Feline IL-5 Antibody (604308) [Biotin]

Name: Feline IL-5 Antibody (604308) [Biotin]
Brand: R&D Systems
SKU: BAM17951
Price: 559 USD
Availability: InStock

R&D Systems | Catalog # BAM17951

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Product Specifications
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Feline

Applications

ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ Clone # 604308

View all IL-5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant feline IL-5
Ile20-Ser134
Accession # O77515

Specificity

Detects feline IL-5 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant feline GM-CSF, recombinant equine, canine, mouse, human, or porcine IL-5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Feline IL-5 Antibody (604308) [Biotin]

Feline IL-5 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:

Capture Reagent: Feline IL‑5 Antibody (Catalog # MAB1795)
Standard: Recombinant Feline IL-5 Protein (Catalog # 1795-FL)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-5

Interleukin-5 (IL-5) is a 40 kDa secreted, heparin-binding, disulfide-linked homodimeric glycoprotein that belongs to the alpha -helical group of cytokines. It promotes the proliferation, differentiation and activation of eosinophils. The 115 amino acid (aa) mature feline IL-5 shares 70% and 59% aa sequence identity with human and mouse IL-5, respectively. The IL-5 receptor is a complex of an IL-5-specific ligand-binding alpha subunit with a signal transducing common beta subunit ( beta c) that is shared with receptor complexes for IL-3 and GM-CSF.