FoxP3 Antibody

Novus Biologicals | Catalog # NB600-242

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Control

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FoxP3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence SQRPSRCSNPTPGP corresponding to C-Terminus according to NP_054728.2, NP_001107849.1.

Reactivity Notes

Rat reactivity reported from a verified customer review.

Localization

Nuclear (potential).

Specificity

This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FoxP3 Antibody

Western Blot: FoxP3 Antibody [NB600-242]

Western Blot: FoxP3 Antibody [NB600-242]

Western Blot: FoxP3 Antibody [NB600-242] - (1 ug/mL) staining of Human Muscle (A), (2 ug/mL) MOLT4 (B) and (1 ug/mL) negative control Pancreas (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry: FoxP3 Antibody [NB600-242]

Immunocytochemistry: FoxP3 Antibody [NB600-242]

Immunocytochemistry: FoxP3 Antibody [NB600-242] - (Treg) human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).
Immunohistochemistry: FoxP3 Antibody [NB600-242]

Immunohistochemistry: FoxP3 Antibody [NB600-242]

Immunohistochemistry: FoxP3 Antibody [NB600-242] - (8ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
Flow Cytometry: FoxP3 Antibody [NB600-242]

Flow Cytometry: FoxP3 Antibody [NB600-242]

Flow Cytometry: FoxP3 Antibody [NB600-242] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunofluorescence: FoxP3 Antibody [NB600-242]

Immunofluorescence: FoxP3 Antibody [NB600-242]

Immunofluorescence: FoxP3 Antibody [NB600-242] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing strong localization to nucleoplasm. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).
Control: FoxP3 Antibody [NB600-242] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

Applications for FoxP3 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunohistochemistry-Paraffin

6.0 - 8.0 ug/ml

Peptide ELISA

1:8000

Western Blot

1 - 3 ug/mL
Application Notes
Western blot: Approx 48-50kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2kDa according to NP_054728.2). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate

Reviewed Applications

Read 1 review rated 5 using NB600-242 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FoxP3

FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Entrez Gene IDs

50943 (Human)

Gene Symbol

FOXP3

UniProt

NP_054728.2 (Human)

Additional FoxP3 Products

Product Documents for FoxP3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FoxP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for FoxP3 Antibody (1)

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Customer Images

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  • FoxP3 Antibody
    Name: Luiz Alexandre Magno
    Application: Western Blot
    Sample Tested: PC12 cell whole lysate
    Species: Rat
    Verified Customer | Posted 10/26/2018
    Whole cell lysate of PC12 cells transfected with the FOXP3 plasmid. Band size is around 50 KDa.
    FoxP3 Antibody NB600-242

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FoxP3 Antibody

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  • Q: Does FoxP3 antibodies comes in lyophilized form?

    A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

  • Q: What is the immunogen sequence of this FoxP3 antibody?

    A: A sequence within the C-terminal region (SQRPSRCSNPTPGP) of FoxP3.

  • Q: What the theoretical molecular weight for FoxP3 antibodies?

    A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

  • Q: Does FoxP3 antibodies comes in lyophilized form?

    A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

  • Q: What is the immunogen sequence of this FoxP3 antibody?

    A: A sequence within the C-terminal region (SQRPSRCSNPTPGP) of FoxP3.

  • Q: What the theoretical molecular weight for FoxP3 antibodies?

    A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

  • Q: Does FoxP3 antibodies comes in lyophilized form?

    A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

  • Q: What is the immunogen sequence of this FoxP3 antibody?

    A: A sequence within the C-terminal region (SQRPSRCSNPTPGP) of FoxP3.

  • Q: What the theoretical molecular weight for FoxP3 antibodies?

    A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

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