Glypican 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-42718

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Glypican 1 Primary Antibodies »

Product Specifications

Immunogen

Glypican 1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Glypican 1-1 protein. (Uniprot: P35052)

Reactivity Notes

A BLAST analysis was used to suggest partial cross-reactivity with Glypican 1 from rat, mouse, Macaque, dog, cattle, and opossum sources based on 100 - 88% homology with the immunizing sequence. Cross-reactivity with Glypican 1 from other sources has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Glypican 1 Antibody - BSA Free

Western Blot: Glypican 1 Antibody [NBP1-42718]

Western Blot: Glypican 1 Antibody [NBP1-42718]

Western Blot: Glypican 1 Antibody [NBP1-42718] - Lane 1: untransfected 293T cell lysate. Lane 2: 293T whole cell lysate. Load: 50ug per lane. Primary antibody: Glypican-1 antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Gt-a-Rabbit IgG diluted 1:5,000 at 4 C. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 61kDa, 61kDa for Glypican-1. Other band(s): 110kDa is likely due to the presence of the Fc-tag. Minor bands may represent post translational modifications of glypican-1.
Immunohistochemistry: Glypican 1 Antibody [NBP1-42718]

Immunohistochemistry: Glypican 1 Antibody [NBP1-42718]

Immunohistochemistry: Glypican 1 Antibody [NBP1-42718] - Tissue: skin. Fixation: formalin fixed paraffin embedded. Primary antibody: anti-Glypican antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Glypican as precipitated red signal with hematoxylin purple nuclear counterstain.

Applications for Glypican 1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:10000-1:50000

Immunohistochemistry

10ug/ml

Western Blot

1:500-1:2000
Application Notes
This product has been tested for use in ELISA, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~61 kDa in size corresponding to glypican by western blotting in the appropriate cell lysate or extract. The higher molecular weight (110kDa) of transfected Fc-glypican compared with the expected MW of glypican is likely due to the presence of the Fc-tag.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Glypican 1

Glypican-1 (also known as Glypican 1/GPC1 or FLJ38078) is a member of the glypican-related integral membrane proteoglycan family (GRIPS). This protein is a heparan sulfate proteoglycan which is composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains and are anchored to the cell surface via a covalent linkage to glycosylphosphatidlyinositol (GPI). Glypicans can modify cell signaling pathways and contribute to cellular proliferation and tissue growth. In humans, glypican-1 is over expressed in breast and brain cancers (gliomas). All glypicans contain an N-terminal signal peptide and a hydrophobic domain in their C-terminal region which is required for attachment of the GPI anchor. The amino acid sequences of the six vertebrate glypican family members vary
from 17% to 63% identity. The location of 14 cysteine amino acids is conserved between the glypicans, suggesting the existence of a highly similar three-dimensional structure. Heparan sulfate glycosaminoglycan chains are attached at the 50 amino acids at the C-terminal end of the protein, near the anchor and the cell membrane. Glypican functions as coreceptor for a variety of growth factors. Glypican 1/GPC1 has been shown to interact with SLIT2.

Alternate Names

GPC1

Entrez Gene IDs

2817 (Human)

Gene Symbol

GPC1

UniProt

P35052

Additional Glypican 1 Products

Product Documents for Glypican 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Glypican 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Proteoglycans
Wnt Signaling Modulators

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