GADD153/CHOP Antibody (9C8) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80745

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Gel Super Shift Assays, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 9C8

Format

Azide and BSA Free
View all GADD153 Primary Antibodies »

Product Specifications

Immunogen

Full length mouse CHOP/GADD153 [Swiss-Prot# P35639]

Epitope

The N-terminal region of CHOP/GADD153.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GADD153/CHOP Antibody (9C8) - Azide and BSA Free

Western Blot: GADD153/CHOP Antibody (9C8)Azide and BSA Free [NBP2-80745]

Western Blot: GADD153/CHOP Antibody (9C8)Azide and BSA Free [NBP2-80745]

Western Blot: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745] - GADD153/CHOP expression in HeLa cells treated with 2.5 ug/mL tunicamycin for 4 hours (Lane 1) and untreated (Lane 2). Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745] - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear
Western Blot: GADD153/CHOP Antibody (9C8)Azide and BSA Free [NBP2-80745]

Western Blot: GADD153/CHOP Antibody (9C8)Azide and BSA Free [NBP2-80745]

Western Blot: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745] - Analysis of CHOP in rat heart tissue lysate. Image courtesy of product review submitted by Lee Hsiao-Wei. Image from the standard format of this antibody.
Western Blot: GADD153/CHOP Antibody (9C8)Azide and BSA Free [NBP2-80745]

Western Blot: GADD153/CHOP Antibody (9C8)Azide and BSA Free [NBP2-80745]

Western Blot: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745] - Analysis of endogenous CHOP/GADD153 from primary human fibroblasts using NB600-1335. Lane 1: Untreated cells, Lane 2: Cells treated with tunicamycin for 10 hours. Image from the standard format of this antibody.
Western Blot: GADD153/CHOP Antibody (9C8)Azide and BSA Free [NBP2-80745]

Western Blot: GADD153/CHOP Antibody (9C8)Azide and BSA Free [NBP2-80745]

Western Blot: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745] - Ethanol feeding increases CHOP expression. Image from verified customer review. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745] - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear
Simple Western: GADD153/CHOP Antibody (9C8)Azide and BSA Free [NBP2-80745]

Simple Western: GADD153/CHOP Antibody (9C8)Azide and BSA Free [NBP2-80745]

Simple Western: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745] - Image shows a specific band for CHOP/GADD153 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.

Applications for GADD153/CHOP Antibody (9C8) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

reported in scientific literature (PMID 30962207)

ELISA

reported in scientific literature (PMID 29915575)

Gel Super Shift Assays

reported in scientific literature

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:10 - 1:500

Simple Western

1:250
Application Notes
In Western blot a band can be seen at approx. 29 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:250. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GADD153

GADD153, also known as CHOP or DDIT3, is as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein functions as a transcriptional inhibitor by forming heterodimers with C/EBP (CCAAT/enhancer-binding protein) and LAP (liver activator protein), and preventing their DNA binding activity. Under normal growth conditions GADD153 is dormant; however it is highly unregulated during cellular stress caused by toxins, metabolic inhibitors and nutrient deprivation. GADD153 is present in the cytosol under nonstressed conditions and then accumulates in the nucleus once it is induced by stress.

Long Name

Growth Arrest and DNA Damage-inducible Protein GADD153

Alternate Names

CEBP zeta, CHOP, CHOP10, DDIT3

Gene Symbol

DDIT3

Additional GADD153 Products

Product Documents for GADD153/CHOP Antibody (9C8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GADD153/CHOP Antibody (9C8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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