GDF-8/Myostatin Quantikine ELISA Kit

  (16 citations)
(2 Reviews)
    
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Assay Procedure
Citations (16)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Tissue Homogenates (20 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    5.32 pg/mL
  • Assay Range
    31.3 - 2,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human, mouse, rat, canine, porcine, and equine GDF-8.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Ancillary Reagent Kit Available
DY010 , Sample Activation Kit 1 - Please Inquire
Control Available
QC98 , Quantikine Immunoassay Control Set 794 Human GDF-8/Myostatin - Please Inquire
Product Summary
The Quantikine GDF-8/Myostatin Immunoassay is a 4.5 hour solid phase ELISA designed to measure GDF-8 levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant GDF-8 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant GDF-8 accurately. Results obtained using natural GDF-8 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural GDF-8.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 171 583 1147 146 491 1058
Standard Deviation 9.2 14.8 20.9 8.8 17.7 32.3
CV% 5.4 2.5 1.8 6 3.6 3.1

Recovery

The recovery of GDF-8 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 110 100-118
Serum and Plasma (n=12) 91 80-117
Tissue Homogenates (n=4) 100 90-120
Linearity
To assess the linearity of the assay, samples containing high concentrations of GDF-8 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
 GDF-8/Myostatin [HRP]
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: GDF-8/Myostatin
Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.
  • Long Name:
    Growth Differentiation Factor 8
  • Entrez Gene IDs:
    2660 (Human); 17700 (Mouse)
  • Alternate Names:
    GDF8; GDF-8; GDF8growth differentiation factor 8; growth/differentiation factor 8; MSTN; Myostatin
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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Species
Sample Type
  1. Supraphysiologic Administration of GDF11 Induces Cachexia in Part by Upregulating GDF15
    Authors: JE Jones, SM Cadena, C Gong, X Wang, Z Chen, SX Wang, C Vickers, H Chen, E Lach-Trifi, JR Hadcock, DJ Glass
    Cell Rep, 2018;22(6):1522-1530.
    Species: Mouse
    Sample Type: Plasma
  2. Differential response of adipose tissue gene and protein expressions to 4- and 8-week administration of ?-guanidinopropionic acid in mice
    Authors: H Kato, S Masuda, T Ohira, L Ohira, H Takakura, Y Ohira, T Izawa
    Physiol Rep, 2018;6(5):.
    Species: Mouse
    Sample Type: Serum
  3. Interconnected Microphysiological Systems for Quantitative Biology and Pharmacology Studies
    Authors: CD Edington, WLK Chen, E Geishecker, T Kassis, LR Soenksen, BM Bhushan, D Freake, J Kirschner, C Maass, N Tsamandour, J Valdez, CD Cook, T Parent, S Snyder, J Yu, E Suter, M Shockley, J Velazquez, JJ Velazquez, L Stockdale, JP Papps, I Lee, N Vann, M Gamboa, ME LaBarge, Z Zhong, X Wang, LA Boyer, DA Lauffenbur, RL Carrier, C Communal, SR Tannenbaum, CL Stokes, DJ Hughes, G Rohatgi, DL Trumper, M Cirit, LG Griffith
    Sci Rep, 2018;8(1):4530.
  4. Lifelong exercise, but not short-term high-intensity interval training, increases GDF11, a marker of successful aging: a�preliminary investigation
    Authors: BT Elliott, P Herbert, N Sculthorpe, FM Grace, D Stratton, LD Hayes
    Physiol Rep, 2017;5(13):.
    Species: Human
    Sample Type: Serum
  5. Activin A more prominently regulates muscle mass in primates than does GDF8
    Authors: E Latres, J Mastaitis, W Fury, L Miloscio, J Trejos, J Pangilinan, H Okamoto, K Cavino, E Na, A Papatheodo, T Willer, Y Bai, J Hae Kim, A Rafique, S Jaspers, T Stitt, AJ Murphy, GD Yancopoulo, J Gromada
    Nat Commun, 2017;8(0):15153.
    Species: Human
    Sample Type: Serum
  6. Alterations in Adiposity and Glucose Homeostasis in Adult Gasp-1 Overexpressing Mice
    Authors: L Périè, A Parenté, F Baraige, L Magnol, V Blanquet
    Cell. Physiol. Biochem., 2017;44(5):1896-1911.
    Species: Mouse
    Sample Type: Plasma
  7. Relative abundance of mature myostatin rather than total myostatin is negatively associated with bone mineral density in Chinese
    Authors: LF Wu, DC Zhu, BH Wang, YH Lu, P He, YH Zhang, HQ Gao, XW Zhu, W Xia, H Zhu, XB Mo, X Lu, L Zhang, YH Zhang, FY Deng, SF Lei
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Human
    Sample Type: Plasma
  8. Myostatin and adipokines: The role of the metabolically unhealthy obese phenotype in muscle function and aerobic capacity in young adults
    Authors: LP Carvalho, RP Basso-Vane, L Di Thommaz, RG Mendes, MC Oliveira-J, RP Vieira, JC Bonjorno-J, CR Oliveira, R Luporini, A Borghi-Sil
    Cytokine, 2017;0(0):.
    Species: Human
    Sample Type: Plasma
  9. Serum Biomarkers of Myocardial Remodeling and Coronary Dysfunction in Early Stages of Hypertrophic Cardiomyopathy in the Young
    Authors: E Fernlund, T Gyllenhamm, R Jablonowsk, M Carlsson, A Larsson, J Ärnlöv, P Liuba
    Pediatr Cardiol, 2017;0(0):.
    Species: Human
    Sample Type: Serum
  10. Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets.
    Authors: Rao S, Fujimura T, Matsunari H, Sakuma T, Nakano K, Watanabe M, Asano Y, Kitagawa E, Yamamoto T, Nagashima H
    Mol Reprod Dev, 2016;83(1):61-70.
    Species: Porcine
    Sample Type: Tissue Homogenates
  11. Serum GDF-8 levels change dynamically during controlled ovarian hyperstimulation in patients undergoing IVF/ICSI-ET
    Authors: Lanlan Fang
    Sci Rep, 2016;6(0):28036.
    Species: Human
    Sample Type: Serum
  12. Decreasing maternal myostatin programs adult offspring bone strength in a mouse model of osteogenesis imperfecta
    Proc. Natl. Acad. Sci. U.S.A., 2016;0(0):.
    Species: Mouse
    Sample Type: Serum
  13. Supplementation of Magnolol Attenuates Skeletal Muscle Atrophy in Bladder Cancer-Bearing Mice Undergoing Chemotherapy via Suppression of FoxO3 Activation and Induction of IGF-1.
    Authors: Chen M, Chen Y, Lee C, Hung C, Chou T
    PLoS ONE, 2015;10(11):e0143594.
    Species: Mouse
    Sample Type: Tissue Homogenates
  14. Relationship between Blood Myostatin Levels and Kidney Function:Shimane CoHRE Study.
    Authors: Yano S, Nagai A, Isomura M, Yamasaki M, Kijima T, Takeda M, Hamano T, Nabika T
    PLoS ONE, 2015;10(10):e0141035.
    Species: Human
    Sample Type: Plasma
  15. Preliminary investigation into a potential role for myostatin and its receptor (ActRIIB) in lean and obese horses and ponies.
    Authors: Morrison P, Bing C, Harris P, Maltin C, Grove-White D, Argo C
    PLoS ONE, 2014;9(11):e112621.
    Species: Equine
    Sample Type: Serum
  16. Myostatin regulates energy homeostasis in the heart and prevents heart failure.
    Authors: Biesemann N, Mendler L, Wietelmann A, Hermann S, Schafers M, Kruger M, Boettger T, Borchardt T, Braun T
    Circ Res, 2014;115(2):296-310.
    Species: Mouse
    Sample Type: Serum
Expand to show all Citations

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Sample Activation Kit 1

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Quantikine Immunoassay Control Set 794 Human GDF-8/Myostatin

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Average Rating: 5 (Based on 2 reviews)

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Images Ratings Applications Species Reviewed By Date Details
 GDF-8/Myostatin Quantikine ELISA Kit DGDF80 Array DGDF80
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Excellent
  Anonymous 02/20/2018
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Image Details
 GDF-8/Myostatin Quantikine ELISA Kit DGDF80
: GDF-8/Myostatin Quantikine ELISA Kit [DGDF80].

Summary

Sample Tested Serum,Mouse Serum,Human Serum

Other Experimental Details

Other Experimental Details Great kit overall. Standard curve is very reproducible (R2>0.999), and good above suggested limits. Easy to follow protocol. Great, detailed suggestion on sample dilutions and expected ranges for different samples.
 GDF-8/Myostatin Quantikine ELISA Kit DGDF80 Array DGDF80
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Excellent
  Anonymous 01/31/2018
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Image Details
 GDF-8/Myostatin Quantikine ELISA Kit DGDF80
: GDF-8/Myostatin Quantikine ELISA Kit [DGDF80].

Summary

Sample Tested Heparin Plasma,EDTA Plasma,Serum

Other Experimental Details

Other Experimental Details Ran assay successfully with human plasma (Heparin and EDTA) and human serum (SST) samples, with and without binding protein separation step. High linearity of standard curve (example shown produced by undergrad researchers) when run in triplicate.

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