GDF-8/Myostatin Quantikine ELISA Kit
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Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
GDF-8/Myostatin Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Tissue Homogenates (20 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
5.32 pg/mL
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human, mouse, rat, canine, porcine, and equine GDF-8.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine GDF-8/Myostatin Immunoassay is a 4.5 hour solid phase ELISA designed to measure GDF-8 levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant GDF-8 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant GDF-8 accurately. Results obtained using natural GDF-8 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural GDF-8.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in twenty separate
assays to assess inter-assay precision. Assays
were performed by at least three technicians using two lots of kit components
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 171 | 583 | 1147 | 146 | 491 | 1058 |
| Standard Deviation | 9.2 | 14.8 | 20.9 | 8.8 | 17.7 | 32.3 |
| CV% | 5.4 | 2.5 | 1.8 | 6 | 3.6 | 3.1 |
Recovery
The recovery of GDF-8 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 110 | 100-118 |
| Serum and Plasma (n=12) | 91 | 80-117 |
| Tissue Homogenates (n=4) | 100 | 90-120 |
Linearity
To assess the linearity of the assay, samples containing high
concentrations of GDF-8 were serially diluted with Calibrator Diluent to
produce samples with values within the dynamic range of the assay.
Samples were diluted prior to assay.
Data Examples
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: GDF-8/Myostatin
Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.
Long Name:
Growth Differentiation Factor 8
Entrez Gene IDs:
2660 (Human); 17700 (Mouse)
Alternate Names:
GDF8; GDF-8; GDF8growth differentiation factor 8; growth/differentiation factor 8; MSLHP; MSTN; Myostatin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Citations for GDF-8/Myostatin Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Significance of serum Myostatin in hemodialysis patients
Authors: P Esposito, Y Battaglia, E La Porta, MA Grignano, E Caramella, A Avella, S Peressini, N Sessa, R Albertini, G Di Natali, C Lisi, M Gregorini, T Rampino
BMC Nephrol, 2019;20(1):462.
Species: Human
Sample Types: Serum
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Effects of dapagliflozin on the serum levels of fibroblast growth factor 21 and myokines and muscle mass in Japanese patients with type 2 diabetes: a randomized, controlled trial
Authors: H Yamakage, M Tanaka, T Inoue, S Odori, T Kusakabe, N Satoh-Asah
J Diabetes Investig, 2019;0(0):.
Species: Human
Sample Types: Serum
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Sex differences in body composition but not neuromuscular function following long-term, doxycycline-induced reduction in circulating levels of myostatin in mice
Authors: D Tavoian, WD Arnold, SC Mort, S de Lacalle
PLoS ONE, 2019;14(11):e0225283.
Species: Mouse
Sample Types: Serum
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Randomised trial on clinical performances and biocompatibility of four high-flux hemodialyzers in two mode treatments: hemodialysis vs post dilution hemodiafiltration
Authors: M Morena, C Creput, M Bouzernidj, A Rodriguez, L Chalabi, B Seigneuric, C Lauret, AS Bargnoux, AM Dupuy, JP Cristol
Sci Rep, 2019;9(1):18265.
Species: Human
Sample Types: Serum
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Differential response of adipose tissue gene and protein expressions to 4- and 8-week administration of ?-guanidinopropionic acid in mice
Authors: H Kato, S Masuda, T Ohira, L Ohira, H Takakura, Y Ohira, T Izawa
Physiol Rep, 2018;6(5):.
Species: Mouse
Sample Types: Serum
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Interconnected Microphysiological Systems for Quantitative Biology and Pharmacology Studies
Authors: CD Edington, WLK Chen, E Geishecker, T Kassis, LR Soenksen, BM Bhushan, D Freake, J Kirschner, C Maass, N Tsamandour, J Valdez, CD Cook, T Parent, S Snyder, J Yu, E Suter, M Shockley, J Velazquez, JJ Velazquez, L Stockdale, JP Papps, I Lee, N Vann, M Gamboa, ME LaBarge, Z Zhong, X Wang, LA Boyer, DA Lauffenbur, RL Carrier, C Communal, SR Tannenbaum, CL Stokes, DJ Hughes, G Rohatgi, DL Trumper, M Cirit, LG Griffith
Sci Rep, 2018;8(1):4530.
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Plasma Levels of Myonectin But Not Myostatin or Fibroblast-Derived Growth Factor 21 Are Associated with Insulin Resistance in Adult Humans without Diabetes Mellitus
Authors: FJK Toloza, JO Mantilla-R, MC Pérez-Mato, ML Ricardo-Si, MC Morales-Al, JA Pinzón-Cor, M Pérez-Mayo, ML Arévalo-Ga, G Tolosa-Gon, CO Mendivil
Front Endocrinol (Lausanne), 2018;9(0):5.
Species: Human
Sample Types: Plasma
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Myokine Response to High-Intensity Interval vs. Resistance Exercise: An Individual Approach
Authors: Z He, Y Tian, PL Valenzuela, C Huang, J Zhao, P Hong, Z He, S Yin, A Lucia
Front Physiol, 2018;9(0):1735.
Species: Human
Sample Types: Serum
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A 2-Week Specific Volleyball Training Supported by the Whole Body Cryostimulation Protocol Induced an Increase of Growth Factors and Counteracted Deterioration of Physical Performance
Authors: J Jaworska, K Micielska, M Koz?owska, K Wnorowski, J Skrobecki, L Radzimi?sk, A Babi?ska, E Rodziewicz, G Lombardi, E Ziemann
Front Physiol, 2018;9(0):1711.
Species: Human
Sample Types: Serum
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Activin subfamily peptides predict chronological age in humans
Authors: LV Barrios-Si, M Parnell, ZB Shinwari, GA Chaudhary, T Xenofontos, A van Bekhov, S McArthur, BT Elliott
Physiol Rep, 2018;6(17):e13823.
Species: Human
Sample Types: Plasma
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Supraphysiologic Administration of GDF11 Induces Cachexia in Part by Upregulating GDF15
Authors: JE Jones, SM Cadena, C Gong, X Wang, Z Chen, SX Wang, C Vickers, H Chen, E Lach-Trifi, JR Hadcock, DJ Glass
Cell Rep, 2018;22(6):1522-1530.
Species: Mouse
Sample Types: Plasma
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Relative abundance of mature myostatin rather than total myostatin is negatively associated with bone mineral density in Chinese
Authors: LF Wu, DC Zhu, BH Wang, YH Lu, P He, YH Zhang, HQ Gao, XW Zhu, W Xia, H Zhu, XB Mo, X Lu, L Zhang, YH Zhang, FY Deng, SF Lei
J. Cell. Mol. Med., 2017;0(0):.
Species: Human
Sample Types: Plasma
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Lifelong exercise, but not short-term high-intensity interval training, increases GDF11, a marker of successful aging: a�preliminary investigation
Authors: BT Elliott, P Herbert, N Sculthorpe, FM Grace, D Stratton, LD Hayes
Physiol Rep, 2017;5(13):.
Species: Human
Sample Types: Serum
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Alterations in Adiposity and Glucose Homeostasis in Adult Gasp-1 Overexpressing Mice
Authors: L Périè, A Parenté, F Baraige, L Magnol, V Blanquet
Cell. Physiol. Biochem., 2017;44(5):1896-1911.
Species: Mouse
Sample Types: Plasma
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Activin A more prominently regulates muscle mass in primates than does GDF8
Authors: E Latres, J Mastaitis, W Fury, L Miloscio, J Trejos, J Pangilinan, H Okamoto, K Cavino, E Na, A Papatheodo, T Willer, Y Bai, J Hae Kim, A Rafique, S Jaspers, T Stitt, AJ Murphy, GD Yancopoulo, J Gromada
Nat Commun, 2017;8(0):15153.
Species: Human
Sample Types: Serum
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Myostatin and adipokines: The role of the metabolically unhealthy obese phenotype in muscle function and aerobic capacity in young adults
Authors: LP Carvalho, RP Basso-Vane, L Di Thommaz, RG Mendes, MC Oliveira-J, RP Vieira, JC Bonjorno-J, CR Oliveira, R Luporini, A Borghi-Sil
Cytokine, 2017;0(0):.
Species: Human
Sample Types: Plasma
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Serum Biomarkers of Myocardial Remodeling and Coronary Dysfunction in Early Stages of Hypertrophic Cardiomyopathy in the Young
Authors: E Fernlund, T Gyllenhamm, R Jablonowsk, M Carlsson, A Larsson, J Ärnlöv, P Liuba
Pediatr Cardiol, 2017;0(0):.
Species: Human
Sample Types: Serum
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Serum GDF-8 levels change dynamically during controlled ovarian hyperstimulation in patients undergoing IVF/ICSI-ET
Authors: Lanlan Fang
Sci Rep, 2016;6(0):28036.
Species: Human
Sample Types: Serum
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Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets.
Authors: Rao S, Fujimura T, Matsunari H, Sakuma T, Nakano K, Watanabe M, Asano Y, Kitagawa E, Yamamoto T, Nagashima H
Mol Reprod Dev, 2016;83(1):61-70.
Species: Porcine
Sample Types: Tissue Homogenates
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Decreasing maternal myostatin programs adult offspring bone strength in a mouse model of osteogenesis imperfecta
Proc. Natl. Acad. Sci. U.S.A., 2016;0(0):.
Species: Mouse
Sample Types: Serum
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Supplementation of Magnolol Attenuates Skeletal Muscle Atrophy in Bladder Cancer-Bearing Mice Undergoing Chemotherapy via Suppression of FoxO3 Activation and Induction of IGF-1.
Authors: Chen M, Chen Y, Lee C, Hung C, Chou T
PLoS ONE, 2015;10(11):e0143594.
Species: Mouse
Sample Types: Tissue Homogenates
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Relationship between Blood Myostatin Levels and Kidney Function:Shimane CoHRE Study.
Authors: Yano S, Nagai A, Isomura M, Yamasaki M, Kijima T, Takeda M, Hamano T, Nabika T
PLoS ONE, 2015;10(10):e0141035.
Species: Human
Sample Types: Plasma
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Preliminary investigation into a potential role for myostatin and its receptor (ActRIIB) in lean and obese horses and ponies.
Authors: Morrison P, Bing C, Harris P, Maltin C, Grove-White D, Argo C
PLoS ONE, 2014;9(11):e112621.
Species: Equine
Sample Types: Serum
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Myostatin regulates energy homeostasis in the heart and prevents heart failure.
Authors: Biesemann N, Mendler L, Wietelmann A, Hermann S, Schafers M, Kruger M, Boettger T, Borchardt T, Braun T
Circ Res, 2014;115(2):296-310.
Species: Mouse
Sample Types: Serum
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Activin subfamily peptides predict chronological age in humans.
Authors: Barrios-Silva L, Parnell M, Shinwari Z, Chaudhary G, Xenofontos T, van Bekhoven A, McArthur S, Elliott B
Physiol Rep, 0;6(17):e13823.
Species: Human
Sample Types: Plasma
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Reviews for GDF-8/Myostatin Quantikine ELISA Kit
Average Rating: 5 (Based on 3 Reviews)
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GDF-8/Myostatin Quantikine ELISA Kit
By Anonymous
on 03/14/2019
Application:
Sample Tested: Serum and Plasma
GDF-8/Myostatin Quantikine ELISA Kit
By Anonymous
on 02/20/2018
Application:
Sample Tested: Serum,Mouse Serum,Human Serum
Great kit overall. Standard curve is very reproducible (R2>0.999), and good above suggested limits. Easy to follow protocol. Great, detailed suggestion on sample dilutions and expected ranges for different samples.
GDF-8/Myostatin Quantikine ELISA Kit
By Anonymous
on 01/31/2018
Application:
Sample Tested: Heparin Plasma,EDTA Plasma,Serum
Ran assay successfully with human plasma (Heparin and EDTA) and human serum (SST) samples, with and without binding protein separation step. High linearity of standard curve (example shown produced by undergrad researchers) when run in triplicate.
