GM-CSF Antibody

Novus Biologicals | Catalog # NB600-632

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA

Cited:

Immunohistochemistry, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum
View all GM-CSF Primary Antibodies »

Product Specifications

Immunogen

This whole rabbit serum was prepared by repeated immunizations with full length recombinant human GM-CSF. (Uniprot: P04141)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31220744).

Localization

Secreted

Specificity

In ELISA and other immunoreactive assays, this antiserum will recognize both native and recombinant human GM-CSF in cell supernatants and certain body fluids.

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Description

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Applications for GM-CSF Antibody

Application
Recommended Usage

ELISA

1:1000-1:5000

Immunohistochemistry

1:10-1:500

Western Blot

1:500-1:2000
Application Notes
This antiserum against Human GM-CSF has been tested for use in immunoblotting. Reactivity is also expected in neutralizations, radioimmunoassay, ELISA and immunohistochemistry. This antibody is useful for neutralization of human GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Antiserum

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: GM-CSF

The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Long Name

Granulocyte Macrophage Growth Factor

Alternate Names

CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim

Entrez Gene IDs

1437 (Human)

Gene Symbol

CSF2

UniProt

P04141 (Human)

Additional GM-CSF Products

Product Documents for GM-CSF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GM-CSF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GM-CSF Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for GM-CSF Antibody

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I found #NB600-632 but I worry if the applications of this antibody will be changed too. Could you confirm the antibody and let us know if it can be used for IHC and Neut?

    A: NB600-632 is guaranteed for use in IHC but not for neutralization. Both NBP2-12341 and NBP1-42357 are useful for both IHC frozen sections and neutralization applications.

  • Q: We have a customer who is interested in NB600-632 (anti-GM-CSF) for use in IHC and has some questions: 1. Are there any images available? 2. Has the antibody been specifically tested with formalin-fixed tissues? 3. Is there a product specific protocol, or if not, is there a recommended method of antigen retrieval? 4. What tissue type(s) were tested? Is there a recommended positive or negative control tissue?

    A: NB600-632 has not yet been tested for IHC, however since it has been successfully used for IHC by our customers in published papers, it is guaranteed for IHC as well. Please check the following references for more details: 1) Bayne LJ, Beatty GL, Jhala N et al. Tumor-derived granulocyte-macrophage colony-stimulating factor regulates myeloid inflammation and T cell immunity in pancreatic cancer Cell 2012 Jun 12 [PMID: 22698406] 2) Pylayeva-Gupta Y, Lee KE, Hajdu CH et al. Oncogenic Kras-Induced GM-CSF Production Promotes the Development of Pancreatic Neoplasia Cancer Cell 2012 Jun 12 [PMID: 22698407]

  • Q: I found #NB600-632 but I worry if the applications of this antibody will be changed too. Could you confirm the antibody and let us know if it can be used for IHC and Neut?

    A: NB600-632 is guaranteed for use in IHC but not for neutralization. Both NBP2-12341 and NBP1-42357 are useful for both IHC frozen sections and neutralization applications.

  • Q: We have a customer who is interested in NB600-632 (anti-GM-CSF) for use in IHC and has some questions: 1. Are there any images available? 2. Has the antibody been specifically tested with formalin-fixed tissues? 3. Is there a product specific protocol, or if not, is there a recommended method of antigen retrieval? 4. What tissue type(s) were tested? Is there a recommended positive or negative control tissue?

    A: NB600-632 has not yet been tested for IHC, however since it has been successfully used for IHC by our customers in published papers, it is guaranteed for IHC as well. Please check the following references for more details: 1) Bayne LJ, Beatty GL, Jhala N et al. Tumor-derived granulocyte-macrophage colony-stimulating factor regulates myeloid inflammation and T cell immunity in pancreatic cancer Cell 2012 Jun 12 [PMID: 22698406] 2) Pylayeva-Gupta Y, Lee KE, Hajdu CH et al. Oncogenic Kras-Induced GM-CSF Production Promotes the Development of Pancreatic Neoplasia Cancer Cell 2012 Jun 12 [PMID: 22698407]

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