GBX2 Antibody

Novus Biologicals | Catalog # NB100-55393

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GBX2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QGKDESKVEDDPK corresponding to internal region according to NP_001476.2.

Epitope

QGKDESKVEDDPK

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23144817).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GBX2 Antibody

Immunocytochemistry/ Immunofluorescence: GBX2 Antibody [NB100-55393]

Immunocytochemistry/ Immunofluorescence: GBX2 Antibody [NB100-55393]

Immunocytochemistry/Immunofluorescence: GBX2 Antibody [NB100-55393] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393]

Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393]

Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393] - Staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunocytochemistry/ Immunofluorescence: GBX2 Antibody [NB100-55393]

Immunocytochemistry/ Immunofluorescence: GBX2 Antibody [NB100-55393]

Immunocytochemistry/Immunofluorescence: GBX2 Antibody [NB100-55393] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393]

Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393]

Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393] - Staining of paraffin embedded Human Placenta at dilution of 5ug/ml. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393]

Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393]

Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393] - Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.

Applications for GBX2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:18000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
IHC-P: Human placenta shows nuclear staining of selected trophoblasts.
IHC: In paraffin embedded Human Placenta shows nuclear staining of selected trophoblasts. WB reactivity reported in (PMID: 23144817).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GBX2

GBX2 (Gastrulation brain homeobox 2) is a homeobox protein which is involved in enbryonic cell pluripotency and differentiation. GBX2 is expressed in all three embryonic germ layers of the late gastrula/early neurula. Gbx2 is overexpressed in a panel of human prostatic cancer cell lines (TSU-pr1, PC3, DU145, LNCaP).

Long Name

Gastrulation Brain Homeobox 2

Alternate Names

Gastrulation and brain-specific homeobox protein 2, gastrulation brain homeo box 2, gastrulation brain homeobox 2, homeobox protein GBX-2

Entrez Gene IDs

2637 (Human); 14472 (Mouse); 114500 (Rat)

Gene Symbol

GBX2

UniProt

NP_001476.2 (Human)

Additional GBX2 Products

Product Documents for GBX2 Antibody

Certificate of Analysis

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Product Specific Notices for GBX2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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