Glut4 Antibody [Alexa Fluor® 647]

Novus Biologicals | Catalog # NBP1-49533AF647

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Plant

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Polyclonal Rabbit IgG
View all Glut4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from the human Glucose Transporter GLUT4 protein [UniProt P14672]

Reactivity Notes

Plant reactivity reported in scientific literature (PMID: 31555030).

Localization

Endomembrane system; Multi-pass membrane protein. Cytoplasm - perinuclear region. Note: Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glut4 Antibody [Alexa Fluor® 647]

Flow Cytometry: Glut4 Antibody [Alexa Fluor® 647] [NBP1-49533AF647] - Analysis of Glut4 antibody on mouse Thymic Epithelial cells. Glut4 antibody in comparison to negative and IgG Control. Primary antibody dilution: 1:500. Image from verified customer review.
Flow Cytometry: Glut4 Antibody [Alexa Fluor (R) 647] [NBP1-49533AF647] - FLOW analysis of Glut 4 expression on Murine CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. This Alexa Fluor (R) 647 conjugated Glut 4 antibody (orange) positively stained mouse's CD4+ T cells compared to Isotype Control (Rb IgG AF647, Novus NBP2-36463AF647, blue) and fluorescence minus one/FMO control (red) [Image submitted by Verified Customer - see Product Review for Protocol Details].

Applications for Glut4 Antibody [Alexa Fluor® 647]

Application
Recommended Usage

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-49533AF647 in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Glut4

The stimulation of glucose uptake by insulin in muscle and adipose tissue requires translocation of the GLUT4 glucose transporter from intracellular storage sites to the cell surface. Activation of phosphatidylinositol-3-OH kinase (PI3K) is required for this trafficking event, but it is not sufficient to produce GLUT4 translocation. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.

Alternate Names

SLC2A4

Gene Symbol

SLC2A4

Additional Glut4 Products

Product Documents for Glut4 Antibody [Alexa Fluor® 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Glut4 Antibody [Alexa Fluor® 647]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glut4 Antibody [Alexa Fluor® 647]

Customer Reviews for Glut4 Antibody [Alexa Fluor® 647] (2)

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Customer Images

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  • Glut4 Antibody [Alexa Fluor® 647]
    Name: Anja Kusch
    Application: Flow Cytometry
    Sample Tested: Thymus tissue
    Species: Mouse
    Verified Customer | Posted 01/26/2023
    Glut4 Antibody in comparison to negative and IgG Control
    Use on Thymic Epithelial cells. In comparison to the negative and IgG control the Glut4 staining showed a clear staining. The fluorescence intensity could be higher though.
    Glut4 Antibody [Alexa Fluor® 647] NBP1-49533AF647
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Murine/Mouse's CD4+ T cells
    Species: Mouse
    Verified Customer | Posted 02/26/2015
    Glut 4 antibody NBP1-49533AF647 positively stained murine CD4+ T cells compared to isotype control (blue) and FMO control (red)
    Glut4 Antibody [Alexa Fluor® 647] NBP1-49533AF647

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