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His Tag Horseradish Peroxidase-conjugated Antibody

MAB050H 13 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB050H
MAB050H-SP
Detection of His Tag by Western Blot.
2 Images
Product Details
Citations (13)
FAQs
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

His Tag Horseradish Peroxidase-conjugated Antibody Summary

Specificity
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags at the amino- or carboxyl‑terminus.
Source
Monoclonal Mouse IgG1 Clone # AD1.1.10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
His-tagged peptide
Formulation
Supplied as a 0.2 μm filtered solution in Sodium Phosphate and Proclin 300. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
HRP(Excitation= nm, Emission= nm)

Applications

Recommended Concentration
Sample
Western Blot
1:4000 dilution
See below
Simple Western
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of His Tag antibody by Western Blot. View Larger

Detection of His Tag by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1. PVDF membrane was probed with a 1:4000 dilution of Mouse Anti-His Tag HRP-conjugated Monoclonal Antibody (Catalog # MAB050H). Specific bands were detected for His Tag at approximately 32 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of His Tag antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of His Tag by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1, loaded at 0.2 mg/mL. Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated) using a 1:1000 dilution of Mouse Anti-His Tag HRP-conjugated Monoclonal Antibody (Catalog # MAB050H). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name
Histidine Tag
Alternate Names
6 His epitope tag; 6-His Tag; 6X His Tag ; 6X His; 6x-His Tag; 6X-His; H; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His Tag; HIS; His6-Tag; polyhistidine tag; polyHistidine; Poly-histidine

Product Datasheets

Product Datasheet
COA
SDS

Citations for His Tag Horseradish Peroxidase-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Evaluation of Immunogenicity and Efficacy of Fasciola hepatica Tetraspanin 2 (TSP2) Fused to E. coli Heat-Labile Enterotoxin B Subunit LTB Adjuvant Following Intranasal Vaccination of Cattle
    Authors: G Zerna, VA Rathinasam, H Toet, G Anderson, R Dempster, TW Spithill, T Beddoe
    Vaccines, 2021;9(11):.
    Species: Yeast
    Sample Types: Cell Lysates
    Applications: ELISA Development
  2. Histone Methyltransferase DOT1L is Involved in Larval Molting and Second Stage Nymphal Feeding in Ornithodoros Moubata
    Authors: J Gobl, D Kumar Sinh, R Sima, J Perner, P Kopá?ek, JJ Valdés, ROM Rego, A Cabezas-Cr
    Vaccines (Basel), 2020;8(2):.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  3. Characterization of a profilin-like protein from Fasciola hepatica
    Authors: J Wilkie, TC Cameron, T Beddoe
    PeerJ, 2020;8(0):e10503.
    Species: Fasciolosis
    Sample Types: Recombinant Protein
    Applications: Western Blot
  4. S-nitrosylation-mediated activation of a histidine kinase represses the type 3 secretion system and promotes virulence of an enteric pathogen
    Authors: D Gu, Y Zhang, Q Wang, X Zhou
    Nat Commun, 2020;11(1):5777.
    Species: Vibrio parahaemolyticus
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Phosphatidylinositol-3-OH kinase signalling is spatially organized at endosomal compartments by microtubule-associated protein 4
    Authors: N Thapa, M Chen, HT Horn, S Choi, T Wen, RA Anderson
    Nat Cell Biol, 2020;22(11):1357-1370.
    Species: Human
    Sample Types: Protein
    Applications: Functional Assay
  6. A Periplasmic Antimicrobial Peptide-Binding Protein Is Required for Stress Survival in Vibrio cholerae
    Authors: J Saul-McBet, JS Matson
    Front Microbiol, 2019;10(0):161.
    Applications: Western Blot
  7. Dual regulation of Arabidopsis AGO2 by arginine methylation
    Authors: P Hu, H Zhao, P Zhu, Y Xiao, W Miao, Y Wang, H Jin
    Nat Commun, 2019;10(1):844.
  8. Sensitivity of Kupffer cells and liver sinusoidal endothelial cells to ricin toxin and ricin toxin-Ab complexes
    Authors: B Mooney, FJ Torres-Vel, J Doering, DJ Ehrbar, NJ Mantis
    J. Leukoc. Biol., 2019;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Binding Assay
  9. Functional genomic characterization of a synthetic anti-HER3 antibody reveals a role for ubiquitination by RNF41 in the anti-proliferative response
    Authors: JP Turowec, EWT Lau, X Wang, KR Brown, FA Fellouse, KK Jawanda, J Pan, J Moffat, S Sidhu
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  10. CD36 Differently Regulates Macrophage Responses to Smooth and Rough Lipopolysaccharide
    Authors: R Biedro?, A Peru?, S Józefowski
    PLoS ONE, 2016;11(4):e0153558.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  11. Oxidation by neutrophils-derived HOCl increases immunogenicity of proteins by converting them into ligands of several endocytic receptors involved in antigen uptake by dendritic cells and macrophages.
    Authors: Biedron R, Konopinski M, Marcinkiewicz J, Jozefowski S
    PLoS ONE, 2015;10(4):e0123293.
    Species: Mouse
    Sample Types: Protein
    Applications: ELISA Development (Detection)
  12. Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitro.
    Authors: Getmanova EV, Chen Y, Bloom L, Gokemeijer J, Shamah S, Warikoo V, Wang J, Ling V, Sun L
    Chem. Biol., 2006;13(5):549-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Extracellular Regulation of Bone Morphogenetic Protein Activity by the Microfibril Component Fibrillin-1.
    Authors: Wohl A, Troilo H, Collins R, Baldock C, Sengle G
    J Biol Chem, 0;291(24):12732-46.
    Species: Human
    Sample Types: Protein

FAQs

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Reviews for His Tag Horseradish Peroxidase-conjugated Antibody

Average Rating: 5 (Based on 4 Reviews)

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His Tag Horseradish Peroxidase-conjugated Antibody
By Anonymous on 11/26/2019
Application: WB Sample Tested: Cell Extracts Species: Bacterial

Western blot was made in 5% BSA, antibody worked beautifully at 1:1000 dilution.

Western Blot His Tag Horseradish Peroxidase-conjugated Antibody MAB050H
His Tag HRP-conjugated Antibody
By Anonymous on 07/13/2018
Application: TMB Detected Western Blot Sample Tested: 6xhis positive control,Protein Lysate Species: Bacterial

Excellent Antibody. Detects 6xhis positive control, almost instantaneously.

TMB Detected Western Blot His Tag HRP-conjugated Antibody MAB050H
His Tag HRP-conjugated Antibody
By Anonymous on 06/20/2018
Application: Simple Western Sample Tested: Purified protein Species: Human
Simple Western His Tag HRP-conjugated Antibody MAB050H
His Tag HRP-conjugated Antibody
By Anonymous on 05/17/2017
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Mouse
Western Blot His Tag HRP-conjugated Antibody MAB050H