hh His Tag PE-conjugated Antibody IC050P: R&D Systems

His Tag PE-conjugated Antibody

(2 citations)
(5 Reviews)
  
  • Specificity
    Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl-terminus.
  • Source
    Monoclonal Mouse IgG1 Clone # AD1.1.10
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    His-tagged peptide
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Protein by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with His-tagged protein were stained with Mouse Anti-His Tag PE‑conjugated Monoclonal Antibody (Catalog # IC050P, filled histogram) or isotype control antibody
(Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: His Tag
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.
  • Long Name:
    Histidine Tag
  • Alternate Names:
    6 His epitope tag; 6X His; 6X-His; H; HHHHHH epitope tag; HHHHHH tag; His Tag; HIS; polyHistidine; Poly-histidine
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. PTX3 binds MD-2 and promotes TRIF-dependent immune protection in aspergillosis.
    Authors: Bozza S, Campo S, Arseni B, Inforzato A, Ragnar L, Bottazzi B, Mantovani A, Moretti S, Oikonomous V, De Santis R, Carvalho A, Salvatori G, Romani L
    J Immunol, 2014;193(5):2340-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Evolutionarily conserved paired immunoglobulin-like receptor alpha (PILRalpha) domain mediates its interaction with diverse sialylated ligands.
    Authors: Sun Y, Senger K, Baginski TK, Mazloom A, Chinn Y, Pantua H, Hamidzadeh K, Ramani SR, Luis E, Tom I, Sebrell A, Quinones G, Ma Y, Mukhyala K, Sai T, Ding J, Haley B, Shadnia H, Kapadia SB, Gonzalez LC, Hass PE, Zarrin AA
    J. Biol. Chem., 2012;287(19):15837-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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Average Rating: 5 (Based on 5 reviews)

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Read what people are saying who have used His Tag PE-conjugated Antibody.

We have 5 reviews tested in 2 species: Human, Mouse.
We have 5 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry His Tag PE-conjugated Antibody IC050P
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Excellent
 Flow Mouse Anonymous 11/27/2017
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Image Details
Flow Cytometry His Tag PE-conjugated Antibody IC050P
Flow Cytometry: His Tag PE-conjugated Antibody [IC050P]

Summary

ApplicationFlow Cytometry
Sample TestedB16-F1 mouse melanoma cell line
SpeciesMouse
Flow Cytometry His Tag PE-conjugated Antibody IC050P
Enlarge
Excellent
 Flow Mouse Anonymous 11/18/2017
Show
Image Details
Flow Cytometry His Tag PE-conjugated Antibody IC050P
Flow Cytometry: His Tag PE-conjugated Antibody [IC050P]

Summary

ApplicationFlow Cytometry
Sample TestedRENCA
SpeciesMouse
Flow Cytometry His Tag PE-conjugated Antibody IC050P
Enlarge
Excellent
 Flow Human Anonymous 08/24/2017
Show
Image Details
Flow Cytometry His Tag PE-conjugated Antibody IC050P
Flow Cytometry: His Tag PE-conjugated Antibody [IC050P]

Summary

ApplicationFlow Cytometry
Sample TestedMEL-Juso human skin melanoma cell line
SpeciesHuman
Flow Cytometry His Tag PE-conjugated Antibody IC050P
Enlarge
Excellent
 Flow Mouse Anonymous 08/24/2017
Show
Image Details
Flow Cytometry His Tag PE-conjugated Antibody IC050P
Flow Cytometry: His Tag PE-conjugated Antibody [IC050P]

Summary

ApplicationFlow Cytometry
Sample TestedB16-F1 mouse melanoma cell line
SpeciesMouse
Flow Cytometry His Tag PE-conjugated Antibody ic050p
Enlarge
Excellent
 Flow Human Anonymous 03/28/2017
Show
Image Details
Flow Cytometry His Tag PE-conjugated Antibody ic050p
Flow Cytometry: His Tag PE-conjugated Antibody [ic050p]

Summary

ApplicationFlow Cytometry
Sample Tested293T human embryonic kidney cell line
SpeciesHuman

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