1517-27.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  30. Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
    Authors: Kubota H, Wu X, Goodyear SM, Avarbock MR, Brinster RL
    FASEB J., 2011;25(0):2604-14.
    Species: Rabbit
    Sample Types: Whole Cells
    Applications: ICC
  31. SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells.
    Authors: Chng Z, Teo A, Pedersen RA, Vallier L
    Cell Stem Cell, 2010;6(1):59-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  32. Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
    Authors: Fong H, Hohenstein KA, Donovan PJ
    Stem Cells, 2008;26(8):1931-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  33. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
    Authors: Rajesh&lt;/LastName&gt;&lt;ForeNam D&lt;/Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
    Stem Cells, 2007;25(2):490-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. A comparison of NIH-approved human ESC lines.
    Authors: Ware CB, Nelson AM, Blau CA
    Stem Cells, 2006;24(12):2677-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  35. Controlled-rate freezing of human ES cells.
    Authors: Ware CB, Nelson AM, Blau CA
    BioTechniques, 2005;38(6):879-80, 882-3.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human/Mouse Oct-3/4 Antibody
By Shuba Krishnan on 10/18/2018
Application: ICC/IF Sample Tested: iPS2 human induced pluripotent stem cells Species: Human

Cells: human induced pluripotent stem cells
Primary antibody: Goat anti-human OCT3/4 (1:200), O/N incubation at 4 degrees
Secondary antibody: anti-goat AF568


Human/Mouse Oct-3/4 Antibody
By Anonymous on 11/23/2017
Application: ICC/IF Sample Tested: Umbilical vein endothelial cells Species: Human