GFAP Antibody (GA-5) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-33184

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Alpaca, Bovine, Canine, Chicken, Equine, Feline, Rabbit

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Frozen, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # GA-5

Format

Azide and BSA Free
View all GFAP Primary Antibodies »

Product Specifications

Immunogen

GFAP isolated from pig spinal cord (Uniprot: P14136)

Reactivity Notes

Alpaca, Feline, Canine, and Equine reactivity reported from a verified customer review.

Localization

Cytoplasmic

Marker

Astrocyte & Neural Stem Cell Marker

Specificity

This monoclonal antibody recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29415).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for GFAP Antibody (GA-5) - Azide and BSA Free

Western Blot: GFAP Antibody (GA-5)Azide and BSA Free [NBP2-33184]

Western Blot: GFAP Antibody (GA-5)Azide and BSA Free [NBP2-33184]

Western Blot: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184] - Analysis of GFAP in human brain lysate using GFAP (GA5) antibody at 1 ug/mL. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunocytochemistry/ Immunofluorescence: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184]

Immunocytochemistry/ Immunofluorescence: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184]

Immunocytochemistry/Immunofluorescence: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184] - Cultured Rat Hippocampal Neurons. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184]

Immunohistochemistry-Paraffin: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184]

Immunohistochemistry-Paraffin: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184] - Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Antibody (GA-5).
Flow Cytometry: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184]

Flow Cytometry: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184]

Flow Cytometry: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184] - Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and mononuclear cells were isolated from the CNS at day 10 (onset of symptoms). Cells were stained for GFAP, Neun, CX3CL1, CXCL12, CCL2, CD45 and CD11b, plus for viability to exclude dead cells. GFAP staining is shown for viable cells. Flow cytometry image submited by a verified customer review.
Western Blot: GFAP Antibody (GA-5)Azide and BSA Free [NBP2-33184]

Western Blot: GFAP Antibody (GA-5)Azide and BSA Free [NBP2-33184]

Western Blot: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184] - Analysis showing relative size.
Western Blot: GFAP Antibody (GA-5)Azide and BSA Free [NBP2-33184]

Western Blot: GFAP Antibody (GA-5)Azide and BSA Free [NBP2-33184]

Western Blot: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184] - Western Blot Analysis of human brain tissue lysate using GFAP Antibody (GA-5).
Immunohistochemistry-Paraffin: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184]

Immunohistochemistry-Paraffin: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184]

Immunohistochemistry-Paraffin: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184] - Analysis in human Cerebellum.
Flow Cytometry: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184]

Flow Cytometry: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184]

Flow Cytometry: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184] - Flow Cytometric Analysis of T98G cells using GFAP Antibody (GA-5) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Simple Western: GFAP Antibody (GA-5)Azide and BSA Free [NBP2-33184]

Simple Western: GFAP Antibody (GA-5)Azide and BSA Free [NBP2-33184]

Simple Western: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184] - Simple Western lane view shows a specific band for GFAP in 0.2 mg/mL of human brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: GFAP Antibody (GA-5)Azide and BSA Free [NBP2-33184]

Simple Western: GFAP Antibody (GA-5)Azide and BSA Free [NBP2-33184]

Simple Western: GFAP Antibody (GA-5) - Azide and BSA Free [NBP2-33184] - Electropherogram image of the corresponding Simple Western lane. GFAP antibody was used at 10 ug/mL dilution of human brain lysates.

Applications for GFAP Antibody (GA-5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2 ug/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
See Simple Western Antibody Database for Simple Western validation

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: GFAP

Glial fibrillary acidic protein (GFAP) is a class III intermediate filament protein that is largely expressed in astrocytes in addition to non-myelinating Schwann cells and glial cells (1,2). Other members of the type III intermediate filament family include desmin, peripherin, and vimentin (2-4). GFAP was first identified in the brains of multiple sclerosis patients (2). Human GFAP protein is 432 amino acids in length with a theoretical molecular weight of ~50 kDa (1,3). GFAP has at least 10 known isoforms, with the most prevalent and common in the brain being GFAP-alpha which is made of a head domain, a rod domain with four coils (1A, 1B, 2A, 2B) joined by linker regions, and a tail domain (1). GFAP is a marker of mature astrocytes, but is also expressed throughout development in both fetal and adult neural stem cells (2). While the exact function of GFAP is still elusive, it has been shown to play a role in cellular processes such as migration, mitosis, structural integrity, and signaling (2).

An increase in GFAP levels is often associated with neuroinflammation which results in the activation and proliferation of astroglia cell population (1,2). GFAP expression is also observed in brains of patients with neurodegenerative diseases including Alzheimer's and Parkinson's, epilepsy disorders, and brain injuries (1-4). Lesion sites associated with neurodegeneration can exhibit an array of gliosis characteristics from glial scarring with reduced astrocyte proliferation to activated, GFAP-positive astrocytes surrounding amyloid plaques (2). Furthermore, the GFAP gene is a target of single nucleotide polymorphisms in the coding region, considered a gain-of-function mutation, characterized by astrocytic inclusions, termed Rosenthal fibers, resulting in Alexander Disease (1-4). GFAP is also a center of many post-translational modifications, such as phosphorylation, which can alter various aspects of filament assembly (1,4).

References

1. Yang, Z., & Wang, K. K. (2015). Glial fibrillary acidic protein: from intermediate filament assembly and gliosis to neurobiomarker. Trends in Neurosciences. https://doi.org/10.1016/j.tins.2015.04.003

2. Hol, E. M., & Capetanaki, Y. (2017). Type III Intermediate Filaments Desmin, Glial Fibrillary Acidic Protein (GFAP), Vimentin, and Peripherin. Cold Spring Harbor Perspectives in Biology. https://doi.org/10.1101/cshperspect.a021642

3. Potokar, M., Morita, M., Wiche, G., & Jorgacevski, J. (2020). The Diversity of Intermediate Filaments in Astrocytes. Cells. https://doi.org/10.3390/cells9071604

4. Viedma-Poyatos, a., Pajares, M. A., & Perez-Sala, D. (2020). Type III intermediate filaments as targets and effectors of electrophiles and oxidants. Redox Biology. https://doi.org/10.1016/j.redox.2020.101582

Long Name

Glial Fibrillary Acidic Protein

Alternate Names

ALXDRD, FLJ45472, GFAP, GFAP astrocytes, glial fibrillary acidic protein

Gene Symbol

GFAP

Additional GFAP Products

Product Documents for GFAP Antibody (GA-5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GFAP Antibody (GA-5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GFAP Antibody (GA-5) - Azide and BSA Free

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