GATA-3 [p Ser308] Antibody (1E5) - BSA Free

Name: GATA-3 [p Ser308] Antibody (1E5) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-26423
Price: 469 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-26423

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1E5 expressed in HEK293

Format

BSA Free

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Product Specifications

Immunogen

A synthesized peptide derived from Human GATA-3 [p Ser308] [UniProt P23771]

Modification

p Ser 308

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GATA-3 [p Ser308] Antibody (1E5) - BSA Free

Immunohistochemistry: GATA-3 [p Ser308] Antibody (1E5) [NBP3-26423] -

Immunohistochemistry: GATA-3 [p Ser308] Antibody (1E5) [NBP3-26423] - Image of GATA-3 [p Ser308] Antibody (1E5) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot: GATA-3 [p Ser308] Antibody (1E5) [NBP3-26423] -

Western Blot: GATA-3 [p Ser308] Antibody (1E5) [NBP3-26423] - Positive Western Blot detected in Hela whole cell lysate, A549 whole cell lysate.
All lanes: GATA-3 [p Ser308] Antibody at 2.5ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 48 KDa
Observed band size: 48 KDa

Applications for GATA-3 [p Ser308] Antibody (1E5) - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: GATA-3

The GATA3 gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains twoGATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelialcell biology. Defects in th

Alternate Names

GATA3

Gene Symbol

GATA3

Additional GATA-3 Products

Product Documents for GATA-3 [p Ser308] Antibody (1E5) - BSA Free

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Certificate of Analysis

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Product Specific Notices for GATA-3 [p Ser308] Antibody (1E5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.