His Tag PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC050P
Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Protein by Flow Cytometry.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (11)

His Tag PE-conjugated Antibody Summary

Specificity
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl-terminus.
Source
Monoclonal Mouse IgG1 Clone # AD1.1.10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
His-tagged peptide
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Protein by Flow Cytometry. View Larger

Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Protein by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with His-tagged protein were stained with Mouse Anti-His Tag PE‑conjugated Monoclonal Antibody (Catalog # IC050P, filled histogram) or isotype control antibody
(Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name
Histidine Tag
Alternate Names
6 His epitope tag; 6X His; 6X-His; H; HHHHHH epitope tag; HHHHHH tag; His Tag; HIS; polyHistidine; Poly-histidine

Product Datasheets

Citations for His Tag PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Blocking IL-2 Signal In Vivo with an IL-2 Antagonist Reduces Tumor Growth through the Control of Regulatory T Cells
    Authors: T Carmenate, Y Ortíz, M Enamorado, K García-Mar, J Avellanet, E Moreno, L Graça, K León
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  2. Pre-clinical validation of B cell maturation antigen (BCMA) as a target for T cell immunotherapy of multiple myeloma
    Authors: DX Bu, R Singh, EE Choi, M Ruella, S Nunez-Cruz, KG Mansfield, P Bennett, N Barton, Q Wu, J Zhang, Y Wang, L Wei, S Cogan, T Ezell, S Joshi, KJ Latimer, B Granda, WR Tschantz, RM Young, HA Huet, CJ Richardson, MC Milone
    Oncotarget, 2018;9(40):25764-25780.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  3. PTX3 binds MD-2 and promotes TRIF-dependent immune protection in aspergillosis.
    Authors: Bozza S, Campo S, Arseni B, Inforzato A, Ragnar L, Bottazzi B, Mantovani A, Moretti S, Oikonomous V, De Santis R, Carvalho A, Salvatori G, Romani L
    J Immunol, 2014;193(5):2340-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  4. Evolutionarily conserved paired immunoglobulin-like receptor alpha (PILRalpha) domain mediates its interaction with diverse sialylated ligands.
    Authors: Sun Y, Senger K, Baginski TK, Mazloom A, Chinn Y, Pantua H, Hamidzadeh K, Ramani SR, Luis E, Tom I, Sebrell A, Quinones G, Ma Y, Mukhyala K, Sai T, Ding J, Haley B, Shadnia H, Kapadia SB, Gonzalez LC, Hass PE, Zarrin AA
    J. Biol. Chem., 2012;287(19):15837-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

FAQs

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Reviews for His Tag PE-conjugated Antibody

Average Rating: 5 (Based on 11 Reviews)

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His Tag PE-conjugated Antibody
By Anonymous on 08/26/2018
Application: Flow Sample Tested: B16-F1 mouse melanoma cell line Species: Mouse

His Tag PE-conjugated Antibody
By Anonymous on 04/12/2018
Application: Flow Sample Tested: B16-F10 mouse melanoma cell line Species: Mouse

His Tag PE-conjugated Antibody
By Anonymous on 03/30/2018
Application: Flow Sample Tested: B16-F10 mouse melanoma cell line Species: Mouse

His Tag PE-conjugated Antibody
By Anonymous on 03/15/2018
Application: Flow Sample Tested: B16-F10 mouse melanoma cell line Species: Mouse

His Tag PE-conjugated Antibody
By Anonymous on 03/11/2018
Application: Flow Sample Tested: B16-F1 mouse melanoma cell line Species: Mouse

His Tag PE-conjugated Antibody
By Anonymous on 12/18/2017
Application: Flow Sample Tested: B16-F1 mouse melanoma cell line Species: Mouse

His Tag PE-conjugated Antibody
By Anonymous on 11/27/2017
Application: Flow Sample Tested: B16-F1 mouse melanoma cell line Species: Mouse

His Tag PE-conjugated Antibody
By Anonymous on 11/18/2017
Application: Flow Sample Tested: RENCA Species: Mouse

His Tag PE-conjugated Antibody
By Anonymous on 08/24/2017
Application: Flow Sample Tested: MEL-Juso human skin melanoma cell line Species: Human

His Tag PE-conjugated Antibody
By Anonymous on 08/24/2017
Application: Flow Sample Tested: B16-F1 mouse melanoma cell line Species: Mouse

His Tag PE-conjugated Antibody
By Anonymous on 03/28/2017
Application: Flow Sample Tested: 293T human embryonic kidney cell line Species: Human