Key Product Details

Species Reactivity

Feline

Applications

ELISA Capture (Matched Antibody Pair), Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 604312
View all IL-5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant feline IL-5
Ile20-Ser134
Accession # O77515

Specificity

Detects feline IL-5 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant feline GM-CSF, recombinant equine, canine, mouse, human, or porcine IL-5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Feline IL‑5 Antibody

Cell Proliferation Induced by IL‑5 and Neutralization by Feline IL‑5 Antibody.

Cell Proliferation Induced by IL‑5 and Neutralization by Feline IL‑5 Antibody.

Recombinant Feline IL-5 (Catalog # 1795-FL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-5 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Feline IL-5 Monoclonal Antibody (Catalog # MAB1795). The ND50 is typically 0.1-0.5 µg/mL.

Applications for Feline IL‑5 Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 30 ng/mL Recombinant Feline IL‑5.

Feline IL-5 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Feline IL‑5 Biotinylated Antibody (Catalog # BAM17951)
  • Standard: Recombinant Feline IL-5 Protein (Catalog # 1795-FL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-5

Interleukin-5 (IL-5) is a 40 kDa secreted, heparin-binding, disulfide-linked homodimeric glycoprotein that belongs to the alpha -helical group of cytokines. It promotes the proliferation, differentiation and activation of eosinophils. The 115 amino acid (aa) mature feline IL-5 shares 70% and 59% aa sequence identity with human and mouse IL-5, respectively. The IL-5 receptor is a complex of an IL-5-specific ligand-binding alpha subunit with a signal transducing common beta subunit ( beta c) that is shared with receptor complexes for IL-3 and GM-CSF.

 

Long Name

Interleukin 5

Alternate Names

BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF

Entrez Gene IDs

3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 100034199 (Equine); 493803 (Feline)

Gene Symbol

IL5

UniProt

O77515

Additional IL-5 Products

Product Documents for Feline IL‑5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Feline IL‑5 Antibody

For research use only

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