GDF-8/Myostatin Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DGDF80
Control Products Available
Ancillary Products Available
Multi-species GDF-8/Myostatin ELISA Standard Curve
2 Images
Product Details
Procedure
Citations (28)
FAQs
Supplemental Products
Reviews (3)

GDF-8/Myostatin Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Tissue Homogenates (20 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
5.32 pg/mL
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human, mouse, rat, canine, porcine, and equine GDF-8.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine GDF-8/Myostatin Immunoassay is a 4.5 hour solid phase ELISA designed to measure GDF-8 levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant GDF-8 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant GDF-8 accurately. Results obtained using natural GDF-8 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural GDF-8.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 171 583 1147 146 491 1058
Standard Deviation 9.2 14.8 20.9 8.8 17.7 32.3
CV% 5.4 2.5 1.8 6 3.6 3.1

Recovery

The recovery of GDF-8 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 110 100-118
Serum and Plasma (n=12) 91 80-117
Tissue Homogenates (n=4) 100 90-120

Linearity

To assess the linearity of the assay, samples containing high concentrations of GDF-8 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Multi-species GDF-8/Myostatin ELISA Linearity

Data Examples

Multi-species GDF-8/Myostatin ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GDF-8/Myostatin

Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.

Long Name:
Growth Differentiation Factor 8
Entrez Gene IDs:
2660 (Human); 17700 (Mouse)
Alternate Names:
GDF8; GDF-8; GDF8growth differentiation factor 8; growth/differentiation factor 8; MSLHP; MSTN; Myostatin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for GDF-8/Myostatin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Iron Status in Elderly Women Impacts Myostatin, Adiponectin and Osteocalcin Levels Induced by Nordic Walking Training
    Authors: J Kortas, E Ziemann, D Juszczak, K Micielska, M Koz?owska, K Prusik, K Prusik, J Antosiewic
    Nutrients, 2020;12(4):.
    Species: Human
    Sample Types: Serum
  2. miR-27a regulates vascular remodeling by targeting endothelial cells' apoptosis and interaction with vascular smooth muscle cells in aortic dissection
    Authors: Y Sun, Y Xiao, H Sun, Z Zhao, J Zhu, L Zhang, J Dong, T Han, Q Jing, J Zhou, Z Jing
    Theranostics, 2019;9(25):7961-7975.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Down-regulation of the mitochondrial i-AAA protease Yme1L induces muscle atrophy via FoxO3a and myostatin activation
    Authors: YJ Lee, GH Kim, SI Park, JH Lim
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Mouse
    Sample Types: Serum
  4. Significance of serum Myostatin in hemodialysis patients
    Authors: P Esposito, Y Battaglia, E La Porta, MA Grignano, E Caramella, A Avella, S Peressini, N Sessa, R Albertini, G Di Natali, C Lisi, M Gregorini, T Rampino
    BMC Nephrol, 2019;20(1):462.
    Species: Human
    Sample Types: Serum
  5. Effects of dapagliflozin on the serum levels of fibroblast growth factor 21 and myokines and muscle mass in Japanese patients with type 2 diabetes: a randomized, controlled trial
    Authors: H Yamakage, M Tanaka, T Inoue, S Odori, T Kusakabe, N Satoh-Asah
    J Diabetes Investig, 2019;0(0):.
    Species: Human
    Sample Types: Serum
  6. Sex differences in body composition but not neuromuscular function following long-term, doxycycline-induced reduction in circulating levels of myostatin in mice
    Authors: D Tavoian, WD Arnold, SC Mort, S de Lacalle
    PLoS ONE, 2019;14(11):e0225283.
    Species: Mouse
    Sample Types: Serum
  7. Randomised trial on clinical performances and biocompatibility of four high-flux hemodialyzers in two mode treatments: hemodialysis vs post dilution hemodiafiltration
    Authors: M Morena, C Creput, M Bouzernidj, A Rodriguez, L Chalabi, B Seigneuric, C Lauret, AS Bargnoux, AM Dupuy, JP Cristol
    Sci Rep, 2019;9(1):18265.
    Species: Human
    Sample Types: Serum
  8. Supraphysiologic Administration of GDF11 Induces Cachexia in Part by Upregulating GDF15
    Authors: JE Jones, SM Cadena, C Gong, X Wang, Z Chen, SX Wang, C Vickers, H Chen, E Lach-Trifi, JR Hadcock, DJ Glass
    Cell Rep, 2018;22(6):1522-1530.
    Species: Mouse
    Sample Types: Plasma
  9. Interconnected Microphysiological Systems for Quantitative Biology and Pharmacology Studies
    Authors: CD Edington, WLK Chen, E Geishecker, T Kassis, LR Soenksen, BM Bhushan, D Freake, J Kirschner, C Maass, N Tsamandour, J Valdez, CD Cook, T Parent, S Snyder, J Yu, E Suter, M Shockley, J Velazquez, JJ Velazquez, L Stockdale, JP Papps, I Lee, N Vann, M Gamboa, ME LaBarge, Z Zhong, X Wang, LA Boyer, DA Lauffenbur, RL Carrier, C Communal, SR Tannenbaum, CL Stokes, DJ Hughes, G Rohatgi, DL Trumper, M Cirit, LG Griffith
    Sci Rep, 2018;8(1):4530.
  10. Plasma Levels of Myonectin But Not Myostatin or Fibroblast-Derived Growth Factor 21 Are Associated with Insulin Resistance in Adult Humans without Diabetes Mellitus
    Authors: FJK Toloza, JO Mantilla-R, MC Pérez-Mato, ML Ricardo-Si, MC Morales-Al, JA Pinzón-Cor, M Pérez-Mayo, ML Arévalo-Ga, G Tolosa-Gon, CO Mendivil
    Front Endocrinol (Lausanne), 2018;9(0):5.
    Species: Human
    Sample Types: Plasma
  11. Myokine Response to High-Intensity Interval vs. Resistance Exercise: An Individual Approach
    Authors: Z He, Y Tian, PL Valenzuela, C Huang, J Zhao, P Hong, Z He, S Yin, A Lucia
    Front Physiol, 2018;9(0):1735.
    Species: Human
    Sample Types: Serum
  12. Differential response of adipose tissue gene and protein expressions to 4- and 8-week administration of ?-guanidinopropionic acid in mice
    Authors: H Kato, S Masuda, T Ohira, L Ohira, H Takakura, Y Ohira, T Izawa
    Physiol Rep, 2018;6(5):.
    Species: Mouse
    Sample Types: Serum
  13. A 2-Week Specific Volleyball Training Supported by the Whole Body Cryostimulation Protocol Induced an Increase of Growth Factors and Counteracted Deterioration of Physical Performance
    Authors: J Jaworska, K Micielska, M Koz?owska, K Wnorowski, J Skrobecki, L Radzimi?sk, A Babi?ska, E Rodziewicz, G Lombardi, E Ziemann
    Front Physiol, 2018;9(0):1711.
    Species: Human
    Sample Types: Serum
  14. Activin subfamily peptides predict chronological age in humans
    Authors: LV Barrios-Si, M Parnell, ZB Shinwari, GA Chaudhary, T Xenofontos, A van Bekhov, S McArthur, BT Elliott
    Physiol Rep, 2018;6(17):e13823.
    Species: Human
    Sample Types: Plasma
  15. Relative abundance of mature myostatin rather than total myostatin is negatively associated with bone mineral density in Chinese
    Authors: LF Wu, DC Zhu, BH Wang, YH Lu, P He, YH Zhang, HQ Gao, XW Zhu, W Xia, H Zhu, XB Mo, X Lu, L Zhang, YH Zhang, FY Deng, SF Lei
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Human
    Sample Types: Plasma
  16. Lifelong exercise, but not short-term high-intensity interval training, increases GDF11, a marker of successful aging: a�preliminary investigation
    Authors: BT Elliott, P Herbert, N Sculthorpe, FM Grace, D Stratton, LD Hayes
    Physiol Rep, 2017;5(13):.
    Species: Human
    Sample Types: Serum
  17. Alterations in Adiposity and Glucose Homeostasis in Adult Gasp-1 Overexpressing Mice
    Authors: L Périè, A Parenté, F Baraige, L Magnol, V Blanquet
    Cell. Physiol. Biochem., 2017;44(5):1896-1911.
    Species: Mouse
    Sample Types: Plasma
  18. Activin A more prominently regulates muscle mass in primates than does GDF8
    Authors: E Latres, J Mastaitis, W Fury, L Miloscio, J Trejos, J Pangilinan, H Okamoto, K Cavino, E Na, A Papatheodo, T Willer, Y Bai, J Hae Kim, A Rafique, S Jaspers, T Stitt, AJ Murphy, GD Yancopoulo, J Gromada
    Nat Commun, 2017;8(0):15153.
    Species: Human
    Sample Types: Serum
  19. Myostatin and adipokines: The role of the metabolically unhealthy obese phenotype in muscle function and aerobic capacity in young adults
    Authors: LP Carvalho, RP Basso-Vane, L Di Thommaz, RG Mendes, MC Oliveira-J, RP Vieira, JC Bonjorno-J, CR Oliveira, R Luporini, A Borghi-Sil
    Cytokine, 2017;0(0):.
    Species: Human
    Sample Types: Plasma
  20. Serum Biomarkers of Myocardial Remodeling and Coronary Dysfunction in Early Stages of Hypertrophic Cardiomyopathy in the Young
    Authors: E Fernlund, T Gyllenhamm, R Jablonowsk, M Carlsson, A Larsson, J Ärnlöv, P Liuba
    Pediatr Cardiol, 2017;0(0):.
    Species: Human
    Sample Types: Serum
  21. Serum GDF-8 levels change dynamically during controlled ovarian hyperstimulation in patients undergoing IVF/ICSI-ET
    Authors: Lanlan Fang
    Sci Rep, 2016;6(0):28036.
    Species: Human
    Sample Types: Serum
  22. Decreasing maternal myostatin programs adult offspring bone strength in a mouse model of osteogenesis imperfecta
    Proc. Natl. Acad. Sci. U.S.A., 2016;0(0):.
    Species: Mouse
    Sample Types: Serum
  23. Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets.
    Authors: Rao S, Fujimura T, Matsunari H, Sakuma T, Nakano K, Watanabe M, Asano Y, Kitagawa E, Yamamoto T, Nagashima H
    Mol Reprod Dev, 2016;83(1):61-70.
    Species: Porcine
    Sample Types: Tissue Homogenates
  24. Supplementation of Magnolol Attenuates Skeletal Muscle Atrophy in Bladder Cancer-Bearing Mice Undergoing Chemotherapy via Suppression of FoxO3 Activation and Induction of IGF-1.
    Authors: Chen M, Chen Y, Lee C, Hung C, Chou T
    PLoS ONE, 2015;10(11):e0143594.
    Species: Mouse
    Sample Types: Tissue Homogenates
  25. Relationship between Blood Myostatin Levels and Kidney Function:Shimane CoHRE Study.
    Authors: Yano S, Nagai A, Isomura M, Yamasaki M, Kijima T, Takeda M, Hamano T, Nabika T
    PLoS ONE, 2015;10(10):e0141035.
    Species: Human
    Sample Types: Plasma
  26. Preliminary investigation into a potential role for myostatin and its receptor (ActRIIB) in lean and obese horses and ponies.
    Authors: Morrison P, Bing C, Harris P, Maltin C, Grove-White D, Argo C
    PLoS ONE, 2014;9(11):e112621.
    Species: Equine
    Sample Types: Serum
  27. Myostatin regulates energy homeostasis in the heart and prevents heart failure.
    Authors: Biesemann N, Mendler L, Wietelmann A, Hermann S, Schafers M, Kruger M, Boettger T, Borchardt T, Braun T
    Circ Res, 2014;115(2):296-310.
    Species: Mouse
    Sample Types: Serum
  28. Activin subfamily peptides predict chronological age in humans.
    Authors: Barrios-Silva L, Parnell M, Shinwari Z, Chaudhary G, Xenofontos T, van Bekhoven A, McArthur S, Elliott B
    Physiol Rep, 0;6(17):e13823.
    Species: Human
    Sample Types: Plasma

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Reviews for GDF-8/Myostatin Quantikine ELISA Kit

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GDF-8/Myostatin Quantikine ELISA Kit
By Anonymous on 03/14/2019
Application: Sample Tested: Serum and Plasma

GDF-8/Myostatin Quantikine ELISA Kit
By Anonymous on 02/20/2018
Application: Sample Tested: Serum,Mouse Serum,Human Serum

Great kit overall. Standard curve is very reproducible (R2>0.999), and good above suggested limits. Easy to follow protocol. Great, detailed suggestion on sample dilutions and expected ranges for different samples.


GDF-8/Myostatin Quantikine ELISA Kit
By Anonymous on 01/31/2018
Application: Sample Tested: Heparin Plasma,EDTA Plasma,Serum

Ran assay successfully with human plasma (Heparin and EDTA) and human serum (SST) samples, with and without binding protein separation step. High linearity of standard curve (example shown produced by undergrad researchers) when run in triplicate.