Glucosylceramidase/GBA Antibody (2E2) - Azide and BSA Free

Novus Biologicals | Catalog # H00002629-M01

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Plant

Cited:

Human, Plant

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E2

Format

Azide and BSA Free
View all Glucosylceramidase/GBA Primary Antibodies »

Product Specifications

Immunogen

GBA (NP_000148, 146 a.a. ~ 235 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGS

Reactivity Notes

Plant reactivity reported in scientific literature (PMID: 22592100).

Specificity

GBA - glucosidase, beta; acid (includes glucosylceramidase)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Glucosylceramidase/GBA Antibody (2E2) - Azide and BSA Free

Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01]

Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01]

Glucosylceramidase-GBA-Antibody-2E2-Western-Blot-H00002629-M01-img0019.jpg
Immunocytochemistry/ Immunofluorescence: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01]

Immunocytochemistry/ Immunofluorescence: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01]

Immunocytochemistry/Immunofluorescence: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of monoclonal antibody to GBA on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01]

Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01]

Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of monoclonal antibody to GBA on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 3 ug/ml.
Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01]

Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01]

Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - GBA monoclonal antibody (M01), clone 2E2 Analysis of GBA expression in MCF-7.
Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01]

Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01]

Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of GBA expression in transfected 293T cell line by GBA monoclonal antibody (M01), clone 2E2.Lane 1: GBA transfected lysate(60 KDa).Lane 2: Non-transfected lysate.
ELISA: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01]

ELISA: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01]

ELISA: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Detection limit for recombinant GST tagged GBA is approximately 1ng/ml as a capture antibody.

Applications for Glucosylceramidase/GBA Antibody (2E2) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10ug/ml

Immunohistochemistry

3ug/ml

Immunohistochemistry-Paraffin

3ug/ml

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein, transfected lysate and cell lysate for WB. It has been used for IF, IHC-P and ELISA.

Reviewed Applications

Read 1 review rated 5 using H00002629-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glucosylceramidase/GBA

This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants encoding the same protein.

Alternate Names

Alglucerase, GBA, GBA1, Imiglucerase

Entrez Gene IDs

2629 (Human)

Gene Symbol

GBA

OMIM

230 (Human)

UniProt

NP_000148 (Human)

Additional Glucosylceramidase/GBA Products

Product Documents for Glucosylceramidase/GBA Antibody (2E2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Glucosylceramidase/GBA Antibody (2E2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glucosylceramidase/GBA Antibody (2E2) - Azide and BSA Free

Customer Reviews for Glucosylceramidase/GBA Antibody (2E2) - Azide and BSA Free (1)

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  • Glucosylceramidase/GBA Antibody (2E2)
    Name: Steven Zhou
    Application: Western Blot
    Sample Tested: mouse liver, Mouse Spleen and Mouse brain
    Species: ansgenic mice
    Verified Customer | Posted 10/10/2016
    Glucosylceramidase/GBA Antibody (2E2) - Azide and BSA Free H00002629-M01

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