Natural Glucagon. This assay cross-reacts <12% with Oxyntomodulin.
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.
No significant interference observed with available related molecules.
The Quantikine Glucagon Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Glucagon in cell culture supernates, serum, and plasma. It contains natural porcine Glucagon as the standard. The antibodies were raised against a human Glucagon synthetic peptide. This immunoassay has been shown to accurately quantitate human, mouse, rat, and porcine Glucagon.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of Glucagon spiked to levels throughout the range of the assay in various matrices was evaluated.
Average % Recovery
Cell Culture Media (n=4)
Human EDTA Plasma (n=4)
Human Heparin Plasma (n=4)
Human Serum (n=4)
Mouse Serum (n=2)
Porcine Serum (n=2)
Rat Serum (n=1)
To assess the linearity of the assay, samples spiked with high concentrations of Glucagon were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Glucagon is a pancreatic peptide hormone that plays a critical role in glucose metabolism and homeostasis. Mature Glucagon is generated from its precursor by the prohormone convertase PC2/PCSK2 in pancreatic alpha-cells. Glucagon is secreted in response to low blood glucose (hypoglycemia) and downregulated in response to high blood glucose (hyperglycemia). Glucagon production is under the control of both hormones and neurotransmitters. Glucagon inhibits Insulin secretion, and vice versa. Glucagon stimulates hepatic gluconeogenesis and glycogenolysis, thereby increasing blood glucose. Like insulin, Glucagon is dysregulated in type 2 diabetes (T2D) and contributes to its pathology.
Refer to the product for complete assay procedure.
The conjugate must be kept cold during use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
Prepare all reagents, standard dilutions, and samples as directed in the product insert.
Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
Wash and aspirate the plate a total of 2 times with Wash Buffer prior to assay.
150 µL Assay Diluent
Add 150 µL of Assay Diluent to each well.
50 µL Standard, Control, or Sample
Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
200 µL cold Conjugate
Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour.
Aspirate and wash 4 times.
200 µL Substrate Solution
Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
50 µL Stop Solution
Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.