Galectin-3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16589

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Rat, Canine

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Galectin-3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Galectin-3. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Galectin-3 Antibody - BSA Free

Western Blot: Galectin-3 Antibody [NBP2-16589]

Western Blot: Galectin-3 Antibody [NBP2-16589]

Western Blot: Galectin-3 Antibody [NBP2-16589] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP2-16589]

Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP2-16589]

Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP2-16589] - HBL435 xenograft, using Galectin3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Galectin-3 Antibody [NBP2-16589]

Western Blot: Galectin-3 Antibody [NBP2-16589]

Western Blot: Galectin-3 Antibody [NBP2-16589] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: LGALS3 transfected 293T lysates 12% SDS PAGE gel, diluted at 1:10000.
Western Blot: Galectin-3 Antibody [NBP2-16589]

Western Blot: Galectin-3 Antibody [NBP2-16589]

Western Blot: Galectin-3 Antibody [NBP2-16589] - Wild-type (WT) and Galectin 3 knownout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Galectin-3 Antibody [NBP2-16589]

Western Blot: Galectin-3 Antibody [NBP2-16589]

Western Blot: Galectin-3 Antibody [NBP2-16589] - Galectin-3 antibody detects Galectin-3 protein by western blot analysis. A. 50 ug rat colon lysate/extract. 12% SDS-PAGE. Galectin-3 antibody dilution: 1:10000.
Sandwich ELISA: Galectin-3 Antibody [NBP2-16589] - Sandwich ELISA detection of recombinant full-length Galectin-3 protein using as capture antibody at concentration of 5 ug/mL and Galectin-3 Antibody as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.
Galectin-3 Antibody

Western Blot: Galectin-3 Antibody [NBP2-16589] -

Western Blot: Galectin-3 Antibody [NBP2-16589] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Galectin-3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody [NBP2-16589] -

Galectin3 antibody detects Galectin3 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: Galectin3 protein stained by Galectin3 antibody (NBP2-16589) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for Galectin-3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Sandwich ELISA

Assay dependent

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Galectin-3

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.

Alternate Names

AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2

Gene Symbol

LGALS3

UniProt

P17931 (Mouse)

Additional Galectin-3 Products

Product Documents for Galectin-3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Galectin-3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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