Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FIZZ1/RELM alpha Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to amino acids sequence within the residues 10-50 of the mouse RELM alpha protein.

Reactivity Notes

Mouse

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit FIZZ1/RELM alpha Antibody - BSA Free (NBP2-29355) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-FIZZ1/RELM alpha Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FIZZ1/RELM alpha Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FIZZ1/RELM alpha Antibody - BSA Free [NBP2-29355]

Immunocytochemistry/ Immunofluorescence: FIZZ1/RELM alpha Antibody - BSA Free [NBP2-29355]

Immunocytochemistry/Immunofluorescence: FIZZ1/RELM alpha Antibody [NBP2-29355] - Staining in Mouse BV2 microglia cells, FIZZ1/RELM staining in green and nuclear staining in blue. Image from a verified customer review. Cell treatment with DMSO, DMSO+lipopolysaccharide, SMSO+SP600125 (a JNK inhibitor).
Western Blot: FIZZ1/RELM alpha AntibodyBSA Free [NBP2-29355]

Western Blot: FIZZ1/RELM alpha AntibodyBSA Free [NBP2-29355]

Western Blot: FIZZ1/RELM alpha Antibody [NBP2-29355] - Analysis in mouse BV2 microglia cells. Studing the effects of lysophosphatidic acid (LPA) on microglia polarization state. Dilution at 1:500. Image from a verified customer review.
Western Blot: FIZZ1/RELM alpha AntibodyBSA Free [NBP2-29355]

Western Blot: FIZZ1/RELM alpha AntibodyBSA Free [NBP2-29355]

Western Blot: RELM alpha Antibody [NBP2-29355] - WB detection of secreted Mouse Resistin-Like Molecule alpha (RELM alpha) from media of transfected 293T cells with #NBP2-29355 antibody used at a dilution of 1:500
Western Blot: FIZZ1/RELM alpha AntibodyBSA Free [NBP2-29355]

Western Blot: FIZZ1/RELM alpha AntibodyBSA Free [NBP2-29355]

Western Blot: FIZZ1/RELM alpha Antibody [NBP2-29355] - Functional analysis mouse BV2 cell lysate. Image from a verified customer review.

Applications for FIZZ1/RELM alpha Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500 -1:1000
Application Notes
In Western blot this RELM antibody detects a specific target band between 10-15 kDa position (monomer form) under complete reducing conditions. Larger bands representing dimers or multimers may also be seen if WB run under non-reducing conditions. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. ICC/IF and WB reported in a customer review.

Reviewed Applications

Read 3 reviews rated 4.3 using NBP2-29355 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FIZZ1/RELM alpha

A family of resistin-like molecules (RELMs) has been identified in rodents and humans. Resistin is a hormone produced by fat cells. RELMalpha is a secreted protein that has a restricted tissue distribution with highest levels in adipose tissue. Another family member, RELMbeta, is a secreted protein expressed only in the gastrointestinal tract, particularly the colon, in both mouse and human. RELMbeta gene expression is highest in proliferative epithelial cells and is markedly increased in tumors, suggesting a role in intestinal proliferation. Resistin and the RELMs share a cysteine composition and other signature features. Thus, the RELMs together with resistin comprise a class of tissue-specific signaling molecules.

Long Name

Found in Inflammatory Zone 1/Resistin-like Molecule alpha

Alternate Names

RELM alpha, RETNLA, RETNLB

Entrez Gene IDs

57262 (Mouse)

Gene Symbol

RETNLA

UniProt

Q9EP95

Additional FIZZ1/RELM alpha Products

Product Documents for FIZZ1/RELM alpha Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FIZZ1/RELM alpha Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FIZZ1/RELM alpha Antibody - BSA Free

Customer Reviews for FIZZ1/RELM alpha Antibody - BSA Free (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • FIZZ1/RELM alpha Antibody
    Name: Lisha Joshi
    Application: Western Blot
    Sample Tested: BV2 microglia cells
    Species: Mouse
    Verified Customer | Posted 06/10/2019
    The antibody was used in order to study the effects of lysophosphatidic acid (LPA) on microglia polarization state. It was used in a dilution of 1:500. Using a dilution 1:1000 didn't work so well.
    FIZZ1/RELM alpha Antibody - BSA Free NBP2-29355
  • FIZZ1/RELM alpha Antibody
    Name: Joanna Plastira
    Application: Immunocytochemistry
    Sample Tested: BV2 microglia cells
    Species: Mouse
    Verified Customer | Posted 06/07/2019
    The antibody was used at a concentration 1:100. Having used the antibody for both ICC and WB, I would say that for ICC it worked perfectly. In WB there were some problems with the detection but still a very good antibody to use for both methods.
    FIZZ1/RELM alpha Antibody - BSA Free NBP2-29355
  • FIZZ1/RELM alpha Antibody
    Name: Joanna Plastira
    Application: Western Blot
    Sample Tested: Murine microglia cell lysate and BV2 microglia cell lysate
    Species: Mouse
    Verified Customer | Posted 06/07/2019
    The antibody was used in a concentration 1:500 in order to detect changes in expression levels in BV2 microglia cells.
    FIZZ1/RELM alpha Antibody - BSA Free NBP2-29355

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