GFAP Antibody (GA-5)

Novus Biologicals | Catalog # NBP2-29415

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Alpaca, Bovine, Canine, Chicken, Equine, Feline, Rabbit

Cited:

Human, Mouse, Rat, Primate

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # GA-5
View all GFAP Primary Antibodies »

Product Specifications

Immunogen

GFAP isolated from pig spinal cord (Uniprot: P14136)

Reactivity Notes

Alpaca, Feline, Canine, and Equine reactivity reported from a verified customer review.

Localization

Cytoplasmic

Marker

Astrocyte & Neural Stem Cell Marker

Specificity

This monoclonal antibody recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33184)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for GFAP Antibody (GA-5)

Immunocytochemistry/ Immunofluorescence: GFAP Antibody (GA-5) [NBP2-29415]

Immunocytochemistry/ Immunofluorescence: GFAP Antibody (GA-5) [NBP2-29415]

Immunocytochemistry/Immunofluorescence: GFAP Antibody (GA-5) [NBP2-29415] - Cultured Rat Hippocampal Neurons. ICC/IF image submitted by a verified customer review.
Western Blot: GFAP Antibody (GA-5) [NBP2-29415]

Western Blot: GFAP Antibody (GA-5) [NBP2-29415]

Western Blot: GFAP Antibody (GA-5) [NBP2-29415] - Western Blot Analysis of human brain tissue lysate using GFAP Antibody (GA-5).
Immunohistochemistry-Paraffin: GFAP Antibody (GA-5) [NBP2-29415]

Immunohistochemistry-Paraffin: GFAP Antibody (GA-5) [NBP2-29415]

Immunohistochemistry-Paraffin: GFAP Antibody (GA-5) [NBP2-29415] - Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Antibody (GA-5).
Flow Cytometry: GFAP Antibody (GA-5) [NBP2-29415]

Flow Cytometry: GFAP Antibody (GA-5) [NBP2-29415]

Flow Cytometry: GFAP Antibody (GA-5) [NBP2-29415] - Flow Cytometric Analysis of T98G cells using GFAP Antibody (GA-5) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Western Blot: GFAP Antibody (GA-5) [NBP2-29415]

Western Blot: GFAP Antibody (GA-5) [NBP2-29415]

Western Blot: GFAP Antibody (GA-5) [NBP2-29415] - Analysis of GFAP in human brain lysate using GFAP (GA5) antibody at 1 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: GFAP Antibody (GA-5) [NBP2-29415]

Immunohistochemistry-Paraffin: GFAP Antibody (GA-5) [NBP2-29415]

Immunohistochemistry-Paraffin: GFAP Antibody (GA-5) [NBP2-29415] - Formalin-paraffin human brain stained with GFAP Ab (GA-5). Note cytoplasmic staining.
Flow Cytometry: GFAP Antibody (GA-5) [NBP2-29415]

Flow Cytometry: GFAP Antibody (GA-5) [NBP2-29415]

Flow Cytometry: GFAP Antibody (GA-5) [NBP2-29415] - Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and mononuclear cells were isolated from the CNS at day 10 (onset of symptoms). Cells were stained for GFAP, Neun, CX3CL1, CXCL12, CCL2, CD45 and CD11b, plus for viability to exclude dead cells. GFAP staining is shown for viable cells. Flow cytometry image submitted by a verified customer review.
Simple Western: GFAP Antibody (GA-5) [NBP2-29415]

Simple Western: GFAP Antibody (GA-5) [NBP2-29415]

Simple Western: GFAP Antibody (GA-5) [NBP2-29415] - Simple Western lane view shows a specific band for GFAP in 0.2 mg/mL of human brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: GFAP Antibody (GA-5) [NBP2-29415]

Simple Western: GFAP Antibody (GA-5) [NBP2-29415]

Simple Western: GFAP Antibody (GA-5) [NBP2-29415] - Electropherogram image of the corresponding Simple Western lane. GFAP antibody was used at 10 ug/mL dilution of human brain lysate.
GFAP Antibody (GA-5)

Immunohistochemistry-Paraffin: Mouse Monoclonal GFAP Antibody (GA-5) [NBP2-29415] -

Immunohistochemistry-Paraffin: Mouse Monoclonal GFAP Antibody (GA-5) [NBP2-29415] - Analysis of GFAP on adult human brain tissue. Antigen retrieval in a basic buffer x200 (1ug/mL). Image from a verified customer review.
GFAP Antibody (GA-5)

Immunocytochemistry/ Immunofluorescence: GFAP Antibody (GA-5) [NBP2-29415] -

Immunocytochemistry/ Immunofluorescence: GFAP Antibody (GA-5) [NBP2-29415] - Advancing super‐resolution microscopy for astroglial research. (A) STED images showing CA1 stratum radiatum astrocytic processes (green, whole‐cell loading with Alexa Fluor 488) adjacent to synaptic structures in organotypic slices (red; Thy1‐YFP; dendritic spines, arrows) at lower (A1) & higher (A2, area indicted by square in A1) magnification; asterisk, O‐ring structures indicating tentative cell process envelopes; adapted from (Panatier et al., 2014). (B) STED image of P2Y1 receptors (red) along a multi‐branched astrocytic process (glutamine synthase, grey) in the adult mouse hippocampus. Adapted from (Volterra et al., 2014). (C) STORM imaging of pre‐ (Bassoon; red) & postsynaptic (Homer1; green) scaffolding proteins in the mouse main olfactory bulb glomeruli imaged using conventional fluorescence imaging (C1) & STORM (C2); adapted from (Dani et al., 2010). (D, E) dSTORM images of cultured (14 DIV) mixed glial cells from rat hippocampus (ProLong Diamond in Zeiss Elyra PS.1 microscope; Fiji Plugin ThunderSTORM, 3,000 frames); unpublished data by J. Heller. (D) GFAP stained with monoclonal antibody (Novus, GA5, secondary Alexa Fluor 488 donkey anti‐mouse antibody, Life Technologies) shown at lower (D1) & higher (D2, fragment indicated in D1) magnification. (E) GLT‐1 (polyclonal, Millipore) visualized with Alexa Fluor 568 goat anti‐guinea pig antibody (Life Technologies) shown at lower (E1) & higher (E2, fragment indicated in E1) magnification. Note that the cells were permeabilized, & therefore GLT‐1 was stained throughout cellular compartments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25782611), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GFAP Antibody (GA-5)

Immunohistochemistry-Paraffin - Mouse Monoclonal GFAP Antibody (GA-5) [NBP2-29415]

Images demonstrating GFAP immunoreactivity in a variety of human FFPE tissue sections. NBP2-29415 was used at a concentration of 125ng/mL and was left on tissue sections for 30m at room temperature. Heat induced epitope retrieval with a citrate-based buffer was used. Image from a verified customer review.
GFAP Antibody (GA-5)

Immunohistochemistry-Paraffin: Mouse Monoclonal GFAP Antibody (GA-5) [NBP2-29415]

Images demonstrating GFAP immunoreactivity in a variety of horse FFPE tissue sections. NBP2-29415 was used at a concentration of 125ng/mL and was left on tissue sections for 30m at room temperature. Heat induced epitope retrieval with a citrate-based buffer was used. Image from a verified customer review.
GFAP Antibody (GA-5)

Immunohistochemistry-Paraffin: Mouse Monoclonal GFAP Antibody (GA-5) [NBP2-29415]

Images demonstrating GFAP immunoreactivity in a variety of dog FFPE tissue sections. NBP2-29415 was used at a concentration of 125ng/mL and was left on tissue sections for 30m at room temperature. Heat induced epitope retrieval with a citrate-based buffer was used. Image from a verified customer review.
GFAP Antibody (GA-5)

Immunohistochemistry-Paraffin: Mouse Monoclonal GFAP Antibody (GA-5) [NBP2-29415]

Images demonstrating GFAP immunoreactivity in a variety of cat FFPE tissue sections. NBP2-29415 was used at a concentration of 125ng/mL and was left on tissue sections for 30m at room temperature. Heat induced epitope retrieval with a citrate-based buffer was used. Image from a verified customer review.
GFAP Antibody (GA-5)

Immunohistochemistry-Paraffin: Mouse Monoclonal GFAP Antibody (GA-5) [NBP2-29415]

Images demonstrating GFAP immunoreactivity in a variety of alpaca FFPE tissue sections. NBP2-29415 was used at a concentration of 125ng/mL and was left on tissue sections for 30m at room temperature. Image from a verified customer review.
GFAP Antibody (GA-5)

Immunohistochemistry-Paraffin: Mouse Monoclonal GFAP Antibody (GA-5) [NBP2-29415]

GFAP immunoreactivity in an FFPE section of cow brain. NBP2-29415 was diluted to 125ng/mL and was left on tissue sections for 30min at room temperature. Image from a verified customer review.

Applications for GFAP Antibody (GA-5)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Frozen

0.5 - 1 ug/mL

Immunohistochemistry-Paraffin

1-2 ug/ml

Simple Western

10 ug/mL

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2 ug/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (cryosections): see Tobin et. al. for details.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in h. Brain lysate(s), separated by Size, antibody dilution of 10 ug/mL, apparent MW was 54 kDa

Reviewed Applications

Read 9 reviews rated 4.8 using NBP2-29415 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: GFAP

Glial fibrillary acidic protein (GFAP) is a class III intermediate filament protein that is largely expressed in astrocytes in addition to non-myelinating Schwann cells and glial cells (1,2). Other members of the type III intermediate filament family include desmin, peripherin, and vimentin (2-4). GFAP was first identified in the brains of multiple sclerosis patients (2). Human GFAP protein is 432 amino acids in length with a theoretical molecular weight of ~50 kDa (1,3). GFAP has at least 10 known isoforms, with the most prevalent and common in the brain being GFAP-alpha which is made of a head domain, a rod domain with four coils (1A, 1B, 2A, 2B) joined by linker regions, and a tail domain (1). GFAP is a marker of mature astrocytes, but is also expressed throughout development in both fetal and adult neural stem cells (2). While the exact function of GFAP is still elusive, it has been shown to play a role in cellular processes such as migration, mitosis, structural integrity, and signaling (2).

An increase in GFAP levels is often associated with neuroinflammation which results in the activation and proliferation of astroglia cell population (1,2). GFAP expression is also observed in brains of patients with neurodegenerative diseases including Alzheimer's and Parkinson's, epilepsy disorders, and brain injuries (1-4). Lesion sites associated with neurodegeneration can exhibit an array of gliosis characteristics from glial scarring with reduced astrocyte proliferation to activated, GFAP-positive astrocytes surrounding amyloid plaques (2). Furthermore, the GFAP gene is a target of single nucleotide polymorphisms in the coding region, considered a gain-of-function mutation, characterized by astrocytic inclusions, termed Rosenthal fibers, resulting in Alexander Disease (1-4). GFAP is also a center of many post-translational modifications, such as phosphorylation, which can alter various aspects of filament assembly (1,4).

References

1. Yang, Z., & Wang, K. K. (2015). Glial fibrillary acidic protein: from intermediate filament assembly and gliosis to neurobiomarker. Trends in Neurosciences. https://doi.org/10.1016/j.tins.2015.04.003

2. Hol, E. M., & Capetanaki, Y. (2017). Type III Intermediate Filaments Desmin, Glial Fibrillary Acidic Protein (GFAP), Vimentin, and Peripherin. Cold Spring Harbor Perspectives in Biology. https://doi.org/10.1101/cshperspect.a021642

3. Potokar, M., Morita, M., Wiche, G., & Jorgacevski, J. (2020). The Diversity of Intermediate Filaments in Astrocytes. Cells. https://doi.org/10.3390/cells9071604

4. Viedma-Poyatos, a., Pajares, M. A., & Perez-Sala, D. (2020). Type III intermediate filaments as targets and effectors of electrophiles and oxidants. Redox Biology. https://doi.org/10.1016/j.redox.2020.101582

Long Name

Glial Fibrillary Acidic Protein

Alternate Names

ALXDRD, FLJ45472, GFAP, GFAP astrocytes, glial fibrillary acidic protein, GFAP elisa, GFAP flow cytometry

Gene Symbol

GFAP

UniProt

P14136

Additional GFAP Products

Product Documents for GFAP Antibody (GA-5)

Certificate of Analysis

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Product Specific Notices for GFAP Antibody (GA-5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GFAP Antibody (GA-5)

Customer Reviews for GFAP Antibody (GA-5) (9)

4.8 out of 5
9 Customer Ratings
5 Stars
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Customer Images

Showing  1 - 5 of 9 reviews Showing All
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  • GFAP Antibody (GA-5)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Brain
    Species: Cow
    Verified Customer | Posted 07/02/2025
    GFAP immunoreactivity in an FFPE section of cow brain. NBP2-29415 was diluted to 125ng/mL and was left on tissue sections for 30min at room temperature.
    GFAP Antibody (GA-5) NBP2-29415
  • GFAP Antibody (GA-5)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Multiple tissues
    Species: Human
    Verified Customer | Posted 06/03/2025
    Images demonstrating GFAP immunoreactivity in a variety of human FFPE tissue sections. NBP2-29415 was used at a concentration of 125ng/mL and was left on tissue sections for 30m at room temperature.
    Heat induced epitope retrieval with a citrate-based buffer was used.
    GFAP Antibody (GA-5) NBP2-29415
  • GFAP Antibody (GA-5)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Multiple tissues
    Species: Horse
    Verified Customer | Posted 06/03/2025
    Images demonstrating GFAP immunoreactivity in a variety of horse FFPE tissue sections. NBP2-29415 was used at a concentration of 125ng/mL and was left on tissue sections for 30m at room temperature.
    Heat induced epitope retrieval with a citrate-based buffer was used.
    GFAP Antibody (GA-5) NBP2-29415
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • GFAP Antibody (GA-5)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Multiple tissues
    Species: Dog
    Verified Customer | Posted 06/03/2025
    Images demonstrating GFAP immunoreactivity in a variety of dog FFPE tissue sections. NBP2-29415 was used at a concentration of 125ng/mL and was left on tissue sections for 30m at room temperature.
    Heat induced epitope retrieval with a citrate-based buffer was used.
    GFAP Antibody (GA-5) NBP2-29415
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • GFAP Antibody (GA-5)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Multiple tissues
    Species: Cat
    Verified Customer | Posted 06/03/2025
    Images demonstrating GFAP immunoreactivity in a variety of cat FFPE tissue sections. NBP2-29415 was used at a concentration of 125ng/mL and was left on tissue sections for 30m at room temperature.
    Heat induced epitope retrieval with a citrate-based buffer was used.
    GFAP Antibody (GA-5) NBP2-29415
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • GFAP Antibody (GA-5)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Multiple tissues
    Species: Alpaca
    Verified Customer | Posted 06/03/2025
    Images demonstrating GFAP immunoreactivity in a variety of alpaca FFPE tissue sections. NBP2-29415 was used at a concentration of 125ng/mL and was left on tissue sections for 30m at room temperature.
    GFAP Antibody (GA-5) NBP2-29415
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • GFAP Antibody (GA-5)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Adult brain
    Species: Human
    Verified Customer | Posted 09/14/2023
    astrocytes were positive for GFAP (GA-5)
    antigen retrieval in a basic buffer x200 (1ug/mL) BOND refine kit
    GFAP Antibody (GA-5) NBP2-29415
  • GFAP Antibody (GA5)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Embryonic cortical glial cells
    Species: Mouse
    Verified Customer | Posted 09/11/2017
  • GFAP Antibody (GA5)
    Name: Janosch Heller
    Application: Immunocytochemistry
    Sample Tested: Cultured rat hippocampal neurons
    Species: Rat
    Verified Customer | Posted 03/27/2017
    Fixation: 3% formaldehyde (TAAB) in PBS, 15 minutes RT Blocking: 10% donkey serum in PBS, 1 hour RT Permeabilization: 0.2% Triton-X100 Primary Antibody: 1:100 in 5% horse serum in PBS with 0.2% Triton, overnight, 4°C Wash: three times wash with PBS with 0.2% Triton, 10 min each Secondary Antibody: Invitrogen goat anti-mouse Alexa568, 1:500 in PBS with 0.2% Triton, 2 hours, RT Wash: three times wash with PBS, 10 min each Microscope: Confocal (Zeiss LSM710) The antibody labeld intermediate filaments in cultured astrocytes.
    GFAP Antibody (GA-5) NBP2-29415

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