Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry, Bioassay, CAR-T (Flow Cytometry)

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # AD1.1.10
View all His Tag Primary Antibodies »

Product Specifications

Immunogen

His-tagged peptide

Specificity

Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl-terminus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for His Tag PE‑conjugated Antibody

Detection of His Tag antibody in HEK293 Human Cell Line Transfected with His-tagged Protein antibody by Flow Cytometry.

Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Protein by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with His-tagged protein were stained with Mouse Anti-His Tag PE-conjugated Monoclonal Antibody (Catalog # IC050P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Applications for His Tag PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with His-tagged protein fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Reviewed Applications

Read 12 reviews rated 5 using IC050P in the following applications:

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name

Histidine Tag

Alternate Names

polyHistidine

Additional His Tag Products

Product Documents for His Tag PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for His Tag PE‑conjugated Antibody

For research use only

Citations for His Tag PE‑conjugated Antibody

Customer Reviews for His Tag PE‑conjugated Antibody (12)

5 out of 5
12 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
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2 Stars
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1 Stars
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Customer Images

Showing  1 - 5 of 12 reviews Showing All
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  • His Tag PE-conjugated Antibody
    Name: Anonymous
    Application: Affinity Purification
    Sample Tested: MOUSE ANTIBODY DETECTION
    Species: Mouse
    Verified Customer | Posted 12/21/2018
  • His Tag PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: B16-F1 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 08/26/2018
  • His Tag PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: B16-F10 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 04/12/2018
  • His Tag PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: B16-F10 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 03/30/2018
  • His Tag PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: B16-F10 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 03/15/2018
  • His Tag PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: B16-F1 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 03/11/2018
  • His Tag PE-conjugated Antibody
    Name: Amit Kumar
    Application: Flow Cytometry
    Sample Tested: B16-F1 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 12/18/2017
  • His Tag PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: B16-F1 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 11/27/2017
    His Tag PE‑conjugated Antibody IC050P
  • His Tag PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: RENCA
    Species: Mouse
    Verified Customer | Posted 11/18/2017
    His Tag PE‑conjugated Antibody IC050P
  • His Tag PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: MEL-Juso human skin melanoma cell line
    Species: Human
    Verified Customer | Posted 08/24/2017
    His Tag PE‑conjugated Antibody IC050P
  • His Tag PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: B16-F1 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 08/24/2017
    His Tag PE‑conjugated Antibody IC050P
  • His Tag PE-conjugated Antibody
    Name: Md Munan Shaik
    Application: Flow Cytometry
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 03/28/2017
    His Tag PE‑conjugated Antibody IC050P

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Showing  1 - 5 of 12 reviews Showing All

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