Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FGF-4 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of the human FGF4. The immunogen is located within the last 50 amino acids of FGF4. Amino Acid Squence: SKNGKTKKGNRVSPTMK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit FGF-4 Antibody - BSA Free (NBP1-76865) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FGF-4 Antibody - BSA Free

Western Blot: FGF-4 AntibodyBSA Free [NBP1-76865]

Western Blot: FGF-4 AntibodyBSA Free [NBP1-76865]

Western Blot: FGF-4 Antibody [NBP1-76865] - 3T3 cell lysate with FGF4 antibody at (A) 0.5 and (B) 1 ug/mL.
FGF-4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FGF-4 Antibody - BSA Free [NBP1-76865] -

Immunocytochemistry/ Immunofluorescence: FGF-4 Antibody - BSA Free [NBP1-76865] - Immunofluorescence of FGF-4 in 3T3 cells with FGF-4 antibody at 2.5 ug/mL.
FGF-4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FGF-4 Antibody - BSA Free [NBP1-76865] -

Immunocytochemistry/ Immunofluorescence: FGF-4 Antibody - BSA Free [NBP1-76865] - Immunocytochemistry of FGF-4 in 3T3 cells with FGF-4 antibody at 2.5 ug/mL.

Applications for FGF-4 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2.5 ug/ml

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGF-4

Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.

Long Name

Fibroblast Growth Factor 4

Alternate Names

FGF4, HBGF-4, HST-1, KFGF, KS3

Gene Symbol

FGF4

UniProt

P08620

Additional FGF-4 Products

Product Documents for FGF-4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FGF-4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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