Glucagon Quantikine ELISA Kit

  (7 citations)
(1 Review)
    
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Product Details
Assay Procedure
Citations (7)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    14.7 pg/mL
  • Assay Range
    31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural Glucagon.  This assay cross-reacts <12% with Oxyntomodulin.
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC100 , Quantikine Immunoassay Control Set 869 for Glucagon - Please Inquire
Product Summary
The Quantikine Glucagon Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Glucagon in cell culture supernates, serum, and plasma. It contains natural porcine Glucagon as the standard. The antibodies were raised against a human Glucagon synthetic peptide. This immunoassay has been shown to accurately quantitate human, mouse, rat, and porcine Glucagon.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 315 618 1024 354 653 1080
Standard Deviation 10.3 22 27.6 30.7 37.8 63.6
CV% 3.3 3.6 2.7 8.7 5.8 5.9

Recovery

The recovery of Glucagon spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 91-108
Human EDTA Plasma (n=4) 98 86-107
Human Heparin Plasma (n=4) 96 88-100
Human Serum (n=4) 102 95-111
Mouse Serum (n=2) 99 92-106
Porcine Serum (n=2) 97 91-107
Rat Serum (n=1) 93 86-101
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of Glucagon were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 Glucagon [HRP]
 Glucagon [HRP]
Product Datasheets

Certificate of Analysis Lookup

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Glucagon
Glucagon is a pancreatic peptide hormone that plays a critical role in glucose metabolism and homeostasis. Mature Glucagon is generated from its precursor by the prohormone convertase PC2/PCSK2 in pancreatic alpha-cells. Glucagon is secreted in response to low blood glucose (hypoglycemia) and downregulated in response to high blood glucose (hyperglycemia). Glucagon production is under the control of both hormones and neurotransmitters. Glucagon inhibits Insulin secretion, and vice versa. Glucagon stimulates hepatic gluconeogenesis and glycogenolysis, thereby increasing blood glucose. Like insulin, Glucagon is dysregulated in type 2 diabetes (T2D) and contributes to its pathology.
  • Entrez Gene IDs:
    2641 (Human); 14526 (Mouse); 24952 (Rat)
  • Alternate Names:
    GCG; glicentin-related polypeptide; Glucagon; GRPP
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

The conjugate must be kept cold during use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
  3.   Wash and aspirate the plate a total of 2 times with Wash Buffer prior to assay.

  4. 150 µL Assay Diluent
  5.   Add 150 µL of Assay Diluent to each well.

  6. 50 µL Standard, Control, or Sample
  7.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
  8.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  9. 200 µL cold Conjugate
  10.   Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour.
  11.   Aspirate and wash 4 times.

  12. 200 µL Substrate Solution
  13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  14. 50 µL Stop Solution
  15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Sample Type
  1. Involvement of stanniocalcins in the deregulation of glycaemia in obese mice and type 2 diabetic patients
    Authors: JJ López, I Jardín, CC Chamorro, ML Duran, MJ Tarancón R, M Reyes Pana, F Jiménez, R Montero, MJ González, M Martínez, MJ Hernández, JM Brull, AJ Corbacho, E Delgado, MP Granados, L Gómez-Gord, JA Rosado, PC Redondo
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Mouse
    Sample Type: Plasma
  2. Metabolic regulation of GLP-1 and PC1/3 in pancreatic ?-cell line
    Authors: V Sancho, G Daniele, D Lucchesi, R Lupi, A Ciccarone, G Penno, C Bianchi, A Dardano, R Miccoli, S Del Prato
    PLoS ONE, 2017;12(11):e0187836.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  3. Effects of Three Commercially Available Sports Drinks on Substrate Metabolism and Subsequent Endurance Performance in a Postprandial State
    Authors: L Qin, QR Wang, ZL Fang, T Wang, AQ Yu, YJ Zhou, Y Zheng, MQ Yi
    Nutrients, 2017;9(4):.
    Species: Human
    Sample Type: Serum
  4. Vitamin A deficiency causes hyperglycemia and loss of pancreatic beta-cell mass.
    Authors: Trasino S, Benoit Y, Gudas L
    J Biol Chem, 2015;290(3):1456-73.
    Species: Mouse
    Sample Type: Serum
  5. Inhibition of the glucose transporter SGLT2 with dapagliflozin in pancreatic alpha cells triggers glucagon secretion.
    Authors: Bonner C, Kerr-Conte J, Gmyr V, Queniat G, Moerman E, Thevenet J, Beaucamps C, Delalleau N, Popescu I, Malaisse W, Sener A, Deprez B, Abderrahmani A, Staels B, Pattou F
    Nat Med, 2015;21(5):512-7.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. A novel oral dual amylin and calcitonin receptor agonist (KBP-042) exerts antiobesity and antidiabetic effects in rats.
    Authors: Andreassen K, Feigh M, Hjuler S, Gydesen S, Henriksen J, Beck-Nielsen H, Christiansen C, Karsdal M, Henriksen K
    Am J Physiol Endocrinol Metab, 2014;307(1):E24-33.
    Species: Rat
    Sample Type: Plasma
  7. Hypothalamic prolyl endopeptidase (PREP) regulates pancreatic insulin and glucagon secretion in mice.
    Authors: Kim J, Toda C, D'Agostino G, Zeiss C, DiLeone R, Elsworth J, Kibbey R, Chan O, Harvey B, Richie C, Savolainen M, Myohanen T, Jeong J, Diano S
    Proc Natl Acad Sci U S A, 2014;111(32):11876-81.
    Species: Mouse
    Sample Type: Tissue Homogenates

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ELISA Controls
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Quantikine Immunoassay Control Set 869 for Glucagon

Ctrl QC100
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Supplemental ELISA Products
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Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
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Average Rating: 5 (Based on 1 review)

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