INTENDED USE & DESCRIPTION

Glo Substrate Reagent Pack contains 5 vials of Glo Reagent A (stabilized enhanced luminol, Part # 895868, 4 mL/vial) and 5 vials of Glo Reagent B (stabilized hydrogen peroxide, Part # 895869, 8 mL/vial) for use in chemiluminescent immunoassay development.

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. Use within 15 minutes to 4 hours of mixing.

PROCEDURE

This reagent may be substituted for H2O2/tetramethylbenzidine as a substrate for horseradish peroxidase in ELISAs. Use black microplates for chemiluminescent assays (Catalog # DY991). Mix 1 part Glo Reagent A to 2 parts Glo Reagent B in a plastic container, cap, and protect from light. Add 100 µL of the mixture to each well and incubate at room temperature while protecting from light. Read the microplate on a luminometer within 5-20 minutes. 

Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Sample Type
  1. First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
    Authors: Thadhani R, Powe CE, Tjoa ML
    Diabetes Care, 2010;33(3):664-9. 2010
  2. Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
    Authors: Khankin EV, Mutter WP, Tamez H
    PLoS ONE, 2010;5(2):e9246. 2010
  3. Leptin Levels Are Higher in Whole Compared to Skim Human Milk, Supporting a Cellular Contribution.
    Authors: Kugananthan S, Lai C, Gridneva Z, Mark P, Geddes D, Kakulas F
    Nutrients, 0;8(11):. 0
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Average Rating: 5 (Based on 1 review)

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