His Tag PE-conjugated Antibody

  (3 citations)
(10 Reviews)
    
Datasheet / CoA / SDS
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
  • Specificity
    Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl-terminus.
  • Source
    Monoclonal Mouse IgG1 Clone # AD1.1.10
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    His-tagged peptide
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Intracellular Staining by Flow Cytometry
Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Protein by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with His-tagged protein were stained with Mouse Anti-His Tag PE‑conjugated Monoclonal Antibody (Catalog # IC050P, filled histogram) or isotype control antibody
(Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: His Tag
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.
  • Long Name:
    Histidine Tag
  • Alternate Names:
    6 His epitope tag; 6X His; 6X-His; H; HHHHHH epitope tag; HHHHHH tag; His Tag; HIS; polyHistidine; Poly-histidine
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Species
Applications
Sample Type
  1. Blocking IL-2 Signal In Vivo with an IL-2 Antagonist Reduces Tumor Growth through the Control of Regulatory T Cells
    Authors: T Carmenate, Y Ortíz, M Enamorado, K García-Mar, J Avellanet, E Moreno, L Graça, K León
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. PTX3 binds MD-2 and promotes TRIF-dependent immune protection in aspergillosis.
    Authors: Bozza S, Campo S, Arseni B, Inforzato A, Ragnar L, Bottazzi B, Mantovani A, Moretti S, Oikonomous V, De Santis R, Carvalho A, Salvatori G, Romani L
    J Immunol, 2014;193(5):2340-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Evolutionarily conserved paired immunoglobulin-like receptor alpha (PILRalpha) domain mediates its interaction with diverse sialylated ligands.
    Authors: Sun Y, Senger K, Baginski TK, Mazloom A, Chinn Y, Pantua H, Hamidzadeh K, Ramani SR, Luis E, Tom I, Sebrell A, Quinones G, Ma Y, Mukhyala K, Sai T, Ding J, Haley B, Shadnia H, Kapadia SB, Gonzalez LC, Hass PE, Zarrin AA
    J. Biol. Chem., 2012;287(19):15837-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 39  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 10 reviews)

Have you used His Tag PE-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used His Tag PE-conjugated Antibody.

We have 10 reviews tested in 2 species: Human, Mouse.
We have 10 review tested in 1 application: Flow Cytometry.

Filter by Ratings
Excellent
(10)
All Ratings
Filter by Applications
Flow
(10)
All Applications
Filter by Species
Human
(2)
Mouse
(8)
All Species

Images Ratings Applications Species Reviewed By Date Details
  Excellent
 Flow Mouse Anonymous 04/12/2018
Show

Summary

Application Flow Cytometry
Sample Tested B16-F10 mouse melanoma cell line
Species Mouse
  Excellent
 Flow Mouse Anonymous 03/30/2018
Show

Summary

Application Flow Cytometry
Sample Tested B16-F10 mouse melanoma cell line
Species Mouse
  Excellent
 Flow Mouse Anonymous 03/15/2018
Show

Summary

Application Flow Cytometry
Sample Tested B16-F10 mouse melanoma cell line
Species Mouse
  Excellent
 Flow Mouse Anonymous 03/11/2018
Show

Summary

Application Flow Cytometry
Sample Tested B16-F1 mouse melanoma cell line
Species Mouse
  Excellent
 Flow Mouse Anonymous 12/18/2017
Show

Summary

Application Flow Cytometry
Sample Tested B16-F1 mouse melanoma cell line
Species Mouse
Flow Cytometry His Tag PE-conjugated Antibody IC050P Array IC050P
Enlarge
Excellent
 Flow Mouse Anonymous 11/27/2017
Show
Image Details
Flow Cytometry His Tag PE-conjugated Antibody IC050P
Flow Cytometry: His Tag PE-conjugated Antibody [IC050P].

Summary

Application Flow Cytometry
Sample Tested B16-F1 mouse melanoma cell line
Species Mouse
Flow Cytometry His Tag PE-conjugated Antibody IC050P Array IC050P
Enlarge
Excellent
 Flow Mouse Anonymous 11/18/2017
Show
Image Details
Flow Cytometry His Tag PE-conjugated Antibody IC050P
Flow Cytometry: His Tag PE-conjugated Antibody [IC050P].

Summary

Application Flow Cytometry
Sample Tested RENCA
Species Mouse
Flow Cytometry His Tag PE-conjugated Antibody IC050P Array IC050P
Enlarge
Excellent
 Flow Human Anonymous 08/24/2017
Show
Image Details
Flow Cytometry His Tag PE-conjugated Antibody IC050P
Flow Cytometry: His Tag PE-conjugated Antibody [IC050P].

Summary

Application Flow Cytometry
Sample Tested MEL-Juso human skin melanoma cell line
Species Human
Flow Cytometry His Tag PE-conjugated Antibody IC050P Array IC050P
Enlarge
Excellent
 Flow Mouse Anonymous 08/24/2017
Show
Image Details
Flow Cytometry His Tag PE-conjugated Antibody IC050P
Flow Cytometry: His Tag PE-conjugated Antibody [IC050P].

Summary

Application Flow Cytometry
Sample Tested B16-F1 mouse melanoma cell line
Species Mouse
Flow Cytometry His Tag PE-conjugated Antibody ic050p Array IC050P
Enlarge
Excellent
 Flow Human Anonymous 03/28/2017
Show
Image Details
Flow Cytometry His Tag PE-conjugated Antibody ic050p
Flow Cytometry: His Tag PE-conjugated Antibody [ic050p].

Summary

Application Flow Cytometry
Sample Tested 293T human embryonic kidney cell line
Species Human

Please scroll the above reviews for more

Order Details

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies
R&D Systems Guarantee