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GDF-8/Myostatin Quantikine ELISA Kit

R&D Systems | Catalog # DGDF80

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Tissue Homogenates (20 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)

Sensitivity

5.32 pg/mL

Assay Range

31.3-2000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for GDF-8/Myostatin Quantikine ELISA Kit

The Quantikine GDF-8/Myostatin Immunoassay is a 4.5 hour solid phase ELISA designed to measure GDF-8 levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant GDF-8 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant GDF-8 accurately. Results obtained using natural GDF-8 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural GDF-8.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Multi-Species

Specificity

Natural and recombinant human, mouse, rat, canine, porcine, and equine GDF-8.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 171 583 1147 146 491 1058
Standard Deviation 9.20 14.8 20.9 8.80 17.7 32.3
CV% 5.4 2.5 1.8 6.0 3.6 3.1

Recovery for GDF-8/Myostatin Quantikine ELISA Kit

The recovery of GDF-8 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 110 100-118
Serum and Plasma (n=12) 91 80-117
Tissue Homogenates (n=4) 100 90-120

Linearity

To assess the linearity of the assay, samples containing high concentrations of GDF-8 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

Multi-species GDF-8/Myostatin ELISA Linearity

Scientific Data Images for GDF-8/Myostatin Quantikine ELISA Kit

Multi-species GDF-8/Myostatin ELISA Standard Curve

Multi-species GDF-8/Myostatin ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GDF-8/Myostatin

GDF-8/Myostatin is a secreted protein that is expressed in developing and adult skeletal muscle. It controls myoblast proliferation and is a potent negative regulator of skeletal muscle mass. It is upregulated in mechanically-stressed cardiomyocytes and induces the skeletal muscle wasting which is common in heart failure. The GDF-8 propeptide is cleaved from GDF-8 but remains associated and functions as an inhibitor of mature GDF-8. GDF-8 activity can also be inhibited through its association with Follistatin, FLRG, Decorin, or GASP-1. Active GDF-8 signals through a receptor complex containing Activin RIIB and either Activin RIB/ALK-4 or TGF-beta RI/ALK-5.

Long Name

Growth Differentiation Factor 8

Alternate Names

GDF8, MSLHP, MSTN, Myostatin

Entrez Gene IDs

2660 (Human); 17700 (Mouse)

Gene Symbol

MSTN

Additional GDF-8/Myostatin Products

Product Documents for GDF-8/Myostatin Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for GDF-8/Myostatin Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for GDF-8/Myostatin Quantikine ELISA Kit

Customer Reviews for GDF-8/Myostatin Quantikine ELISA Kit (4)

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4 Customer Ratings
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  • GDF-8/Myostatin Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 11/30/2020
    GDF-8/Myostatin Quantikine ELISA Kit DGDF80
  • GDF-8/Myostatin Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 03/14/2019
    GDF-8/Myostatin Quantikine ELISA Kit DGDF80
  • GDF-8/Myostatin Quantikine ELISA Kit
    Name: Travis Muff
    Sample Tested: Serum, mouse serum and Human serum
    Verified Customer | Posted 02/20/2018
    Great kit overall. Standard curve is very reproducible (R2>0.999), and good above suggested limits. Easy to follow protocol. Great, detailed suggestion on sample dilutions and expected ranges for different samples.
    GDF-8/Myostatin Quantikine ELISA Kit DGDF80
  • GDF-8/Myostatin Quantikine ELISA Kit
    Name: Bradley Elliott
    Sample Tested: Heparin Plasma, EDTA Plasma and Serum
    Verified Customer | Posted 01/31/2018
    Ran assay successfully with human plasma (Heparin and EDTA) and human serum (SST) samples, with and without binding protein separation step. High linearity of standard curve (example shown produced by undergrad researchers) when run in triplicate.
    GDF-8/Myostatin Quantikine ELISA Kit DGDF80

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Protocols

View specific protocols for GDF-8/Myostatin Quantikine ELISA Kit (DGDF80):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for GDF-8/Myostatin Quantikine ELISA Kit

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  • Q: What form of GDF-8/Myostatin does this kit recognize?

    A: This ELISA is specific for the mature active protein. 

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