GAD1/GAD67 Antibody

Novus Biologicals | Catalog # NBP3-48499

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GAD1/GAD67 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAD65/67. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GAD1/GAD67 Antibody

GAD1/GAD67 Antibody

Western Blot: GAD1/GAD67 Antibody [NBP3-48499] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD1/GAD67 antibody (NBP3-48499) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GAD1/GAD67 Antibody

Western Blot: GAD1/GAD67 Antibody [NBP3-48499] -

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD1/GAD67 antibody (NBP3-48499) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GAD1/GAD67 Antibody

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-48499] -

GAD1/GAD67 antibody detects GAD1/GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GAD1/GAD67 stained by GAD1/GAD67 antibody (NBP3-48499) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GAD1/GAD67 Antibody

Immunocytochemistry/ Immunofluorescence: GAD1/GAD67 Antibody [NBP3-48499] -

GAD1/GAD67 antibody detects GAD1/GAD67 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD1/GAD67 protein stained by GAD1/GAD67 antibody (NBP3-48499) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] diluted at 1:500.
Blue: Fluoroshield with DAPI.
GAD1/GAD67 Antibody

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-48499] -

GAD1/GAD67 antibody detects GAD1/GAD67 protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: GAD1/GAD67 protein stained by GAD1/GAD67 antibody (NBP3-48499) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250.
Blue: Fluoroshield with DAPI.
^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GAD1/GAD67 Antibody

Western Blot: GAD1/GAD67 Antibody [NBP3-48499] -

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD1/GAD67 antibody (NBP3-48499) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GAD1/GAD67 Antibody

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-48499] -

GAD1/GAD67 antibody detects GAD1/GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GAD1/GAD67 stained by GAD1/GAD67 antibody (NBP3-48499) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for GAD1/GAD67 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Frozen

Assay Dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAD1/GAD67

GAD65/67 encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Long Name

Glutamate Decarboxylase 1 (Brain, 67kDa)

Alternate Names

GAD67

Gene Symbol

GAD1

Additional GAD1/GAD67 Products

Product Documents for GAD1/GAD67 Antibody

Certificate of Analysis

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Product Specific Notices for GAD1/GAD67 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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