His Tag Horseradish Peroxidase-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of His Tag by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1. PVDF membrane was probed with a 1:4000 dilution of Mouse Anti-His Tag HRP-conjugated Monoclonal Antibody (Catalog # MAB050H). Specific bands were detected for His Tag at approximately 32 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of His Tag by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1, loaded at 0.2 mg/mL. Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated) using a 1:1000 dilution of Mouse Anti-His Tag HRP-conjugated Monoclonal Antibody (Catalog # MAB050H). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: His Tag
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.
Product Datasheets
Citations for His Tag Horseradish Peroxidase-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
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Evaluation of Immunogenicity and Efficacy of Fasciola hepatica Tetraspanin 2 (TSP2) Fused to E. coli Heat-Labile Enterotoxin B Subunit LTB Adjuvant Following Intranasal Vaccination of Cattle
Authors: G Zerna, VA Rathinasam, H Toet, G Anderson, R Dempster, TW Spithill, T Beddoe
Vaccines, 2021;9(11):.
Species: Yeast
Sample Types: Cell Lysates
Applications: ELISA Development -
Histone Methyltransferase DOT1L is Involved in Larval Molting and Second Stage Nymphal Feeding in Ornithodoros Moubata
Authors: J Gobl, D Kumar Sinh, R Sima, J Perner, P Kopá?ek, JJ Valdés, ROM Rego, A Cabezas-Cr
Vaccines (Basel), 2020;8(2):.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Characterization of a profilin-like protein from Fasciola hepatica
Authors: J Wilkie, TC Cameron, T Beddoe
PeerJ, 2020;8(0):e10503.
Species: Fasciolosis
Sample Types: Recombinant Protein
Applications: Western Blot -
S-nitrosylation-mediated activation of a histidine kinase represses the type 3 secretion system and promotes virulence of an enteric pathogen
Authors: D Gu, Y Zhang, Q Wang, X Zhou
Nat Commun, 2020;11(1):5777.
Species: Vibrio parahaemolyticus
Sample Types: Cell Lysates
Applications: Western Blot -
Phosphatidylinositol-3-OH kinase signalling is spatially organized at endosomal compartments by microtubule-associated protein 4
Authors: N Thapa, M Chen, HT Horn, S Choi, T Wen, RA Anderson
Nat Cell Biol, 2020;22(11):1357-1370.
Species: Human
Sample Types: Protein
Applications: Functional Assay -
A Periplasmic Antimicrobial Peptide-Binding Protein Is Required for Stress Survival in Vibrio cholerae
Authors: J Saul-McBet, JS Matson
Front Microbiol, 2019;10(0):161.
Applications: Western Blot -
Dual regulation of Arabidopsis AGO2 by arginine methylation
Authors: P Hu, H Zhao, P Zhu, Y Xiao, W Miao, Y Wang, H Jin
Nat Commun, 2019;10(1):844.
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Sensitivity of Kupffer cells and liver sinusoidal endothelial cells to ricin toxin and ricin toxin-Ab complexes
Authors: B Mooney, FJ Torres-Vel, J Doering, DJ Ehrbar, NJ Mantis
J. Leukoc. Biol., 2019;0(0):.
Species: Human
Sample Types: Protein
Applications: Binding Assay -
Functional genomic characterization of a synthetic anti-HER3 antibody reveals a role for ubiquitination by RNF41 in the anti-proliferative response
Authors: JP Turowec, EWT Lau, X Wang, KR Brown, FA Fellouse, KK Jawanda, J Pan, J Moffat, S Sidhu
J. Biol. Chem., 2018;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
CD36 Differently Regulates Macrophage Responses to Smooth and Rough Lipopolysaccharide
Authors: R Biedro?, A Peru?, S Józefowski
PLoS ONE, 2016;11(4):e0153558.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Binding Assay -
Oxidation by neutrophils-derived HOCl increases immunogenicity of proteins by converting them into ligands of several endocytic receptors involved in antigen uptake by dendritic cells and macrophages.
Authors: Biedron R, Konopinski M, Marcinkiewicz J, Jozefowski S
PLoS ONE, 2015;10(4):e0123293.
Species: Mouse
Sample Types: Protein
Applications: ELISA Development (Detection) -
Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitro.
Authors: Getmanova EV, Chen Y, Bloom L, Gokemeijer J, Shamah S, Warikoo V, Wang J, Ling V, Sun L
Chem. Biol., 2006;13(5):549-56.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Extracellular Regulation of Bone Morphogenetic Protein Activity by the Microfibril Component Fibrillin-1.
Authors: Wohl A, Troilo H, Collins R, Baldock C, Sengle G
J Biol Chem, 0;291(24):12732-46.
Species: Human
Sample Types: Protein
Reviews for His Tag Horseradish Peroxidase-conjugated Antibody
Average Rating: 5 (Based on 4 Reviews)
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Western blot was made in 5% BSA, antibody worked beautifully at 1:1000 dilution.
Excellent Antibody. Detects 6xhis positive control, almost instantaneously.