GATA-4 Antibody

Novus Biologicals | Catalog # NBP2-16613

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (95%), Canine (96%), Porcine (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GATA-4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GATA-4. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (82%), Chicken (85%), Rat (89%)

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GATA-4 Antibody

Western Blot: GATA-4 Antibody [NBP2-16613]

Western Blot: GATA-4 Antibody [NBP2-16613]

Western Blot: GATA-4 Antibody [NBP2-16613] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GATA4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: GATA-4 Antibody [NBP2-16613]

Immunocytochemistry/ Immunofluorescence: GATA-4 Antibody [NBP2-16613]

Immunocytochemistry/Immunofluorescence: GATA4 Antibody [NBP2-16613] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-16613]

Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-16613]

Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-16613] - Mouse duodenum. GATA4 stained by GATA4 antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-16613]

Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-16613]

Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-16613] - Mouse E13.5 embryo.Green: GATA4 stained by GATA4 antibody diluted at 1:250.Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GATA-4 Antibody

Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-16613] -

GATA4 antibody detects GATA4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
GATA4 stained by GATA4 antibody (NBP2-16613) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GATA-4 Antibody

Immunocytochemistry/ Immunofluorescence: GATA-4 Antibody [NBP2-16613] -

GATA4 antibody detects GATA4 protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GATA4 stained by GATA4 antibody (NBP2-16613) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
GATA-4 Antibody

Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-16613] -

GATA4 antibody detects GATA4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
GATA4 stained by GATA4 antibody (NBP2-16613) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for GATA-4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GATA-4

This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects. [provided by RefSeq]

Alternate Names

GATA4

Gene Symbol

GATA4

UniProt

P43694

Additional GATA-4 Products

Product Documents for GATA-4 Antibody

Certificate of Analysis

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Product Specific Notices for GATA-4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GATA-4 Antibody

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Protocols

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