FoxO1/FKHR Antibody (23GB850)

Name: FoxO1/FKHR Antibody (23GB850)
Brand: Novus Biologicals
SKU: NBP3-51388
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-51388

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB850

View all FoxO1/FKHR Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human FoxO1/FKHR (Uniprot: Q12778)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FoxO1/FKHR Antibody (23GB850)

Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (23GB850)[NBP3-51388] -

FoxO1/FKHR antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: FOXO1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; FOXO1 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.

Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (23GB850)[NBP3-51388] -

Immunocytochemical staining of C2C12 cells with FoxO1/FKHR antibody (1:1,000). Nuclei were stained blue with DAPI; FoxO1/FKHR was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar, 20 um.

Western Blot: FoxO1/FKHR Antibody (23GB850)[NBP3-51388] -

Western blotting analysis using FoxO1/FKHR antibody. FoxO1/FKHRexpression in wild type (WT) and FoxO1/FKHRshRNA knockdown (KD) HeLa cells with 30 ug of total cell lysates. GAPDH serves as a loading control. The blot was incubated with FoxO1/FKHR antibody(1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (1:50,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for FoxO1/FKHR Antibody (23GB850)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FoxO1/FKHR

FoxO1, also called FKHR (forkhead in rhabdomyosarcoma), is a ubiquitously expressed member of the forkhead box O family of winged helix transcription factors. In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferation. In insulin-responsive tissues, nutrient abundance triggers phosphorylation by AKT that blocks nuclear translocation and activity.

Long Name

Forkhead Box O1

Alternate Names

FKH1, FKHR

Gene Symbol

FOXO1

Additional FoxO1/FKHR Products

Product Documents for FoxO1/FKHR Antibody (23GB850)

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Product Specific Notices for FoxO1/FKHR Antibody (23GB850)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.