Intracellular Staining by Flow Cytometry
|Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged protein by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with His tagged-protein was stained with Mouse Anti-His Tag Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # IC050N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
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